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Application of Yeast and Bacteria as Starter Cultures

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Biology of Microorganisms on Grapes, in Must and in Wine

The two main organisms involved in vinification Saccharomyces cerevisiae and Oenococcus oeni are somewhat unusual in selectively employing fermentative metabolism. They are also atypical in withstanding higher alcohol concentrations. Yeast are significant in winemaking because they carry out the alcoholic fermentation, causing the change from grape juice to wine by their metabolic activity as it was demonstrated by Louis Pasteur 1866. Besides having a major effect on the sugar content of juice or must yeast produces the most important wine volatiles, notably higher alcohols, fatty acids, and esters. Via its enzymatic properties it can also liberate grape derived bound aroma compounds. To a minor extend the statement above is also valuable for the wine lactic acid bacteria responsible for the malolactic fermentation. Malolactic fermentation is not only the simple decarboxylation of malic acid to lactic acid and CO2 resulting in a decrease of the total wine acidity. Utilizing wine as a substrate for growth, malolactic bacteria will remove some wine constituents and will produce others as a result of their metabolism. The metabolic activity of the MLB influences the aroma compounds of wine that are derived from fruit and the alcoholic fermentation, and it also confers biological stability upon the final product.

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References

  • Amerine MA, Berg HW, Kunkee RE, Ough CS, Singleton VL, Webb AD (1980) The Composition of Grapes and Wines. In: The Technology of Wine Making, 4th edn. Avi Publishing, Westport, CT

    Google Scholar 

  • Bartowski E, Henschke P (2004) The ‘buttery’ attribute of wine — diacetyl - desirability, spoilage and beyond. Int. J. Food Microbiol. 96:235–252

    Article  CAS  Google Scholar 

  • Beker MJ, Blumbergs JE, Ventina EJ, Rapoport AI (1984) Characteristics of cellular membranes at rehydration of dehydrated yeast Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 19:347–352

    Article  Google Scholar 

  • Bely M, Sablayrolles JM, Barre P (1991) Automatic detection of assimilable nitrogen deficiencies during alcoholic fermentation in enological conditions. J. Ferm. Bioeng. 70:246–252

    Article  Google Scholar 

  • Bisson LF, Butzke CE (2000) Diagnosis and rectification of stuck and sluggish fermentations. Am. J. Enol. Vitic. 51(2): 168–177

    CAS  Google Scholar 

  • Bou M, Powell C (2005) Chapter 6 — Strain Selection Techniques. In: Malolactic Fermentation in Wine — Understanding the Science and the Practice. Lallemand, Montréal, Canada

    Google Scholar 

  • Ciani M (1997) Role, Enological properties and potential use of non-Saccharomyces wine yeasts.Recent Res. Dev. Microbiol. 1:317–331

    Google Scholar 

  • Ciani M, Ferraro L (1996) Enhanced glycerol content in wines made with immobilized Candida stellata cells. Appl. Environ. Microbiol. 62:128–132

    PubMed  CAS  Google Scholar 

  • Clemente-Jimenez JM, Mingorance-Cazorla L, Martínez-Rodríguez S, Las Heras-Vázquez FJ, Rodríguez-Vico F (2005) Influence of sequential yeast mixtures on wine fermentation. Int. J. Food Microbiol. 98:301–308

    Article  PubMed  CAS  Google Scholar 

  • Davis CR, Wibowo D, Eschenbruch R, Lee TH, Fleet GH (1985) Practical implications of malolactic fermentation: A review. Am. J. Enol. Vitic. 36:290–301

    CAS  Google Scholar 

  • Degré R (1993) Selection and Commercial Cultivation of Wine Yeast and Bacteria. In: Fleet GH (ed) Wine Microbiology and Biotechnology. Harwood, Chur, Switzerland

    Google Scholar 

  • Dicks LMT, Dellaglio F, Collins MD (1995) Proposal to reclassify Leuconostoc oenosas Oenococcus oeni gen., comb. nov. Int. J. Syst. Bacteriol. 45:395–397

    Article  PubMed  CAS  Google Scholar 

  • Dittrich HH (1977) Mikrobiologie des Weines. Handbuch der Getränketechnologie. Ulmer Verlag Stuttgart

    Google Scholar 

  • Fischer U (2000) Gärunterbrechungen und Behebung von Gärstörungen. ATW-Bericht Nr. 97

    Google Scholar 

  • Fleet GH, Heard GM (1993) Yeasts — growth during fermentation. In: Fleet GH (ed.) Wine Microbiology and Biotechnology. Harwood Academic Publishers, Singapore, pp 27–54

    Google Scholar 

  • Fornairon-Bonnefond C, Demaretz V, Rosenfeld E, Salmon J-M (2002) Oxygen Addition and Sterol Synthesis in Saccharomyces cerevisiae During Enological Fermentation. J. Biosci.Bioeng. 93:176–182

