Skip to main content

Wine-associated microorganisms like yeasts and lactic acid bacteria produce a wide spectrum of exoenzymes (e.g., proteases, glucanases, glucosidases, esterases, lipases, tannases, phenoloxidases). The individual members of the wine microflora display their specific enzyme spectrum at different stages of vinification. Glucanases and phenoloxidases produced by Botrytis cinerea as part of the grape infection process may enter the must and alter its composition. Polysaccharide-degrading enzymes mainly produced by non-Saccharomyces cerevesiae yeasts and members of the genera Lactobacillus and Pediococcus dominate in the early stage of fermentation. Strains of Oenoccoccus oeni produce esterases and tannases, which may affect wine taste after completion of the alcoholic fermentation. Our present knowledge about the ecological relevance of the microbial exoenzymes, their effects on the final product, and their biotechnological potential for wine-making is far from complete and efforts further studies.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 279.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Adams DO (2006) Phenolics and ripening in grape berries. Am J Enol Viticult 57: 249–256

    CAS  Google Scholar 

  • Barbagallo RN, Spagna G, Palmieri R, Torriani S (2004) Assessment of β-glucosidase activity in selected wild strains of Oenococcus oeni for malolactic fermentation. Enzyme Microb Technol 34: 292–296

    Article  CAS  Google Scholar 

  • Boido E, Lloret A, Medina K, Carreau F, Dellacassa E (2002) Effect of beta-glycosidase activity of Oenococcus oeni on the glycosylated flavor precursors of Tannat wine during malolactic fermentation. J Agric Food Chem 50: 2344–2349

    Article  PubMed  CAS  Google Scholar 

  • Brenna O, Bianchi E (1994) Immobilized laccase for phenolic removal in must and wine. Biotechnol Lett 16: 35–40

    Article  CAS  Google Scholar 

  • Charoenchai C, Fleet GH, Heschke PA, Todd BEN (1997) Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust J Grape Wine Res 3: 2–8

    Article  CAS  Google Scholar 

  • Cherry JM, Ball C, Weng S, Juvik G, Schmidt R, Adler C, Dunn B, Dwight S, Riles L, Mortimer RH, Botstein D (1997) Genetic and physical maps of Saccharomyces cerevisiae. Nature 387: 67–73

    Article  PubMed  CAS  Google Scholar 

  • Cid VJ, Duran A, Delrey F, Snyder MP, Nombela C, Sanchez M (1995) Molecular-basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59: 345–386

    PubMed  CAS  Google Scholar 

  • Claus H (2004) Laccases: structure, reactions, distribution. Micron 35: 93–96

    Article  PubMed  CAS  Google Scholar 

  • Claus H (2007) Extracellular enzymes and peptides of lactic acid bacteria: significance for vinifi-cation. Deut Lebensm-Rundsch 11: 505–511

    Google Scholar 

  • Claus H, Decker H (2006) Bacterial tyrosinases. Syst Appl Microbiol 29: 3–14

    Article  PubMed  CAS  Google Scholar 

  • Claus H, Filip Z (1988) Behaviour of phenoloxidases in the presence of clays and other soil-related adsorbents. Appl Microbiol Biotechnol 28: 506–511

    Article  CAS  Google Scholar 

  • Daenen L, Saison D, Sterckx F, Delvaux FR, Verachtert H, Derdelelinckx G (2008) Screening and evaluation of the glucoside hydrolase activity in Saccharomyces and Brettanomyces brewing yeasts. Appl Microbiol 104: 478–488

    CAS  Google Scholar 

  • Davis CR, Wibowo D, Fleet GH, Lee TH (1988) Properties of wine lactic acid bacteria: their potential enological significance. Am J Enol Viticult 39: 137–142

    CAS  Google Scholar 

  • Delaquis P, Cliff M, King B, Girard B, Hall J, Reynolds A (2000) Effect of two commercial malolactic cultures on the chemical and sensory properties of Chancellor wines with different yeasts and fermentation temperatures. Am J Enol Viticult 51: 42–48

    CAS  Google Scholar 

  • Delcroix A, Gunata Z, Sapis LC, Salmon JM, Baynone C (1994) Glycosidase activities of three enological yeast strains during winemaking: effect on the terpenol content of Muscat wine. Am J Enol Viticult 45: 291–296

