Andrology pp 61-85 | Cite as

Physiology of Sperm Maturation and Fertilization

  • Trevor G CooperEmail author
  • Ching-Hei Yeung

Fertilization is the event that brings together the cellular content of both haploid gametes in the formation of a new individual.


Zona Pellucida Acrosome Reaction Human Epididymal Protein Testicular Spermatozoon Sperm Maturation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Ariel M, Cedar H, McCarry J (1994) Developmental changes in methylation of spermatogenesis-specific genes include reprogramming in the epididymis. Nature 7:59–63Google Scholar
  2. Asch A, Simerly C, Ord T, Ord VA, Schatten G (1995) The stage at which human fertilization arrests: microtubule and chromosome configurations in inseminated oocytes which failed to complete fertilization and development in humans. Hum Reprod 10:1897–1906PubMedGoogle Scholar
  3. Bahat A, Eisenbach M (2006) Sperm thermotaxis. Mol Cell Endocrinol 252:115–119PubMedGoogle Scholar
  4. Barone JG, De Lara J, Cummings KB, Ward WS (1994) DNA organization in human spermatozoa. J Androl 15:139–144PubMedGoogle Scholar
  5. Barratt CLR, Cooke ID (1988) Sperm loss in the urine of sexually rested men. Int J Androl 11:201–207PubMedGoogle Scholar
  6. Bastiaan HS, Windt ML, Menkveld R, Kruger TF, Oehninger S, Franken DR (2003) Relationship between zona pellucida-induced acrosome reaction, sperm morphology, sperm-zona pellucida binding, and in vitro fertilization. Fertil Steril 79:49–55PubMedGoogle Scholar
  7. Bedford JM (1988) The bearing of epididymal function in strategies for In vitro fertilization and gamete intrafollicular transfer. New York Acad Sci USA 541:284–291Google Scholar
  8. Bedford JM (1990) Sperm dynamics in the epididymis. In: Asch RA, Balmaceda JP, Johnston I (eds) Gamete physiology. Serono Symposia, Norwell, MA, pp 53–67Google Scholar
  9. Bedford JM (1994) The status and the state of the human epididymis. Hum Reprod 9:2187–2199PubMedGoogle Scholar
  10. Bedford JM (1998) Mammalian fertilization misread? Sperm penetration of the eutherian zona pellucida is unlikely to be a lytic event. Biol Reprod 59:1275–1287PubMedGoogle Scholar
  11. Bedford JM, Kim HH (1993) Sperm/egg binding patterns and oocyte cytology in retrospective analysis of fertilization in vitro. Hum Reprod 8:453–463PubMedGoogle Scholar
  12. Bedford JM, Calvin H, Cooper GW (1973) The maturation of spermatozoa in the human epididymis. J Reprod Fertil Suppl 18:199–213PubMedGoogle Scholar
  13. Benoff S, Hurley IR, Mandel FS, Cooper GW, Hershlag A (1997) Induction of the human sperm acrosome reaction with mannose-containing neoglycoproteins. Mol Hum Reprod 3:827–837PubMedGoogle Scholar
  14. Boué F, Sullivan R (1996) Cases of human infertility are associated with the absence of P34H, an epididymal sperm antigen. Biol Reprod 54:1018–1024PubMedGoogle Scholar
  15. Boué F, Duquenne C, Lassalle B, Lefèvre A, Finaz C (1995) FLB1, a human protein of epididymal origin that is involved in the sperm-oocyte recognition process. Biol Reprod 52:267–278PubMedGoogle Scholar
  16. Boué F, Blais J, Sullivan R (1996) Surface localization of P34H, an epididymal; protein, during maturation, capacitation, and acrosome reaction of human spermatozoa. Biol Reprod 54:1009–1017PubMedGoogle Scholar
  17. Brandelli A, Miranda P V, Tezón JG (1996) Voltage-dependent calcium channels and Gi regulatory protein mediate the human sperm acrosomal exocytosis induced by N-acetylglucosaminyl/ mannosyl neoglycoprotein. J Androl 17:522–529PubMedGoogle Scholar
  18. Breitbart H, Cohen G, Rubinstein S (2005) Role of actin cytoskeleton in mammalian sperm capacitation and the acrosome reaction. Reproduction 129:263–268PubMedGoogle Scholar
  19. Brown DB, Hayes EJ, Uchida T, Nagamani M (1995) Some cases of human male infertility are explained by abnormal in vitro human sperm activation. Fertil Steril 64:612–622PubMedGoogle Scholar
