Abstract
The Polymerase Chain Reaction process is a well-known technique for the in vitro amplification of a DNA sequence. The success of a PCR depends on several parameters particularly the primer sequences used. Since the design of a suitable pair of primer involves a reasonable number of variables, which can have a range of different values, computer programs are commonly used to assist this task. This paper approaches the design of a pair of primer sequences as a search process throughout the space defined by all possible primer sequence pairs, directed by an evaluation function that combines the many variables involved in a primer design; an experiment and its results are discussed.
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Montera, L., Nicoletti, M.C. (2008). The PCR Primer Design as a Metaheuristic Search Process. In: Rutkowski, L., Tadeusiewicz, R., Zadeh, L.A., Zurada, J.M. (eds) Artificial Intelligence and Soft Computing – ICAISC 2008. ICAISC 2008. Lecture Notes in Computer Science(), vol 5097. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-69731-2_91
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DOI: https://doi.org/10.1007/978-3-540-69731-2_91
Publisher Name: Springer, Berlin, Heidelberg
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