    CAS  Google Scholar 

  • Dewey FM, Hill M, DeScenzo R (2008) Quantification of Botrytis and laccase in winegrapes. Am J Enol Viticult 59: 47–54

    CAS  Google Scholar 

  • D'Incecco N, Bartowsky EJ, Kassara S, Lante A, Spettoli P, Henschke PA (2004) Release of gly-cosidically bound flavour compounds from Chardonnay by Oenococcus oeni during malolactic fermentation. Food Microbiol 21: 257–265

    Article  CAS  Google Scholar 

  • Dubourdieu D, Ribéreau-Gayon P, Fournet B (1981) Structure of the extracellular β-d-glucan from Botrytis cinerea. Carbohydr Res 93: 294–299

    Article  CAS  Google Scholar 

  • Duerksen JD, Halvorson H (1958) Purification and properties of an inducible beta-glucosidase of yeast. J Biol Chem 233: 1113–1120

    PubMed  CAS  Google Scholar 

  • Eder R, Wendelin S (2002) Phenolzusammensetzung und antioxidative Kapazität von Trauben und Weinen. ALVA Jahrestagung 2002, Klosterneuburg, pp 293–296

    Google Scholar 

  • Espín JC, Wichers HJ (2000) Study of the oxidation of resveratrol catalyzed by polyphenol oxidase. Effect of polyphenol oxidase, laccase and peroxidase on the antiradical capacity of resveratrol. J Food Biochem 24: 225–250

    Article  Google Scholar 

  • Farías ME, Manca de Nadra MC (2000) Purification and partial characterization of Oenococcus oeni exoprotease. FEMS Microbiol Lett 185: 263–266

    PubMed  Google Scholar 

  • Fernández M, Úbeda JF, Briones AI (2000) Typing of non-Saccharomyces yeasts with enzymatic activities of interest in wine-making. Int J Food Microbiol 59: 29–36

    Article  PubMed  Google Scholar 

  • Ferreira AM, Climaco MC, Faia AM (2001) The role of non-Saccharomyces species in releasing glycosidic bound fraction of grape aroma components – a preliminary study. J Appl Microbiol 91: 67–71

    Article  CAS  Google Scholar 

  • Fia G, Giovani G, Rosi I (2005) Study of β-glucosidase production by wine-related yeasts during alcoholic fermentation. A new rapid fluorimetric method to determine enzymatic activity. J Appl Microbiol 99: 509–517

    Article  PubMed  CAS  Google Scholar 

  • Folio P, Ritt JF, Alexandre H, Remize F (2008) Characterization of EprA, a major extracellular protein of Oenococcus oeni with protease activity. Int J Food Microbiol 127: 26–31

    Article  PubMed  CAS  Google Scholar 

  • Fragopoulou E, Antonopoulou S, Demopoulos CA (2002) Biologically active lipids with antia-therogenic properties from white wine and must. J Agric Food Chem 50: 2684–2694

    Article  PubMed  CAS  Google Scholar 

  • Fugelsang KC, Edwards CG (2007) Wine microbiology: practical applications and procedures, 2nd edn. Springer, Berlin

    Google Scholar 

  • Gallander JF, Peng AC (1980) Lipid and fatty acid composition of different wine grapes. Am J Enol Viticult 31: 24–27

    CAS  Google Scholar 

  • Gil JV, Manzanares P, Genoves S, Valles S, Gonzalez-Candelas L (2005) Over-production of the major exoglucanase of Saccharomyces cerevisiae leads to an increase in the aroma of wine. Int J Food Microbiol 103: 57–68

    Article  PubMed  CAS  Google Scholar 

  • Grassin C, Dubourdieu D (1989) Quantitative determination of Botrytis laccase in musts and wines by the syringaldazine test. J Sci Food Agric 48: 369–376

    Article  CAS  Google Scholar 

  • Grimaldi A, McLean H, Jiranik V (2000) Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium Oenococcus oeni. Am J Enol Viticult 51: 362–369

    CAS  Google Scholar 

  • Grimaldi A, Bartowsky R, Jiranik V (2005a) A survey of glycosidase activities of commercial wine strains of Oenococcus oeni. Int J Food Microbiol 105: 233–244

