Abstract
Endolithic bioerosion is difficult to analyse and to describe, and it usually requires damaging of the sample material. Sponge erosion (Entobia) may be one of the most difficult to evaluate, as it is simultaneously macroscopically inhomogeneous and microstructurally intricate. We studied the bioerosion traces of the two Australian sponges Cliona celataand Cliona orientaliswith modern technology: high resolution X-ray micro-computed tomography. Micro-CT allows non-destructive visualisation of live and dead structures in three dimensions and was compared to traditional microscopic methods. Micro-CT and microscopy showed that C. celatabioerosion was more intense in the centre and branched out in the periphery (21 vs. 9% substrate removed). In contrast, C. orientalisproduced a dense, even meshwork and caused an overall more intense erosion pattern than C. celata(48 central vs. 42% marginal substrate removed). Extended pioneering filaments were not usually found at the margins of the studied sponge erosion, but branches ended abruptly or tapered to points. Results obtained with micro-CT were similar in quality to observations from transparent optical spar under the dissecting microscope. Microstructures could not be resolved as well with micro-CT as anticipated. Even though sponge scars and sponge chips were easily recognisable on maximum magnification micro-CT images, they lacked the detail that is available from SEM. Other drawbacks of micro-CT involve high costs and presently limited access. Even though micro-CT cannot presently replace traditional techniques such as epoxy resin casts viewed by SEM, we obtained valuable information. Especially for the possibility to measure endolithic pore volumes, we regard micro-CT as a very promising tool that will continue to be optimised. A combination of different methods will produce the best results in the study of Entobia
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References
Becker LC, Reaka-Kudla ML (1997) The use of tomography in assessing bioerosion in corals. Proc 8th Int Coral Reef Symp, Smithson Trop Res Inst, Panama 1996, 2:1819-1824
Bergman KM (1983) The distribution and ecological significance of the boring sponge Cliona viridison the Great Barrier Reef, Australia. MSc Thesis, Geol Dept McMaster Univ, Hamilton / Canada, 69 pp
Beuck L, Vertino A, Stepina E, Karolczak M, Pfannkuche O (2007) Skeletal response of Lophelia pertusa(Scleractinia) to bioeroding sponge infestation visualised with microcomputed tomography. Facies 53:157-176
Borchiellini C, Alivon E, Vacelet J (2004) The systematic position of Alectona(Porifera, Demospongiae): a tetractinellid sponge. Boll Mus Ist Univ Genova 68:209-217
Bromley RG, D’Alessandro A (1989) Ichnological study of shallow marine endolithic sponges from the Italian coast. Riv Ital Paleont Stratigr 95:279-314
Bromley RG, Tendal OS (1973) Example of substrate competition and phobotropism between two clionid sponges. J Zool London 169:151-155
Bromley RG, Beuck L, Taddei Ruggiero E (this volume) Endolithic sponge versus terebratulid brachiopod, Pleistocene, Italy: accidental symbiosis, bioclaustration, deformity and death. In: Wisshak M, Tapanila L (eds) Current developments in bioerosion. Springer, Berlin, pp 361-368
Bronn HG (1837) Lethaea Geognostica oder Abbildungen und Beschreibungen der für die Gebirgs-Formationen bezeichnendsten Versteinerungen. Schweizerbart, Stuttgart, 1:1-544 and plates
Calcinai B, Arillo A, Cerrano C, Bavestrello G (2003) Taxonomy-related differences in the excavating micro-patterns of boring sponges. J Mar Biol Assoc UK 83:37-39
Calcinai B, Bavestrello G, Cerrano C (2004) Bioerosion micro-patterns as diagnostic characteristics in boring sponges. Boll Mus Ist Univ Genova 68:229-238
Farber L, Tardos G, Michaels J (2003) The use of X-ray tomography to study the porosity and morphology of granules. Powder Technol 132:57-63
Feldkamp LA, Davis LC, Kress JW (1984) Practical cone-beam algorithm. J Optic Soc Amer, Ser A 1:612-619
Fischer MP (1868) Recherches sur les éponges perforantes fossiles. Nuov Arch Mus Hist Nat Paris 4:117-173
Freudenthal GH (1962) Symbiodinium gen. nov. and Symbiodinium microadriaticumsp. nov., a zooxanthella: taxonomy, life cycle, and morphology. J Protozool 9:45-52