    Article  PubMed  CAS  Google Scholar 

  • Graf M, Bannister M (1996) Restarting stuck fermentations. Vinquiry, 7795 Bell Road, Windsor, CA

    Google Scholar 

  • Grossmann M (2005) Spontangärung und Reinzuchthefegärung. In: Dittrich HH, Grossmann M (eds.) Mikrobiologie des Weines. Eugen Ulmer Verlag, Stuttgart, pp 102–118

    Google Scholar 

  • Henick-Kling T (1988) Yeast and Bacteria Control in Winemaking. In: Linskens HF, Jackson JF (eds.) Modern Methods of Plant Analysis. New Series, Volume 6 Wine Analysis. Springer, Berlin Heidelberg, pp 276–315

    Google Scholar 

  • Henschke PA (1997) Wine Yeasts. In: Zimmermann FK, Entian KD (eds.) Yeast Sugar Metabolism. Technomic, Lancaster, Pennsylvania, pp 527–560

    Google Scholar 

  • Henschke PA, Jiranek V (1993) Yeasts — Metabolism of Nitrogen Compounds. In: Fleet GH (ed.) Wine Microbiology and Biotechnology. Harwood, Singapore, pp 77–164

    Google Scholar 

  • Jiranek V, Langridge P, Henschke PA (1991) Yeast Nitrogen Demand: Selection Criterion for Wine Yeasts for Fermenting Low Nitrogen Musts. In: Rantz J (ed) Proceedings of the International Symposium on Nitrogen in Grapes and Wines. The American Society for Enology and Viticulture, Seattle, Washington, pp 266–269

    Google Scholar 

  • Julien A, Roustan JL, Dulau L, Sablayrolles JM (2000) Characterization of enological yeast strains:Evaluation of their nutrient requirements in nitrogen and oxygen. Am. J. Enol. Vitic. 51(3):302

    Google Scholar 

  • Julien A, Roustan JL, Dulau L, Sablayrolles JM (2001) Variabilité des besoins en oxygène et en azote assimilable suivant les souches de levures enologiques.. Revue Française d'enologie 189:20–22

    CAS  Google Scholar 

  • Kontkanen D, Inglis DL, Pickering GJ, Reynolds A (2004) Effect of Yeast Inoculation Rate, Acclimatization, and Nutrient Addition on Icewine Fermentation. Am. J. Enol. Vitic.55(4):363–370

    CAS  Google Scholar 

  • Krieger S (2005a) Proceedings 14th International Enology Symposium of International Association for Winery Technology and Management, 9th – 11th of May 2005, Thessaloniki (Greece)

    Google Scholar 

  • Krieger s (2005b) Chapter 12 — Determining When to Add Malolactic Bacteria. In: Malolactic Fermentation in Wine — Understanding the Science and the Practice. Lallemand, Montréal, Canada

    Google Scholar 

  • Krieger S (2006) Timing of malolactic fermentation in white wine — a tool to control fruitiness.Proceeding of the Sixth International Cool Climate Symposium for Viticulture and Oenology, 2006 New Zealand

    Google Scholar 

  • Lafon-Lafourcade S, Geneix C, Ribéreau-Gayon P. (1984) Inhibition of alcoholic fermentation of grape must by fatty acids produced by yeasts and their elimination by yeast ghosts. Appl.Environ. Microbiol. 47:1246–1249

    PubMed  CAS  Google Scholar 

  • Lallemand Winemaking Up-date (2007) The contribution of malolactic starter cultures to the sensory quality of wine. http://www.lallemandwine.com Number 1-2007

  • Languet P, Ortiz-Julien A, Salmon JM (2006) Sequential Use of Non-Saccharomyces and Saccharomyces Yeast to Enhance Organoleptic Properties of Wine. In: 3rd International Viticulure and Enology Conference, Cape Town, South Africa

    Google Scholar 

  • Leske P, Henschke P (1996) Procedure for restarting stuck fermentations. AWRI Tech. Rev.104:13–16

    Google Scholar 

  • Luparia V, Soubeyrand V, Berges T, Julien A, Salmon J-M (2004) Assimilation of Grape Phytosterols by Saccharomyces cerevisiae and Their Impact on Enological Fermentations.Appl. Microbiol. Biotechnol. 65:25–32

    Article  PubMed  CAS  Google Scholar 

  • Martineau B, Henick-Kling T (1995) Performance and diacetyl production of commercial strains of malolactic bacteria in wine. J. Appl. Bacteriol. 78:526–536

    CAS  Google Scholar 

  • Monk PR (1986) Rehydration and propagation of active dry wine yeast. Aust. Wine Ind. J. 1:3–5

    Google Scholar 

  • Moreno JJ, Millán C, Ortega JM, Medina M (1991) Analytical differentiation of wine fermentations using pure and mixed yeast cultures. J. Ind. Microbiol. Biotechnol. 7:181–189

