Abstract
Aim: The two main obstacles of successfully grafting tissue engineered skin are the shortage of the resource of the seed cell and the long process of vascularization of grafted skin. This article investigates the feasibility and condition of differentiation of the human marrow mesenchymal stem cells (MSCs) into the vascular endothelial-like cells in vitro. Methods: The MSCs were harvested, isolated and purified by combining density gradient centrifugation with adhering method. The cultured cells in induce group were treated with 10µg/L vascular endothelial growth factor(VEGF) and 5µg/L basic fibroblast growth factor(bFGF). No VEGF and bFGF were added in control group. The morphological changes of the cells were observed with inverted phase contrast microscope. The expression of CD34, VIII factor were determined by the immunocytochemistry methods. The transmission electron microscope was used to further explore the ultrastructure characteristics of the cultered cells. Results: The cells induced by VEGF and bFGF showed the morphological changes from long fusiform-shaped to short fusiform or flat shape, which was consistent with the significant characteristic of endothelium cells. The expression of CD34, VIII factor were positive in the induced cells, which were negative in the control group. The Weibel-Palade body could be found in the cytoplasm of some induced cells. Conclusion: Combined VEGF with bFGF can induce MSCs differentiate into vascular endothelial- like cells in vitro, which may be unfold the potential applied prospects for vascularization and application of tissue engineered skin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Tremain N, Korkko J, Ibberson D, et al. MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells revealsm RNAs of multiple cell lineages. Stem Cells, 2001,19(5): 408–418
Bin F, Yinglong L, Nin X,et al. Construction of tissue-engineered homograft bioprosthetic heart valves in vitro. ASAIOJ,2006,52(3):303–309
Oswald J, Boxberger S, Jorgensen B. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells, 2004, 22:377–384
Roberts DM, Kearney JB, Johnson JH,et al. The vascular endothelial growth factor (VEGF) receptor Flt-1 (VEGFR-1)modulates Flk-1 (VEGFR-2) signaling during blood vessel formation. Am J Pathol, 2004,164(5):1531
Kearney JB, Kappas NC, Ellerstrom C,et al. The VEGF receptor flt-1 (VEGFR-1) is a positive modulator of vascular sprout formation and branching morphogenesis. Blood,2004,103(12):4527–4535
Wang M, Crisostomo PR, Herring C, et al.Human progenitor cells from bone marrow or adipose tissue produce VEGF, HGF, and IGF-I in response to TNF by a p38 MAPK-dependent mechanism. Am J Physiol Regul Integr Comp Physiol, 2006,291(4):R880–884
Zeng Q, Li X, Beck G, et al. Growth and differentiation factor-5 (GDF-5) stimulates osteogenic differentiation and increases vascular endothelial growth factor (VEGF) levels in fat-derived stromal cells in vitro. Bone. 2007,40(2):374–381
Crisostomo PR, Wang M, Herring CM, et al. Gender differences in injury induced mesenchymal stem cell apoptosis and VEGF, TNF, IL-6 expression: Role of the 55 kDa TNF receptor (TNFR1). J Mol Cell Cardiol. 2007,42(1):142–149
Kim S, Honmou O, Kato K, et al.Neural differentiation potential of peripheral blood-and bone-marrow-derived precursor cells.Brain Res,2006, 1123(1):27–33
Schmidt A, Ladage D, Schinkothe T,et al. Basic fibroblast growth factor controls migration in human mesenchymal stem cells.Stem Cells,2006,24(7):1750–1758
Ong SY, Dai H, Leong KW. Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture. Biomaterials, 2006,27(22):4087–4097
Mayer H, Bertram H, Lindenmaier W,et,al.Vascular endothelial growth factor (VEGF-A) expression in human mesenchymal stem cells: autocrine and paracrine role on osteoblastic and endothelial differentiation. J Cell Biochem,2005, 95(4):827–839
Okuyama H, Krishnamachary B, Zhou YF,et,al.Expression of vascular endothelial growth factor receptor 1 in bone marrow-derived mesenchymal cells is dependent on hypoxia-inducible factor. J Biol Chem,2006,281(22):15554–15563
Hideshima T, Podar K, Chauhan D, et al. Cytokines and signal transduction. Best Pract Res Clin Haematol, 2005,18(4):509–524
Jiang W, Ma A, Wang T, et al.Intravenous transplantation of mesenchymal stem cells improves cardiac performance after acute myocardial ischemia in female rats. Transpl Int,2006,19(7):570–580
Miyahara Y, Nagaya N, Kataoka M, et al.Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction.Nat Med,2006,12(4):459–465
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Liu, D., Zhang, X., Li, X., Zhang, Z., Guo, G. (2008). Differentiation of the Human Marrow Mesenchymal Stem Cells into Vascular Endothelium-like Cells in vitro. In: Peng, Y., Weng, X. (eds) 7th Asian-Pacific Conference on Medical and Biological Engineering. IFMBE Proceedings, vol 19. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-79039-6_22
Download citation
DOI: https://doi.org/10.1007/978-3-540-79039-6_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-79038-9
Online ISBN: 978-3-540-79039-6
eBook Packages: EngineeringEngineering (R0)