  20. Buffet C, Patrat C, Merlet F, Guibert J, Epelboin S, Thiounn N, Vieillefond A, Adda-Lievin A, Lebon C, Jouannet P (2006) ICSI outcomes in obstructive azoospermia: influence of the origin of surgically retrieved spermatozoa and the cause of obstruction. Hum Reprod 21:1018–1024Google Scholar
  21. Carr DW, Newell AE (2007) The role of A-kinase anchoring proteins (AKaps) in regulating sperm function. Soc Reprod Fertil Suppl 63:135–141PubMedGoogle Scholar
  22. Carr DW, Usselman MC, Acott TS (1985) Effects of pH, lactate, and viscoelastic drag on sperm motility: a species comparison. Biol Reprod 33:588–595PubMedGoogle Scholar
  23. Carra E, Sangiorgi D, Gattuccio F, Rinaldi AM (2004) Male infertility and mitochondrial DNA. Biochem Biophys Res Commun 322:333–339PubMedGoogle Scholar
  24. Chakrabarti R, Cheng L, Puri P, Soler D, Vijayaraghavan S (2007) Protein phosphatase PP1 gamma 2 in sperm morphogenesis and epididymal initiation of sperm motility. Asian J Androl 9:445–452PubMedGoogle Scholar
  25. Chemes EH, Rawe YV (2003) Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum Reprod Update 9:405–428PubMedGoogle Scholar
  26. Cooper TG (1993) The human epididymis — is it necessary? Int J Androl 16:245–250PubMedGoogle Scholar
  27. Cooper TG (1995a) The epididymal influence on sperm maturation. Reprod Med Rev 4:141–161Google Scholar
  28. Cooper TG (1995b) Epididymis: role and importance in male infertility treatment. In: Hamamah S, Mieusset R, Dacheux J-L (eds) Frontiers in endocrinology. Ares Serono Symposia, RomeGoogle Scholar
  29. Cooper TG (1996) Physiology and evaluation of epididymal function. In: Comhaire F (ed) Male infertility. Clinical investigation, cause evaluation and treatment. Chapman & Hall, London, pp 97–119Google Scholar
  30. Cooper TG (1998) Epididymis. In: Neill JD, Knobil E (eds) Encyclopedia of reproduction. Academic, San Diego,CA, pp 1–17Google Scholar
  31. Cooper TG (2002) Recent advances in sperm maturation in the human epididymis. Andrologie 12:38–51Google Scholar
  32. Cooper TG (2007a) Sperm maturation in the epididymis: a new look at an old problem. Asian J Androl 9:533–539PubMedGoogle Scholar
  33. Cooper TG (2007b) The human epididymis, sperm maturation and storage. ANIR-ANHP 9:18–21Google Scholar
  34. Cooper TG, Jockenhovel F, Nieschlag E (1991) Variations in semen parameters from fathers. Hum Reprod 6:859–866PubMedGoogle Scholar
  35. Cooper TG, Keck C, Oberdieck U, Nieschlag E (1993) Effects of multiple ejaculations after extended periods of sexual abstinence on total, motile and normal sperm numbers, as well as accessory gland secretions, from healthy normal and oligozoospermic men. Hum Reprod 8:1251–1258PubMedGoogle Scholar
  36. Cooper TG, Barfield JP, Yeung CH (2005) Changes in osmolal-ity during liquefaction of human semen. Int J Androl 28:58–60PubMedGoogle Scholar
  37. Correa-Perez JR, Fernandez-Pelegrina R, Aslanis P, Zavos PM (2004) Clinical management of men producing ejaculates characterized by high levels of dead sperm and altered seminal plasma factors consistent with epididymal necrospermia. Fertil Steril 81:1148–1150PubMedGoogle Scholar
  38. Cross NL (2003) Decrease in order of human sperm lipids during capacitation. Biol Reprod 69:529–534PubMedGoogle Scholar
  39. Cyr DG, Gregory M, Dube E. Dufresne J, Chan PT, Hermo L (2007) Orchestration of occludins, claudins, catenins and cadherins as players involved in mainteance of the blood-epididymal barrier in animals and humans. Asian J Androl 9:463–475PubMedGoogle Scholar
  40. Dacheux JL, Belghazi M, Lanson Y, Dacheux F (2006) Human epididymal secretome and proteome. Mol Cell Endocrinol 250:36–42PubMedGoogle Scholar
  41. Dandekar P, Aggeler J, Talbot P (1992) Structure, distribution and composition of the extracellular matrix of human oocytes and cumulus masses. Hum Reprod 7:391–398PubMedGoogle Scholar
  42. De Jonge C, LaFromboise M, Bosmans E, Ombelet W, Cox A, Nijs M (2004) Influence of the abstinence period on human sperm quality. Fertil Steril 82:57–65PubMedGoogle Scholar
