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Protein Profiling Analyses in Arbuscular Mycorrhizal Symbiosis

  • Ghislaine Recorbet
  • Eliane Dumas-Gaudot*
Chapter

Abstract

Because proteins are well known as key effectors of plant responses to environmental cues including recognition, signaling, transport, and defense reactions, interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. The recent development of high throughput techniques has enabled large-scale analyses of symbiosis-related proteins. Different proteomic strategies have been established depending on the symbiotic stage targeted and on the abundance of mycorrhizal material. In mature mycorrhiza, sub-cellular proteomic approaches have been developed in the model legume Medicago truncatula to target symbiosis-related membrane proteins eligible for nutrient transport and signaling between symbionts upon arbuscule formation. Modifications in the M. truncatula root proteome during early stages of AM symbiosis have also been investigated by comparing protein patterns of non-inoculated roots and roots synchronized for appressorium formation. Concomitantly, proteomic approaches have been developed on in vitro-grown mycorrhiza to identify extraradical fungal proteins along with endomycorrhizins. The genome sequencing programs launched for M. truncatula and Glomus intraradices are likely to provide additional knowledge about AM symbiosis-related proteins.

Keywords

Arbuscular Mycorrhizal Mycorrhizal Root Appressorium Formation Actin Depolymerization Factor Root Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Ghislaine Recorbet
    • 1
  • Eliane Dumas-Gaudot*
    • 1
  1. 1.UMR 1088 INRA/CNRS 5184/UB Plante-Microbe-EnvironnementINRA/CMSEFrance

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