    Article  CAS  Google Scholar 

  • Grimaldi A, Bartowsky E, Jiranik V (2005b) Screening of Lactobacillus spp. and Pediococcus spp. for glycosidase activities that are important in oenology. J Appl Microbiol 99: 1061–1069

    Article  CAS  Google Scholar 

  • Guilloux-Benatier M, Pageault O, Feuillat M (2000) Lysis of yeast cells by Oenococcus enzymes. J Ind Microbiol Biotechnol 25: 193–197

    Article  CAS  Google Scholar 

  • Izzo R, Muratore G (1993) Seed lipids from some varieties of grapes grown in Sicily: note 1. Fatty acid composition. Riv Ital Sostanze Grasse 70: 601–604

    CAS  Google Scholar 

  • Jacobsen JL (2006) Introduction to wine laboratory practices and procedures. Springer, Berlin

    Google Scholar 

  • Kennedy JA, Saucier C, Glories Y (2006) Grape and wine phenolics: history and perspective. Am J Enol Viticult 57: 239–248

    CAS  Google Scholar 

  • Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma: a review. S Afr J Enol Viticult 21: 97–129

    CAS  Google Scholar 

  • Llaubéres RM, Richard B, Lonvaud A, Dubourdieau D, Fournet B (1990) Structure of extracellular β-d-glucan from Pediococcus sp., a wine lactic bacterium. Carbohydr Res 203: 103–107

    Article  PubMed  Google Scholar 

  • Macheix JJ, Sapis JC, Fleuriet A (1991) Phenolic compounds and polyphenoloxidase in relation to browning in grapes and wines. Crit Rev Food Sci Nutr 30: 441–486

    Article  PubMed  CAS  Google Scholar 

  • Maicas S, Gil J V, Pardo I, Ferrer S (1999) Improvement of volatile composition of wines by controlled addition of malolactic bacteria. Food Res Int 32: 491–496

    Article  CAS  Google Scholar 

  • Maier G, Dietrich H, Wucherpfennig K (1990) Winemaking without SO2 – with the aid of enzymes? Weinwirtschaft–Technik 126: 18–22

    Google Scholar 

  • Manca de Nadra MC, Strasser de Saad AM (1995) Polysaccharide production by Pediococcus pentosaceus from wine. Int J Food Microbiol 27: 101–106

    Article  PubMed  CAS  Google Scholar 

  • Manca de Nadra MC, Farías NE, Moreno-Arribas V, Pueyo E, Polo MC (1997) Proteolytic activity of Leuconostoc oenos: effect on proteins and polypeptides from white wine. FEMS Microbiol Lett 150: 135–139

    Article  CAS  Google Scholar 

  • Manca de Nadra MC, Farías NE, Moreno-Arribas V, Pueyo E, Polo MC (1999) A proteolytic effect of Oenococcus oeni on the nitrogenous macromolecular fraction of red wine. FEMS Microbiol Lett 174: 491–496

    Article  Google Scholar 

  • Mansfield AK, Zoecklein BW, Whitton RS (2002) Quantification of glycosidase activity in selected strains of Brettanomyces bruxellensis and Oenococcus oeni. Am J Enol Viticult 53: 303–307

    CAS  Google Scholar 

  • Mateo JJ, DiStefano R (1997) Description of the beta-glucosidase activity of wine yeasts. Food Microbiol 14: 583–591

    Article  CAS  Google Scholar 

  • Matthews A, Grimaldi A, Walker M, Bartowsky E, Grbin P, Jiranek V (2004) Lactic acid bacteria as a potential source of enzymes for use in vinification. Appl Environ Microbiol 70: 5715–5731

    Article  PubMed  CAS  Google Scholar 

  • Matthews A, Grbin PR, Jiranek V (2006) A survey of lactic acid bacteria for enzymes of interest to oenology. Aust J Grape Wine Res 12: 235–244

    Article  CAS  Google Scholar 

  • Matthews A, Grbin PR, Jiranek V (2007) Biochemical characterisation of the esterase activities of wine lactic bacteria. Appl Microbiol Biotechnol 77: 329–337

    Article  PubMed  CAS  Google Scholar 

  • Mayer AM, Staples RC (2002) Laccase: new functions for an old enzyme. Phytochemistry 60: 551–565