  43. De Kretser DM, Huidobro C, Southwick GJ, Temple-Smith PD (1998) The role of the epididymis in human infertility. J Reprod Fertil Suppl 53:271–275PubMedGoogle Scholar
  44. Delpech S, Lecomte P, Lecomte C (1988) Etude in vitro chez l'homme de la liason des spermatozoies epididymaires a la zone pellucida. J Gynecol Obstet Biol Reprod 17:339–342Google Scholar
  45. Djakiew D, Cardullo R (1986) Lower temperature of the cauda epididymidis facilitates the storage of sperm by enhancing oxygen availability. Gamete Res 15:237–245Google Scholar
  46. Dozortsev D, Rybouchkin A, De Sutter P, Dhont M (1995) Sperm plasma membrane damage prior to intracytoplasmic sperm injection: a necessary condition for sperm nucleus decondensation. Hum Reprod 10:2960–2964PubMedGoogle Scholar
  47. Dozortesev D, Neme R, Diamond MP, Abdelmassih S, Abdelmassih V, Oliveira F, Abdelmassih R (2006) Embryos generated using testicular spermatozoa have higher developmental potential than those obtained using epididymal spermatozoa in men with obstructive azoospermia. Fertil Steril 86:606–611Google Scholar
  48. Dubé E, Chan PT, Hermo L, Cyr DG (2007) Gene expression profiling and its relevance to the blood-epididymal barrier in the human epididymis. Biol Reprod 76:1034–1044PubMedGoogle Scholar
  49. Dubé E, Hermo L, Chan PT, Cyr DG (2008) Alterations in gene expression in the caput epididymides of nonobstructive azoospermic men. Biol Reprod 78:342–351PubMedGoogle Scholar
  50. Edirisinghe WR, Junk SM, Matson PL, Yovich JL (1996) Changes in motility patterns during in vitro culture of fresh and frozen/thawed testicular and epididymal sperm: implications for planning treatment by intracytoplasmic sperm injection. Hum Reprod 11:2474–2476PubMedGoogle Scholar
  51. Eisenbach M, Giojalas LC (2006) Sperm guidance in mammals – an unpaved road to the egg. Nat Rev Mol Cell Biol 7:103–115Google Scholar
  52. Esterhuizen AD, Franken DR, Lourens JG, Lourens JG, Van Rooyen LH (2001) Clinical importance of a micro-assay for the evaluation of sperm acrosome reaction using homologous zona pellucida. Andrologia 33:87–93PubMedGoogle Scholar
  53. Evans EA, Zhang H, Martin-DeLeon PA (2003) SPAM1 (PH-20) protein and mRNA expression in the epididymides of humans and macaques: utilising laser microdissection/ RTPCR. Reprod Biol Endocrinol 1:54–65PubMedGoogle Scholar
  54. Flori F, Ermini L, La Sala GB, Nicoli A, Capone A, Focarelli R, Rosati F, Giovampaola CD (2008) The GPI-anchored CD52 antigen of the sperm surface interacts with semenogelin and participates in clot formation and liquefaction of human semen. Mol Reprod Dev 75:326–335PubMedGoogle Scholar
  55. Franken DR, Bastiaan HS, Oehninger SC (2000) Physiological induction of the acrosome reaction in human sperm: validation of a microassay using minimal volumes of solubilized, homologous zona pellucida. J Assist Reprod Genet 17:156–161PubMedGoogle Scholar
  56. Gadella BM, Harrison RA (2002) Capacitation induces cyclic adenosine 3′, 5′-monophosphate-dependent, but apoptosis-unrelated, exposure of aminophospholipids at the apical head plasma membrane of boar sperm cells. Biol Reprod 67:340–350PubMedGoogle Scholar
  57. Golan R, Cooper TG, Oschry Y, Oberpenning F, Schulze H, Shochat L, Lewin LM (1996) Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry. Hum Reprod 11:1457–1462PubMedGoogle Scholar
  58. Green DP (1997) Three-dimensional structure of the zona pellucida. Rev Reprod 2:147–156.PubMedGoogle Scholar
  59. Haidl G, Opper C (1997) Changes in lipids and membrane anisotropy in human spermatozoa during epididymal maturation. Hum Reprod 12:2720–2723PubMedGoogle Scholar
  60. Haidl G, Badura B, Schill W-B (1994) Function of human epididymal spermatozoa. J Androl 15:23S–27SPubMedGoogle Scholar
  61. Harper CV, Barratt CL, Publicover SJ (2004) Stimulation of human spermatozoa with progesterone gradients to simulate approach to the oocyte. Induction of [Ca2+] (i) oscillations and cyclical transitions in flagellar beating. J Biol Chem 279:46315–46325PubMedGoogle Scholar