    CAS  Google Scholar 

  • Morenzoni R (2005) Chapter 2 — Introduction. In: Malolactic Fermentation in Wine — Understanding the Science and the Practice. Lallemand, Montréal, Canada

    Google Scholar 

  • Müller-Thurgau H (1891) Ergebnisse neuer Untersuchungen auf dem Gebiete der Weinbereitung.Weinbau und Weinhandel 9:421–428

    Google Scholar 

  • Müller-Thurgau H, Osterwalder A (1913) Die Bakterien im Wein und Obstwein und die dadurch verursachten Veränderungen. Zbl. Bakteriologie II 36:129–338

    Google Scholar 

  • Nielsen JC, Richelieu M (1999). Control of flavor development in wine during and after malolactic fermentation by Oenococcus oeni. Appl. Environ. Microbiol. 65:740– 745

    PubMed  CAS  Google Scholar 

  • Pasteur L (1866) Etudes sur le vin, ses maladies, causes qui les provoquent. Imprimerie Impériale, Paris

    Google Scholar 

  • Prahl C (1989) La décarboxylation de l'acide L-malique dans le moût par l'ensemencement de lactobacilles homofermentaires. Revue des Enologues et des Techn. Vitivin. Enolog.54:13–17

    Google Scholar 

  • Radler F (1963) Über die Milchsäurebakterien des Weines und den biologischen Säureabbau.Übersicht. II. Physiologie und Ökologie der Bakterien. Vitis 3:207–236

    CAS  Google Scholar 

  • Radler F, Dietrich K, Schönig I (1985) Mikrobiologische Prüfung von Trockenhefe-präparaten für die Weinbereitung. Deutsche Lebensmittelrundschau 3:73–77

    Google Scholar 

  • Sablayrolles JM, Dubois C, Manginot C, Roustan JL, Barre P (1996) Effectiveness of combined ammoniacal nitrogen and oxygen additions for completion of sluggish and stuck wine fermentations.. J. Ferm. Bioeng. 82:377–381

    Article  CAS  Google Scholar 

  • Schütz M, Gafner J (1993) Analyses of yeast diversity during spontaneous and induced alcoholic fermentations. J. Appl. Bacteriol. 75:551–558

    Google Scholar 

  • Soubeyrand V (2005) Formation of Micella Containing Solubilized Sterols During Rehydration of Active Dry Yeasts Improves Their Fermenting Capacity. J. Agric. Food Chem.53:8025–8032

    Article  PubMed  CAS  Google Scholar 

  • Specht G (2005) Chapter 11 — Guidelines for using commercial strains. In: Malolactic Fermentation in Wine — Understanding the Science and the Practice. Lallemand, Montréal, Canada

    Google Scholar 

  • Sütterlin KA, Hoffmann-Boller P, Gafner J (2004) Kurieren von Gärstockungen mit der fructophilen Weinhefe Zygosaccharomyces bailii. In Proceedings of the 7th International Symposium on Innovations in Enology, Intervitis Interfructa 2004 10. + 11.05.2004

    Google Scholar 

  • Ugliano M, Moio L (2006) The influence of malolactic fermentation and Oenococcus oenistrain on glycosidic aroma precursors and related volatile compounds of red wine. J. Sci. Food Agric.86:2468–2476

    Article  CAS  Google Scholar 

  • Ugliano M, Geneovese A, Moio L (2003) Hydrolysis of wine aroma precursors during malolactic fermentation with four commercial starter cultures of Oenococcus oeni. J. Agric. Food Chem.51:5073–5078

    Article  PubMed  CAS  Google Scholar 

  • Wibowo D, Eschenbruch R, Davis DR, Fleet GH, Lee TH (1985) Occurrence and growth of lactic caid bacteria in wine: A review. Am. J. Enol. Vitic. 36:302–313

    CAS  Google Scholar 

  • Wilkinson J (1986) Secondary Fermentation and Maturation. In: Lee TH, Lester DC (eds.) Production of wine by the methode champenoise. Proc. Seminar Aust. Soc. Vitic. Oenol. 15 Nov 1985. The Australian Wine Research Institute, Glen Osmond, South Australia, pp 125–131

    Google Scholar 

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  1. Lallemand Inc., In den Seiten 53, Korntal-Münchingen, 70825, Germany

    Sibylle Krieger-Weber

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  1. Sibylle Krieger-Weber
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  1. Institute of Microbiology and Wine Research, Johannes Gutenberg-University, Becherweg 15, Mainz, 55099, Germany

    Helmut König , Gottfried Unden  & Jürgen Fröhlich ,  & 

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Krieger-Weber, S. (2009). Application of Yeast and Bacteria as Starter Cultures. In: König, H., Unden, G., Fröhlich, J. (eds) Biology of Microorganisms on Grapes, in Must and in Wine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85463-0_27

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