Advertisement

Measurements on cultured cells using screen printed sensors

  • M. Brischwein
  • B. Gleich
  • T. Weyh
  • P. Los
Part of the IFMBE Proceedings book series (IFMBE, volume 17)

Abstract

Impedance sensors in thick film technology have been designed as a tool for electric cell impedance sensing. The screen printed gold electrodes and the flexible PET polymeric chip substrate are homogeneously grown with Hela cells as documented by fluorescence microscopy. In contrast to previously published cell impedance measurements, we discuss the use of revised experimental conditions to improve the accuracy of the measurements. Responses of confluent cell monolayers to the treatment with two bioactive compounds are tested: (1) Histamine, triggering a cell morphological reaction and (2) the non-ionic detergent Triton X-100, leading to a complete disruption of cell membranes. The impedance is measured and compared with a standard, planar electrode arrangement for statistical evaluation.

Keywords

Cervix Carcinoma Confluent Cell Monolayer Mechanical Setup Impedance Sensor Sandwich Sheet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Giaever I, Keese C R (1984) Monitoring fibroblast behavior in tissue culture with a applied electric field. Proc Natl Acad Sci 81: 3761-3764CrossRefGoogle Scholar
  2. 2.
    Brischwein M, Herrmann S et al (2006) Electric cell-substrate impedance sensing with screen printed electrode structures. Lab on a Chip 6: 819-822 DOI: 10.1039/B602987F CrossRefGoogle Scholar
  3. 3.
    Motrescu E M, Brischwein M et al (2001) Comparison of Ca2+ sensitive dye probes and microsensor-based monitoring in the assessment of cellular signaling. Biol Cell 93:225Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2007

Authors and Affiliations

  • M. Brischwein
    • 1
  • B. Gleich
    • 1
  • T. Weyh
    • 1
  • P. Los
    • 2
  1. 1.Technische Universität München/Heinz Nixdorf-Lehrstuhl für Medizinische ElektronikMünchenGermany
  2. 2.IDMoS plcDundeeUK

Personalised recommendations