  62. Harrison RA, Gadella BM (2005) Bicarbonate-induced membrane processing in sperm capacitation. Theriogenology 63:342–351PubMedGoogle Scholar
  63. Hirsch A, Montgomery J, Mohan P, Mills C, Bekir J, Tan SL (1993) Fertilization by testicular sperm with standard IVF techniques. Lancet 342:1237–1238Google Scholar
  64. Hirsch AV, Dean NL, Mohan PJ, Shaker AG, Bekir JS (1996) Natural spermatocoeles in irreversible obstructive azoo-spermia-reservoirs of viable spermatozoa for assisted conception. Hum Reprod 11:1919–1922Google Scholar
  65. Hoshi K, Tsukikawa S, Sato A (1991) Importance of Ca2+, K+ and glucose in the medium for sperm penetration through human zona pellucida. Tokoku J Exp Med 165:99–104Google Scholar
  66. Inoue N, Ikawa M, Isotani A, Okabe M (2005) The immuno-globulin super family protein Izumo is required for sperm to fuse with eggs. Nature 434:234–238PubMedGoogle Scholar
  67. Jeyendran RS, van der Ven HH, Rosecrans R, Perez-Pelaez M, al-Hasani S, Zaneveld LJ (1989) Chemical constituents of human seminal plasma: relationship to fertility. Andrologia 21:423–428PubMedGoogle Scholar
  68. Jimenez-Gonzalez C, Michelangeli F, Harper CV, Barratt CL, Publicover SJ (2006) Calcium signalling in human spermatozoa: a specialized ‘ toolkit’ of channels, transporters and stores. Hum Reprod Update 12:253–267PubMedGoogle Scholar
  69. Johnson L, Varner DD (1988) Effect of daily sperm production but not age on transit time of spermatozoa through the human epididymis. Biol Reprod 39:812–817PubMedGoogle Scholar
  70. Jones R, James PS, Howes L, Bruckbauer A, Klenerman D (2007) Supramolecular organization of the sperm plasma membrane during maturation and capacitation. Asian J Androl 9:438–444PubMedGoogle Scholar
  71. Jonsson M, Linse S, Forhm B, Lundwall A, Malm J (2005) Semenogelins I and II bind zinc and regulate the activity of prostate-specific antigen. Biochem J 387:47–453Google Scholar
  72. Jow WW, Steckel J, Schlegel PN, Magid MS, Goldstein M (1993) Motile sperm in human testis biopsy specimens. J Androl 14:194–198PubMedGoogle Scholar
  73. Katz DF, Morales P, Samuels SJ, Overstreet JW (1990) Mechanisms of filtration of morphologically abnormal human sperm by cervical mucus. Fertil Steril 54:513–503PubMedGoogle Scholar
  74. Kim YH, Haidl G, Schaefer M, Egner U, Mandal A, Herr JC (2007) Compartmentalization of a unique ADP/ATP carrier protein SFED (sperm flagellar energy carrier, AAC4) with glycolytic enzymes in the fibrous sheath of the human sperm flagellar principal piece. Dev Biol 302:463–476PubMedGoogle Scholar
  75. Kirchhoff C (1995) Molecular biology or sperm maturation in the human epididymis. Reprod Med Rev 4:121–139Google Scholar
  76. Kirchhoff C (2007) Human epididymis — specific gene expression. ANIR 9:25–42Google Scholar
  77. Kishimoto T (2005) Developmental biology: cell cycle unleashed. Nature 437:1048–1052Google Scholar
  78. Kunz G, Beil D, Huppert P, Leyendecker G (2007) Oxytocin-a stimulator of directed sperm transport in humans. Reprod Biomed Online 14:32–39PubMedGoogle Scholar
  79. Lacham-Kaplan O, Trounson A (1997) Fertilization and embryonic development capacity of epididymal and testicular sperm and immature spermatids and spermatocytes. Reprod Med Rev 6:55–68Google Scholar
  80. Lefievre L, Conner S J, Salpekar A, Olufowobi O, Ashton P, Pavlovic B, Lenton W, Afnan M, Brewis IA, Monk M, Hughes DC, Barratt CL (2004) Four zona pellucida glycoproteins are expressed in the human. Hum Reprod 19:1580–1586PubMedGoogle Scholar
  81. Légaré C, Gaudreault C, St-Jacques S, Sullivan R (1999) P34H sperm protein is preferentially expressed by the human corpus epididymidis. Endocrinology 140:3318–3327PubMedGoogle Scholar
  82. Légaré C, Thabet M, Sullivan R (2004) Expression of heat shock protein 70 in normal and cryptorchid human excurrent duct. Mol Hum Reprod 10:197–202PubMedGoogle Scholar
