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RNA Interference in Mammalian Cell Systems

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RNA Interference

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 320))

Abstract

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cells has almost become routine laboratory practice. Through refinement of double-stranded RNA (dsRNA) triggers of RNAi and creation of genome-scale libraries, the first genome-wide loss of function screens have been carried out in mammals. This review discusses some of the key features of RNAi in mammalian systems.

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Paddison, P.J. (2008). RNA Interference in Mammalian Cell Systems. In: Paddison, P.J., Vogt, P.K. (eds) RNA Interference. Current Topics in Microbiology and Immunology, vol 320. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-75157-1_1

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