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Dual SPR-Impedance Measurement System for detection of bioaffinity interactions

  • S. David
  • M. Gheorghiu
  • Cristina Polonschii
  • E. Gheorghiu
Part of the IFMBE Proceedings book series (IFMBE, volume 17)

Abstract

The capabilities of differential impedance spectroscopy to assess bioaffinity sensors (with the surface modified with high specificity compounds) for both direct and competitive determinations were investigated using a novel Dual Surface Plasmon Resonance (SPR) - Differential Electrical Impedance (DEI) measurement set-up, aiming for development of an effective assay for detection of target analytes. The surface changes due to the specific recognition events are simultaneously monitored, on the surface of the same chip, using this custom designed system that comprises: a 3 channel SPR module, a multi-channel, differential impedance analyzer, a fully automated injection system (with capabilities for manual injecting and programmed protocols) and a 3 micro channels flow through measurement chamber. In this format, the variations of impedance of the sensor/specimen interface in response to fast specific recognition events (Ag-Ab) are revealed, while eliminating the nonspecific influences; simultaneous SPR & DEI measurements provide inner validation and expand the analyte detection range.

Keywords

Surface Plasmon Resonance Sensor Surface Surface Plasmon Resonance Measurement Refractive Index Sensor Surface Plasmon Resonance Module 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    O.A. Sadik, E. Gheorghiu, H. Xu, D. Andreescu, C. Balut, M. Gheorghiu, D. Bratu (2002) Differential Impedance Spectroscopy for Monitoring Protein Immobilization and Antibody-Antigen Reactions Analytical Chemistry 74:3142-3150CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • S. David
    • 1
  • M. Gheorghiu
    • 1
  • Cristina Polonschii
    • 1
  • E. Gheorghiu
    • 1
  1. 1.International Centre of BiodynamicsBucharestRomania

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