Grant RE (1826) Notice of a new zoophyte (Cliona celata, Gr.) from the Firth of Forth. Edinburgh N Phil J 1:78-81
Hancock A (1849) On the excavating powers of certain sponges belonging to the genus Cliona; with descriptions of several new species, and an allied generic form. Ann Mag Nat Hist, Ser 2, 3:321-348
Hancock A (1867) Note on the excavating sponges; with descriptions of four new species. Ann Mag Nat Hist, Ser 3, 19:229-242
Hoeksema BW (1983) Excavation patterns and spiculae dimensions of the boring sponge Cliona celatafrom the SW Netherlands. Senckenbergiana Marit 15:55-85
Itoh M, Shimazu A, Hirata I, Yoshida Y, Shintani H, Okazaki M (2004) Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography. Biomaterials 25:2577-2583
Johnson JY (1899) Notes on some sponges belonging to the Clionidae obtained at Madeira. J Roy Microscop Soc 9:461-463
Lin C, Miller J (1996) Cone beam X-ray microtomography for three-dimensional liberation analysis in the 21st century. Int J Miner Process 47:61-73
Lindgren NG (1897) Beitrag zur Kenntnis der Spongienfauna des Malayischen Archipels und der chinesischen Meere. Zool Anz 20:486-487
PANGAEA (accessed June 25th, 2007) Publishing network for geoscientific and environmental data. http://www.pangaea.de/
Ramakrishna K, Muralidhar K, Munshi P (2006) Beam-hardening in simulated X-ray tomography. Nondestruct Test Eval Int 39:449-457
Rosell D, Uriz M-J (2002) Excavating and endolithic sponge species (Porifera) from the Mediterranean: species descriptions and identification key. Org Diver Evol 2:55-86
Rützler K (1974) The burrowing sponges of Bermuda. Smithson Contr Zool 165:1-32
Rützler K (1975) The role of burrowing sponges in bioerosion. Oecologia 19:203-216
Schönberg CHL (2000) Bioeroding sponges common to the central Australian Great Barrier Reef: description of three new species, two new records, and additions to two previously described species. Senckenbergiana Marit 30:161-221
Schönberg CHL (2001) Estimating the extent of endolithic tissue of a Great Barrier Reef clionid sponge. Senckenbergiana Marit 31:29-39
Schönberg CHL (2003) Substrate effects on the bioeroding demosponge Cliona orientalis. 2. Substrate colonisation and tissue growth. Publ Stn Zool Napoli Mar Ecol 24:59-74
Schönberg CHL (2006) Growth and erosion of the zooxanthellate Australian bioeroding sponge Cliona orientalisare enhanced in light. In: Suzuki Y, Nakamori T, Hidaka M, Kayanne H, Casareto BE, Nadao K, Yamano H, Tsuchiya M (eds) Proc 10th Int Coral Reef Symp, Okinawa, Japan, pp 166-174
Schönberg CHL, Loh WK (2005) Molecular identity of the unique symbiotic dinoflagellates found in the bioeroding demosponge Cliona orientalis. Mar Ecol Prog Ser 299:157-166
Schönberg CHL, Grass S, Heiermann AT (2006) Cliona minuscula, sp. nov. (Hadromerida: Clionaidae) and other bioeroding sponges that only contain tylostyles. Zootaxa 1312:1-24
Tapanila L (this volume) The medium is the message: imaging a complex microboring (Pyrodendrina cupraigen. n., isp. n.) from the early Paleozoic of Anticosti Island, Canada. In: Wisshak M, Tapanila L (eds) Current developments in bioerosion. Springer, Berlin, pp 123-146
Thiele J (1900) Kieselschwämme von Ternate. 1. Abh Senckenb Natf Ges 25:19-80
Topsent E (1888) Contribution à l’étude des Clionides. Mém Soc Zool France 2:1-165
Topsent E (1905) Cliothosa seurati, Clionide nouvelle des Îles Gambier. Bull Mus Hist Nat Paris 2:94-96
Van Geet M, Swennen R, Wevers M (2001) Towards 3-D petrography: application of microfocus computer tomography in geological science. Computer Geosci 27:1091-1099
Wesche SJ, Adlard RD, Hooper JNA (1997) The first incidence of clionid sponges (Porifera) from the Sydney rock oyster Saccostrea commercialis (Iredale and Roughley, 1933).Aquaculture 157:173-180
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Schönberg, C.H., Shields, G. (2008). Micro-computed tomography for studies on Entobia: transparent substrate versus modern technology. In: Wisshak, M., Tapanila, L. (eds) Current Developments in Bioerosion. Erlangen Earth Conference Series. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-77598-0_8
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