Abstract
We are pursuing a hybrid optical-electrophysiological- computational approach for studying and modeling neuronal input-output transformations in CNS neurons in vitro under increasingly realistic conditions. Our approach combines two-photon glutamate uncaging, which enables noninvasive stimulation of neurons with single synapse specificity, and rapid three-dimensional hopping between many different uncaging locations (termed “patterned uncaging”). The input sequences used are doubly stochastic Poisson point processes with predefined custom auto- and cross-correlation functions, generated using a new method we developed.
Our experiments quantitatively analyze the role of the input correlation structure on dendritic integration in CNS neurons by experimentally mimicking natural synaptic activity while simultaneously electrically measuring the output soma potentials.
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References
Hausser, M., and Mel, B. (2003). Dendrites: bug or feature? Current Opinion in Neurobiology 13, 372–383.
Poirazi, P., Brannon, T., and Mel, B. W. (2003). Pyramidal Neuron as Two-Layer Neural Network. Neuron 37, 989–999.
Magee, J. C. (2000). Dendritic Integration of Excitatory Synaptic Input. Nature Reviews Neuroscience 1, 181–190
Matsuzaki, M., Ellis-Davies, G. C. R., Nemoto, T., Miyashita, Y., Iino, M., and Kasai, H. (2001). Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons. Nat Neurosci 4, 1086–1092.
Shoham, S., O’Connor, D. H., Sarkisov, D. V., and Wang, S. S. H. (2005). Rapid neurotransmitter uncaging in spatially defined patterns. Nat Meth 2, 837–843.
Tsai, P. S., Nishimura, N., J., Y. E., Dolnick, E. M., White, G. A., and Kleinfeld, D. (2002). Principles, Design, and Construction of a Two-Photon Laser-Scanning Microscope for In Vitro and In Vivo Brain Imaging. In In Vivo Optical Imaging of Brain Function, R. D. Frostig, ed. (CRC Press), pp. 113–171.
Pologruto, T., Sabatini, B., and Svoboda, K. (2003). ScanImage: Flexible software for operating laser scanning microscopes. BioMedical Engineering OnLine 2, 13.
Niebur, E. (2007). Generation of Synthetic Spike Trains with Defined Pairwise Correlations. Neural Comp 19, 1720–1738.
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Krumin, M., Shoham, S. (2008). Characterization of Input-Output Relations in Single Neurons using Spatiotemporal Photo-stimulation. In: Katashev, A., Dekhtyar, Y., Spigulis, J. (eds) 14th Nordic-Baltic Conference on Biomedical Engineering and Medical Physics. IFMBE Proceedings, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-69367-3_101
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DOI: https://doi.org/10.1007/978-3-540-69367-3_101
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