  83. Levitas E, Lunenfeld E, Weiss N, Friger M, Har-Vardi I, Koifman A, Potashnik G (2005) Relationship between duration of sexual abstinence and semen quality; analysis of 9,489 semen samples. Fertil Steril 83:1680–1686PubMedGoogle Scholar
  84. Li JY, Wang H Y, Liu J, Liu Q, Zhang JS, Wan FC, Liu FJ, Jin SH, Zhang YL (2008) Transcriptome analysis of a cDNA library from adult human epididymis. DNA Res 15:115–122PubMedGoogle Scholar
  85. Lin Y, Mahan K, Lathrop WF, Myles DG, Primakoff P (1994) A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg. J Cell Biol 125:1157–1163PubMedGoogle Scholar
  86. Lindemann CB (2007) The geometric clutch as a working hypothesis for future research on cilia and flagella. Ann New York Acad Sci 1101:477–493Google Scholar
  87. Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egestan A (2008) The antibacterial chemokine MIG/CXCL9 s con-stitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma. J Interferon Cytokine Res 28:191–196PubMedGoogle Scholar
  88. Liu DY, Baker HW (1994) Disordered acrosome reaction of spermatozoa bound to the zona pellucida: a newly discovered sperm defect causing infertility with reduced sperm-zona pellucida penetration and reduced fertilization in vitro. Hum Reprod 9:1694–1700PubMedGoogle Scholar
  89. Liu DY, Baker HW (2003a) Disordered zona pellucida-induced acrosome reaction and failure of in vitro fertilization in patients with unexplained infertility. Fertil Steril 79:74–80PubMedGoogle Scholar
  90. Liu DY, Baker HW (2003b) Frequency of defective sperm-zona pellucida interaction in severely teratozoospermic infertile men. Hum Reprod 18:802–807PubMedGoogle Scholar
  91. Liu DY, Baker HW (2004) High frequency of defective sperm— zona pellucida interaction in oligozoospermic infertile men. Hum Reprod 19:228–233PubMedGoogle Scholar
  92. Liu DY, Martic M, Clarke GN, Grkovic I, Garrett C, Dunlop ME, Baker HW (2002) An anti-actin monoclonal antibody inhibits the zona pellucida-induced acrosome reaction and hyperactivated motility of human sperm. Mol Hum Reprod 8:37–47PubMedGoogle Scholar
  93. Liu DY, Clarke GN, Baker HW (2005) Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding. Hum Reprod 20:999–1005PubMedGoogle Scholar
  94. Liu DY, Liu ML, Clarke GN, Baker HW (2007a) Hyperactivation of capacitated human sperm correlates with the zona pellu-cida-induced acrosome reaction of zona pellucida-bound sperm. Hum Reprod 22:2632–2638PubMedGoogle Scholar
  95. Liu DY, Liu ML, Garrett C, Baker HW (2007b) Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen. Hum Reprod 22:1878–1884PubMedGoogle Scholar
  96. Liu J, Tsai YL, Katz E, Compton G, Garcia JE, Baramki TA (1997) Outcome of in-vitro culture of fresh and frozen-thawed human testicular spermatozoa. Hum Reprod 12:1667–1672PubMedGoogle Scholar
  97. Marchetti C, Jouy N, Leroy-Martin B, Formstecher P, Marchetti P (2004) Comparison of four fluorochromes for the detection of the inner mitochondrial membrane potential in human spermatozoa and their correlation with sperm motility. Hum Reprod 19:2267–2276PubMedGoogle Scholar
  98. Mieusset R (1995) Spermatozoa and embryonic development. Front Endocrinol 11:105–128Google Scholar
  99. Mitchell LA, Nixon B, Baker MA, Aitken RJ (2008) Investigation of the role of SRC in capacitation associated tyrosine phos-phorylation of human spermatozoa. Mol Hum Reprod 14:235–243PubMedGoogle Scholar
  100. Moghadam KK, Nett R, Robins JC, Thomas MA, Awadalla SG, Scheiber MD, Williams DB (2005) The motility of epididymal or testicular spermatozoa does not directly affect IVF/ICSI pregnancy outcomes. J Androl 26:619–623PubMedGoogle Scholar
  101. Montag M, Tok V, Liow S-L, Bongso A, Ng SC (1992) In vitro decondensation of mammalian sperm and subsequent formation of pronuclei-like structures for micromanipulation. Mol Reprod Dev 33:338–346PubMedGoogle Scholar
  102. Moore HDM, Hartmann TD, Pryor JP (1983) Development of the oocyte-penetrating capacity of spermatozoa in the human epididymis. Int J Androl 6:310–318PubMedGoogle Scholar
