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Molecular Genetics of Recombination pp 469–486Cite as

V(D)J recombination: mechanism and consequences

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Part of the book series: Topics in Current Genetics ((TCG,volume 17))

Abstract

V(D)J recombination is responsible for assembling the functional immunoglobulin and T cell receptor genes in cells of the immune system. This specialized DNA rearrangement is initiated by the RAG1 and RAG2 proteins, lymphoid-specific factors which collaborate to make double-strand breaks at specific sites that flank segments of coding sequence. During breakage, the ends of the coding DNA are converted to DNA hairpins. Joining of the broken ends is then carried out by the non-homologous end-joining pathway, after the hairpin ends are cut open by the Artemis protein. The RAG1/2 complex can also carry out transpositional recombination, a reaction that helps to explain how the RAG proteins work, and supports previous suggestions that V(D)J recombination evolved from a mobile DNA.

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  1. Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 5, Room 241, 9000 Rockville Pike, Bethesda, MD, 20892-0540, USA

    Martin Gellert

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  1. Martin Gellert
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  1. Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, CSIC-Universidad de Sevilla, Av. Américo Vespucio s/n, 41092, Sevilla, Spain

    Andrés Aguilera

  2. Department of Genetics & Development, Columbia University Medical Center, 701 West 168th Street, HHSC 1608, New York, NY, 10032-2704, USA

    Rodney Rothstein

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Gellert, M. (2007). V(D)J recombination: mechanism and consequences. In: Aguilera, A., Rothstein, R. (eds) Molecular Genetics of Recombination. Topics in Current Genetics, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-71021-9_16

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