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Osteogenesis on Surface Selective Laser Sintered Bioresorbable Scaffolds

  • Victor Bagratashvili
  • E. N. Antonov
  • S. M. Howdle
  • J. M. Kanczler
  • S. Mirmalek-Sani
  • V. K. Popov
  • R. O. Oreffo
  • C. Upton
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 20)

Abstract

In this study we have used a novel surface selective laser sintering (SSLS) technique to develop CAD/CAM designed scaffolds for bone tissue engineering. SSLS polylactic acid scaffolds were evaluated in vitro and in vivo as templates for human fetal femur-derived cell and adult human bone marrow stromal cell osteogenesis. Both cell types were cultured successfully on SSLS scaffolds with an increase in expression of alkaline phosphatase activity. Cell in-growth and Alcian blue/Sirius red positive staining of matrix deposition were observed on SSLS scaffolds in vitro in basal medium and osteogenic culture conditions. Similar results were observed in vivo with type I collagen expressed by cells on the scaffolds. In the critical sized femur segmental defect, SSLS scaffolds seeded with the cells enhanced significantly bone tissue regeneration.

Keywords

Tissue engineering laser sintering scaffolds 

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References

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    Hutmacher D.W., (2000) “Scaffolds in tissue engineering bone and cartilage”, Biomaterials, V. 21, pp.2529–2543.CrossRefGoogle Scholar
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    Antonov E.N., Bagratashvili V.N., Howdle S.M., Konovalov A.N., Popov V.K., Shakesheff K.M. and Whitaker M.J., (2005) “Threedimensional bioactive and biodegradable scaffolds fabricated by surface-selective laser sintering”, Advanced Materials, V. 17, No. 3, 327–330.CrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 2008

Authors and Affiliations

  • Victor Bagratashvili
    • 1
  • E. N. Antonov
    • 1
  • S. M. Howdle
    • 3
  • J. M. Kanczler
    • 2
  • S. Mirmalek-Sani
    • 2
  • V. K. Popov
    • 1
  • R. O. Oreffo
    • 2
  • C. Upton
    • 3
  1. 1.Institute of Laser and Information Technologies RASTroitsk, Moscow RegionRussia
  2. 2.Bone & Joint Research GroupUniversity of SouthamptonSouthampton
  3. 3.School of ChemistryUniversity of NottinghamUniversity Park NottinghamUK

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