  103. Moore HDM, Curry MR, Penfold LM, Pryor JP (1992) The culture of human epididymal epithelium and in vitro maturation of epididymal spermatozoa. Fertil Steril 58:776–783PubMedGoogle Scholar
  104. Mortimer D (1995) In: Grudzinskas JG, Yovich JL (eds) Gametes. The spermatozoon. Cambridge University Press, Cambridge, pp 157–174Google Scholar
  105. Mortimer ST, Swan MA (1995) Variable kinematics of capacitating human spermatozoa. Hum Reprod 10:3178–3182PubMedGoogle Scholar
  106. Moskovtsev SI, Jarvi K, Légaré C, Sullivan R, Mullen JB (2007) Epididymal P34H protein deficiency in men evaluated for infertility. Fertil Steril 88:1455–1457PubMedGoogle Scholar
  107. Navarra CS, Simerly C, Zoran S, Schatten G (1995) The sperm centrosome during fertilization in mammals: implications for fertility and reproduction. Reprod Fertil Develop 7:747–754Google Scholar
  108. Neugebauer DC, Neuwinger J, Jockenhövel F, Nieschlag E (1990) ‘ 9 + 0’ axoneme in spermatozoa and some nasal cilia of a patient with totally immotile spermatozoa associated with thickened sheath and short midpiece. Hum Reprod 5:981–986PubMedGoogle Scholar
  109. Nixon B, Aitken RJ, McLaughlin EA (2007) New insights into the molecular mechanisms of sperm-egg interaction. Cell Mol Life Sci 64:1805–1823PubMedGoogle Scholar
  110. Overstreet JW, Hembree WC (1976) Penetration of the zona pel-lucida of nonliving human oocytes by human spermatozoa in vitro. Fertil Steril 27:815–831PubMedGoogle Scholar
  111. Palermo GD, Avrech OM, Colombero LT, Wu H, Wolny YM, Fissore RA, Rosenwaks Z (1997) Human sperm cytosolic factor triggers Ca2+ oscillations and overcomes activation failure of mammalian oocytes. Mol Hum Reprod 3:367–337PubMedGoogle Scholar
  112. Patrizio P, Ord T, Silber SJ, Asch RH (1994) Correlation between epididymal length and fertilization rate in men with congenital absence of the vas deferens. Fertil Steril 61:265–268PubMedGoogle Scholar
  113. Phillips DM (1991) Structure and function of the zona pellucida. In: Familiari G, Makabern S, Motta PM (eds) Ultrastructure of the ovary. Kluwer, Den Haag, The Netherlands, pp 63–72Google Scholar
  114. Pöllänen P, Cooper TG (1994) Immunology of the testicular excurrent ducts. J Reprod Immunol 26:167–216PubMedGoogle Scholar
  115. Pöllänen P, Cooper TG, Yeung CH, Saari T (1995) Regulation of the transport of immunoglobulin G into the male rat reproductive tract. J Reprod Immunol 28:111–135PubMedGoogle Scholar
  116. Potts RJ, Jefferies TM, Notarianni LJ (1999) Antioxidant capacity of the epididymis. Hum Reprod 14:2513–2516PubMedGoogle Scholar
  117. Primakoff P, Myles DG (2007) Cell-cell membrane fusion during mammalian fertilization. FEBS Lett 581:2174–2180PubMedGoogle Scholar
  118. Qi H, Moran MM, Navarro B, Chong JA, Krapivinsky G, Krapivinsky L, Kirichok Y, Ramsey IS, Quill TA, Clapham DE (2007) All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proc Nat Acad Sci USA 104:1107–1108Google Scholar
  119. Reyes-Moreno C, Laflamme J, Frenette G, Sirard MA, Sullivan R (2008) Spermatozoa modulate epididymal cell proliferation and protein secretion in vitro. Mol Reprod Dev 75:512–520PubMedGoogle Scholar
  120. Rhouma KB, Miled EB, Attallah K, Marrakchi H, Khouja H, Sakly M (2003) Successful pregnancies after using immotile spermatozoa from ejaculate, epididymis and testis. Eur J Obstet Gynecol Reprod Biol 108:182–185PubMedGoogle Scholar
  121. Sathananthan AH, Ratnam SS, Ng SC, Tarín JJ, Gianaroli L, Trounson A (1996) The sperm centriole: its inheritance, replication and perpetuation in early human embryos. Hum Reprod 11:345–356PubMedGoogle Scholar
  122. Schultz RM (2002) The molecular foundations of the maternal to zygotic transition in the preimplantation embryo. Hum Reprod Update 8:323–331PubMedGoogle Scholar
  123. Shadan S, James PS, Howes EAJR (2004) Cholesterol efflux alters lipid raft stability and distribution during capacitation of boar spermatozoa. Biol Reprod 71:253–265PubMedGoogle Scholar