    Article  PubMed  CAS  Google Scholar 

  • Minussi RC, Pastore GM, Durán N (2002) Potential applications of laccase in the food industry. Trends Food Sci Technol 13: 205–216

    Article  CAS  Google Scholar 

  • Minussi RC, Rossi M, Bolgna L, Rotilio D, Pastore GM, Durán N (2007) Phenols removal in musts: strategy for wine stabilization by laccase. J Mol Catal B: Enzymatic 45: 102–107

    Article  CAS  Google Scholar 

  • Nebreda AR, Villa TG, Villanueva JR, Delrey F (1986) Cloning of genes related to exo-beta-glu-canase production in Saccharomyces cerevisiae – characterization of an exo-beta-glucanase structural gene. Gene 47: 245–259

    Article  PubMed  CAS  Google Scholar 

  • Oak MH, Bedoi JE, Schini-Kerth V (2005) Antiangiogenic properties of natural polyphenols from red wine and green tea. J Nutr Biochem 16: 1–8

    Article  PubMed  CAS  Google Scholar 

  • Olivero I, Hernandez LM, Larriba G (1985) Regulation of beta-exoglucanase activity production by Saccharomyces cerevisiae in batch and continuous culture. Arch Microbiol 143: 143–146

    Article  PubMed  CAS  Google Scholar 

  • Pfeiffer P, Schlander M, König H (2007) Detection and production of pyroglutamic acid ethyl ester in wine. Deut Lebensm-Rundsch 103: 8–10

    CAS  Google Scholar 

  • Pueyo E, Martinez-Rodriguez A, Polo MC, Santa-Maria G, Bartomé B (2000) Release of lipids during yeast autolysis in a model wine. J Agric Food Chem 48: 116–122

    Article  PubMed  CAS  Google Scholar 

  • Quatrini P, Marineo S, Puglia AM (2006) The beta-glucosidase encoding gene from yeast strains Isolated from Sicilian musts and wines (DQ010949). Center for Biotechnology Information (NCBI), Bethesda, MD. Available at http://www.ncbi.nlm.nih.gov/, accessed October 15, 2006

    Google Scholar 

  • Rapp A, Mandery H (1986) Wine aroma. Experientia 42: 873–884

    Article  CAS  Google Scholar 

  • Ridruejo JC, Munoz MD, Andaluz E, Larriba G (1989) Inhibition of yeast exoglucanases by glu-cosidase inhibitors. Biochim Biophys Acta 993: 179–185

    PubMed  CAS  Google Scholar 

  • Rodriguez ME, Lopes CA, Broock M, Valles S, Ramon D, Caballero AC (2004) Screening and typing of Patagonian wine yeasts for glycosidase activities. J Appl Microbiol 96: 84–95

    Article  PubMed  CAS  Google Scholar 

  • Rojas A, Romeu A (1996) A sequence analysis of the beta-glucosidase sub-family B. FEBS Lett 378: 93–97

    Article  PubMed  CAS  Google Scholar 

  • Rollán GC, Farías ME, Manca de Nadra MC (1993) Protease production by Leuconostoc oenos strains isolated from wine. World J Microbiol Biotechnol 9: 587–589

    Article  Google Scholar 

  • Rollán GC, Farías ME, Manca de Nadra MC (1995) Characterization of two extracellular proteases from Leuconostoc oenos. World J Microbiol Biotechnol 11: 153–155

    Article  Google Scholar 

  • Rollán GC, Farías ME, Strasser de Sand AM, Manca de Nadra MC (1998) Exoprotease activity of Leuconostoc oenos in stress condition. J Appl Microbiol 85: 219–233

    Article  Google Scholar 

  • Rosi I, Vinella M, Domizio P (1994) Characterization of beta-glucosidase activity in yeasts of oenological origin. J Appl Bacteriol 77: 519–527

    PubMed  CAS  Google Scholar 

  • Saerens SMG, Delvaux F, Verstrepen KJ, Van Dijck P, Thevelein JM, Delvaux FR (2008) Parameters affecting ethyl ester production by Saccharomyces cerevisiae during fermentation. Appl Environ Microbiol 74: 454–461

    Article  PubMed  CAS  Google Scholar 

  • Somers TC (1984) Botrytis cinerea – consequences for red wines. Aust Grape Wine 244: 80–85

