Rhizosphere Metabolomics: Methods and Applications
The emerging field of rhizosphere metabolomics involves analysis of entire metabolite complement (metabolome), in an unbiased way to understand complex physiological, pathological, symbiotic and other relationships among the inhabitants of the rhizosphere. Metabolomic studies of the rhizosphere are quite challenging since the rhizosphere is a complex as well as a dynamic microenvironment. Metabolite composition in the rhizosphere is primarily governed by the nature of root exudates, secretions from rhizobacteria, fungi and other soil organisms. Conversely, the nature of these root exudates also directly or indirectly affects microbial growth in the rhizosphere. While some compounds enhance growth, others have antimicrobial activities. Apart from the diverse roles of compounds present, the complexity of the rhizosphere also stems from competition among rhizosphere microbes. Some of them are growth-promoting, while others are pathogenic. These effects are not only confined to the microbes but also extend to the plants growing in the rhizosphere. Hence, gaining knowledge of these rhizosphere metabolites as well as the effect of the biota will help us better understand this ecological niche.
KeywordsNuclear Magnetic Resonance Root Exudate Nuclear Magnetic Resonance Spectroscopy Metabolomic Study Metabolomics Data
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