  124. Shefi S, Raviv G, Eisenberg ML, Weissenberg R, Jalalian L, Levron J, Band G, Turek PJ, Madgar I (2006) Posthumous sperm retrieval analysis of time interval to harvest sperm. Hum Reprod 21:2890–2893PubMedGoogle Scholar
  125. Shur BD, Rodeheffer C, Ensslin MA, Lyng R, Raymond A (2006) Identification of novel gamete receptors that mediate sperm adhesion to the egg coat. Mol Cell Endocrinol 250:137–148PubMedGoogle Scholar
  126. Sobrero AJ, MacLeod J (1962) The immediate postcoital test. Fertil Steril 13:184–189PubMedGoogle Scholar
  127. Soler C, Pérez-Sánchez F, Schulze H, Bergmann M, Oberpenning F, Yeung C, Cooper TG (2000) Objective evaluation of the morphology of human epididymal sperm heads. Int J Androl 23:77–84PubMedGoogle Scholar
  128. Sousa M, Mendoza C, Barros A, Tesarik J (1996) Calcium responses of human oocytes after intracytoplasmic injection of leukocytes, spermatocytes and round spermatids. Mol Hum Reprod 2:853–857PubMedGoogle Scholar
  129. Spehr M, Schwane K, Riffell JA, Zimmer RK, Hatt H (2006) Odorant receptors and olfactory-like signaling mechanisms in mammalian sperm. Mol Cell Endocrinol 250:128–136PubMedGoogle Scholar
  130. Stalf T, Mehnert C, Hajimohammad A, Manolopoulos K, Shen Y, Schuppe HC, Diemer T, Schill WB, Weidner W, Tinneberg HR (2005) Influence of motility and vitality in intracytoplas-mic sperm injection with ejaculated and testicular sperm. Andrologia 37:125–130PubMedGoogle Scholar
  131. Suarez SS, Pacey AA (2006) Sperm transport in the female reproductive tract. Hum Reprod Update 12:23–37PubMedGoogle Scholar
  132. Sullivan R, Légaré C, Villeneuve M, Foliguet B, Bissonnette F (2006) Levels of P34H, a sperm protein of epididymal origin, as a predictor of conventional in vitro fertilization outcome. Fertil Steril 85:1557–1559PubMedGoogle Scholar
  133. Sullivan R, Légaré C, Thimon V (2007) Effects of vasectomy on protein secretion and sperm maturation along the human epididymis. ANIR 9:43–48Google Scholar
  134. Sun F, Bahat A, Gakamsky A, Girsh E, Katz N, Giojalas LC, Tur-Kaspa I, Eisenbach M (2005) Human sperm chemot-axis: both the oocyte and its surrounding cumulus cells secrete sperm chemoattractants. Hum Reprod 20:761–767PubMedGoogle Scholar
  135. Tesarik J, Mendoza C (1992) Defective function of a nonge-nomic progesterone receptor as a sole sperm anomaly in infertile patients. Fertil Steril 58:793–797PubMedGoogle Scholar
  136. Tesarik J, Mendoza C (1993) Sperm treatment with pentoxifyl-line improves the fertilizing ability in patients with acrosome reaction insufficiency. Fertil Steril 60:141–148PubMedGoogle Scholar
  137. Tesarik J, Thebault A (1993) Fertilization failure after subzonal sperm insertion associated with defective functional capacity of acrosome-reacted spermatozoa. Fertil Steril 60:369–371PubMedGoogle Scholar
  138. Thimon V, Koukoui O, Calvo E, Sullivan R (2007) Region-specific gene expression profiling along the human epididymis. Mol Hum Reprod 13:691–704PubMedGoogle Scholar
  139. Thimon V, Calvo E, Koukoui O, Légaré C, Sullivan R (2008) Effects of vasectomy on gene expression profiling along the human epididymis. Biol Reprod 79:262–273PubMedGoogle Scholar
  140. Turner T (2008) De Graaf's thread: the human epididymis. J Androl 29:237–250PubMedGoogle Scholar
  141. Van Steirteghem AC, Nagy Z, Liu J, Joris H, Janssenswillen C, Silber S, De Vroey P (1995) Embryo development after ICSI using testicular, epididymal and ejaculated spermatozoa. In: Hamamah S, Mieusset R, Dacheux JL (eds) Frontiers in endocrinology. Epididymis: role and importance in male infertility treatment. Ares Serono Symposia, Rome, pp 141–147Google Scholar
  142. Visconti PE, Galantino-Homer H, Moore GD, Bailey JL, Ning X, Fornes M, Kopf GS (1998) The molecular basis of sperm capacitation. J Androl 19:242–248PubMedGoogle Scholar
  143. von Lanz T, Neuhauser G (1964) Morphometrische Analyse des menschlichen Nebenhodens. Z f Anat Entwickl 124:126–152Google Scholar