    Google Scholar 

  • Spagna G, Barbagallo RN, Palmeri R, Restuccia C, Giudici P (2002) Properties of endogenous beta-glucosidase of a Saccharomyces cerevisiae strain isolated from Sicilian musts and wines. Enzyme Microb Technol 31: 1030–1035

    Article  CAS  Google Scholar 

  • Strauss MLA, Jolly NP, Lambrechts MG, van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol 91: 182–190

    Article  PubMed  CAS  Google Scholar 

  • Strong PJ, Burgess JE (2007) Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase. Water Sci Technol 56: 179–186

    PubMed  CAS  Google Scholar 

  • Suzuki K, Yabe T, Maruyama Y, Abe K, Nakajima T (2001) Characterization of recombinant yeast exo-beta-1,3-glucanase (Exg 1p) expressed in Escherichia coli cells. Biosci Biotechnol Biochem 65: 1310–1314

    Article  PubMed  CAS  Google Scholar 

  • Ugliano M, Bartowsky EJ, McCarthy J, Moio L, Henschke PA (2006) Hydrolysis and transformation of grape glycosidically bound volatile compounds during fermentation with three Saccharomyces yeast strains. J Agric Food Chem 54: 6322–6331

    Article  PubMed  CAS  Google Scholar 

  • van Rensburg P, Pretorius IS (2000) Enzymes in winemaking: harnessing natural catalysts for efficient biotransformation – a review. S Afr J Enol Viticult 21: 52–73

    Google Scholar 

  • Vaquero I, Macrobal Á, Muñoz R (2004) Tannase activity by lactic bacteria isolated from grape must and wine. Int J Food Microbiol 96: 199–204

    Article  PubMed  CAS  Google Scholar 

  • Velasco SE, Yebra MJ, Monedero V, Ibarburu I, Dueñas MT, Irastorza A (2007) Influence of the carbohydrate source on β-glucan production and enzyme activities involved in sugar metabolism in Pediococcus parvulus 2.6. Int J Food Microbiol 115: 325–334

    Article  PubMed  CAS  Google Scholar 

  • Villena MA, Iranzo JFU, Otero RRC, Perez AIB (2005) Optimization of a rapid method for studying the cellular location of beta-glucosidase activity in wine yeasts. J Appl Microbiol 99: 558–564

    Article  CAS  Google Scholar 

  • Villetaz JC, Steiner D, Trogus H (1984) The use of β-glucanase as an enzyme in wine clarification and filtration. Am J Enol Viticult 35: 253–256

    Google Scholar 

  • Vivas NA, Lonvaud-Funel A, Glories Y (1997) Effect of phenolic acids and anthocyanins on growth, viability and malolactic activity of a lactic bacterium. Food Microbiol 14: 291–300

    Article  CAS  Google Scholar 

  • Wibowo D, Eschenbruch R, Davis CR, Fleet GH, Lee TH (1985) Occurrence and growth of lactic bacteria in wine. Am J Enol Viticult 36: 302–313

    CAS  Google Scholar 

  • Winterhalter P, Skouroumounis GK (1997) Glycoconjugated aroma compounds: occurrence, role and biotechnological transformation. Adv Biochem Eng Biotechnol 55: 74–99

    Google Scholar 

  • Yanai T, Sato N (1999) Isolation and properties of β-glucosidase produced by Debaryomyces hansenii and its application in winemaking. Am J Enol Viticult 50: 231–235

    CAS  Google Scholar 

  • Younis OS, Stewart GG (1998) Sugar uptake and subsequent ester and higher alcohol production by Saccharomyces cerevisiae. J Inst Brew 104: 255–264

    CAS  Google Scholar 

  • Zeemann WJ, Snyman P, van Wyk SJ (1982) The influence of yeast strain and malolactic fermentation on some volatile bouquet substances and on quality of table wines. In: Proceedings of the Davis, Grape and Wine Centennial Symposium 1980, University of California, pp 242–271

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Claus, H. (2009). Exoenzymes of Wine Microorganisms. In: König, H., Unden, G., Fröhlich, J. (eds) Biology of Microorganisms on Grapes, in Must and in Wine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-85463-0_14

Download citation

Publish with us

Policies and ethics