  144. Wassarman P (1992) Regulation of mammalian fertilization by gamete adhesion molecules. In: Nieschlag E, Habenicht UA (eds) Spermatogenesis, fertilization, contraception. Molecular, cellular and endocrine events in male reproduction. Springer, Berlin, pp 345–366Google Scholar
  145. Williams CJ (2002) Signalling mechanisms of mammalian oocyte activation. Hum Reprod Update 8:313–321PubMedGoogle Scholar
  146. Williamson K, Frayne J, McLaughlin EA, Hall L (1998) Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein. Mol Hum Reprod 4:235–242Google Scholar
  147. Yanagimachi R (1994) Mammalian fertilization. In: Knobil E, Neill JD (eds) The physiology of reproduction, 2nd edn. Raven, New York, pp 189–317Google Scholar
  148. Yeung CH (1995) Development of sperm infertility. In: Hamamah S, Mieusset R, Dacheux JL (eds) Frontiers in endocrinology. Epididymis: role and importance in male infertility treatment. Ares Serono Symposia, Rome, pp 73–86Google Scholar
  149. Yeung CH, Cooper TG (2001) Effects of the ion-channel blocker quinine on human sperm volume, kinematics and mucus penetration, and the involvement of potassium channels. Mol Human Reprod 7:819–828Google Scholar
  150. Yeung CH, Cooper TG (2002) Acquisition and development of sperm motility upon maturation in the epididymis. In: Robaire B, Hinton BT (ed) The epididymis: from molecules to clinical practice. A comprehensive survey of the efferent ducts, the epididymis and the vas deferens. Kluwer/Plenum, New YorkGoogle Scholar
  151. Yeung CH, Bals-Pratsch M, Knuth UA, Nieschlag E (1988) Investigation of the cause of low sperm motility in astheno-zoospermic patients by multiple quantitative tests. Int J Androl 11:289–299PubMedGoogle Scholar
  152. Yeung CH, Cooper TG, Weinbauer GF, Bergmann M, Kleinhans G, Schulze H, Nieschlag E (1989) Fluid-phase transcytosis in the primate epididymis in vitro and in vivo. Int J Androl 12:384–394PubMedGoogle Scholar
  153. Yeung CH, Cooper TG, Bergmann M, Schulze H (1991) Organization of tubules in the human caput epididymidis and the ultrastructure of their epithelia. Am J Anat 191:261–279PubMedGoogle Scholar
  154. Yeung CH, Cooper TG, Oberpenning F, Schulze H, Nieschlag E (1993) Changes in movement characteristics of human spermatozoa along the length of the epididymis. Biol Reprod 49:274–280PubMedGoogle Scholar
  155. Yeung CH, Nashan D, Sorg C, Oberpenning F, Schulze H, Nieschlag E, Cooper TG (1994) Basal cells of the human epididymis – antigenic and ultrastructural similarities to tis-sue-fixed macrophages. Biol Reprod 50:917–926PubMedGoogle Scholar
  156. Yeung CH, Cooper TG, Majumder GC, Rolf C, Behre H (1996) The role of phosphocreatine kinase in the motility of human spermatozoa supported by different metabolic substrates. Mol Hum Reprod 2:591–596PubMedGoogle Scholar
  157. Yeung CH, Perez-Sanchez F, Soler C, Poser D, Kliesch S, Cooper TG (1997) Maturation of human spermatozoa (from selected epididymides of prostatic carcinoma patients) with respect to their morphology and ability to undergo the acrosome reaction. Hum Reprod Update 3:205–213PubMedGoogle Scholar
  158. Yoshida K, Kawano N, Yoshiike M, Yoshida M, Iwamato T, Morisawa M (2008) Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans. Mol Human Reprod 14:151–156Google Scholar
  159. Zamboni L (1992) Sperm structure and its relevance to infertility. An electron microscopic study. Arch Pathol Lab Med 116:325–344PubMedGoogle Scholar
  160. Zarintosh RJ, Cross NL (1996) Unesterified cholesterol content of human sperm regulates the response of the acrosome to the agonist, progesterone. Biol Reprod 55:19–24Google Scholar
  161. Zenke U, Jalalian L, Shen S, Turek PJ (2004) The difficult MESA: findings from tubuli recti sperm aspiration. J Assist Reprod Genet 21:31–35PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Centre of Reproductive Medicine and Andrology of the UniversityMünsterGermany
  2. 2.Centre of Reproductive Medicine and Andrology of the UniversityMünsterGermany

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