Abstract
Before introduction of the chromatin spreading technique by Miller and Beatty in 1969 (Miller and Beatty 1969 a-d), relatively limited information was available concerning the submicroscopic organization of chromosomes in mitosis and interphase. Thus, at the end of the 1960s the general picture that emerged from inspection of ultrathin sections of cell nuclei, isolated chromatin, and of whole mount preparations was that mitotic chromosomes and interphase chromatin consisted of a complex meshwork of irregularly sized and knobby fibers with diameters ranging from 20 to 30 nm (for reviews see Ris 1969; DuPraw 1970; Solari 1974; Ris and Korenberg 1979; Hozier 1979). Although the internal organization of chromatin fibers was particularly suitable for study by surface spreading on an air-water interface, originally introduced by Gall (1963), neither a discrete subunit organization was recognized nor could the “thick” chromatin fibers be reproducibly unfolded into thinner fibers which were believed to be transcriptionally active (Gall 1966). Hence, electron microscopic methods were unavailable to study features of chromosomes during interphase, i.e., their functional subdivision into transcriptionally active and inactive domains.
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References
Amabis JM, Nair KK (1976) Ultrastructure of gene transcription in spermatocytes of Trichosia pubescens Morgante, 1969 (diptera: sciaridae). Z Naturforsch 31c: 186–189
Angelier N, Lacroix JC (1975) Complexes de transcription d’origines nucléolaire et chromosomique d’ovocytes de Pleurodeles waltlii et P.poireti (amphibiens, urodèles). Chromosoma 51: 323–335
Angelier N, Hemon D, Bouteille M (1979) Mechanisms of transcription in nucleoli of amphibian oocytes as visualized by high-resolution autoradiography. J Cell Biol 80: 277–290
Angelier N, Hernandez-Verdun D, Bouteille M (1982) Visualization of Ag-NOR proteins on nucleolar transcriptional units in molecular spreads. Chromosoma 86: 661–672
Bachvarova R, Burns JP, Spiegelman I, Choy J, Chaganti RSK (1982) Morphology and transcriptional activity of mouse oocyte chromosomes. Chromosoma 86: 181–196
Bakken A, Morgan G, Sollner-Webb B, Roan J, Busby S, Reeder RH (1982) Mapping of transcription initiation and termination signal on Xenopus laevis ribosomal DNA. Proc Natl Acad Sci USA 79: 56–60
Beermann S (1984) Circular and linear structures in chromatin diminution of Cyclops. Chromo-soma 89: 321–328
Berger S, Schweiger H-G (1982) Characterization and species differences of rRNA in algae. In: Busch H, Rothblum L (eds) The cell nucleus, vol 10. Academic Press, New York, pp 31–65
Beyer AL, Miller OL, McKnight SL (1980) Ribonucleoprotein structure in nascent hnRNA is nonrandom and sequence-dependent. Cell 20: 75–84
Beyer AL, Bouton AH, Miller OL (1981) Correlation of hnRNP structure and nascent transcript cleavage. Cell 26: 155–165
Bona M, Scheer U, Bautz EKF (1981) Antibodies to RNA polymerase II ( B) inhibit transcription in lampbrush chromosomes after microinjection into living amphibian oocytes. J Mol Biol 151: 81–99
Borchsenius S, Bonven B, Leer JC, Westergaard O (1981) Nuclease-sensitive regions on the extrachromosomal r-chromatin from Tetrahymena pyriformis. Eur J Biochem 117: 245–250
Busby S, Bakken A (1979) A quantitative electron microscopic analysis of transcription in sea urchin embryos. Chromosoma 71: 249–262
Busby S, Bakken A (1980) Transcription in developing sea urchins: electron microscopic analysis of cleavage, gastrula and prism stages. Chromosoma 79: 85–104
Bustin M, Goldblatt D, Sperling R (1976) Chromatin structure visualization by immunoelectron microscopy. Cell 7: 297–304
Callan HG (1982) Lampbrush chromosomes. Proc R Soc Lond B 214: 417–448
Calvet JP, Pederson T (1979) Heterogeneous nuclear RNA double-stranded regions probed in living HeLa cells by crosslinking with the psoralen derivative aminomethyltrioxsalen. Proc Natl Acad Sci USA 76: 755–759
Chou WY (1976) RNA transcription and ribosomal protein assembly in Drosophila melano-gaster. In: King RC (ed) Handbook of genetics, vol 5. Plenum, New York, pp 219–265
Chooi WY, Leiby KR (1981) An electron microscopic method for localization of ribosomal pro-teins during transcription of ribosomal DNA: a method for studying protein assembly. Proc Natl Acad Sci USA 78: 4823–4827
Cotton RW, Manes C, Hamkalo BA (1980) Electron microscopic analysis of RNA transcription in preimplantation rabbit embryos. Chromosoma 79: 169–178
de Loos F, Dijkhof R, Grond CJ, Hennig W (1984) Lampbrush chromosome loop-specificity of transcript morphology in spermatocyte nuclei of Drosophila hydei. EM BO J 3: 28452849
Diaz MO, Gall JG (1985) Giant readthrough transcription units at the histone loci on lampbrush chromosomes of the newt Notophthalmus. Chromosoma 92: 243–253
Diaz MO, Barsacchi-Pilone G, Mahon KA, Gall JG (1981) Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus. Cell 24: 649–659
DuPraw EJ (1970) DNA and chromosomes. Holt, Rinehart and Winston, New York
Engberg J (1985) The ribosomal RNA genes of Tetrahymena: structure and function. Eur J Cell Biol 36: 133–151
Foe VE (1978) Modulation of ribosomal RNA synthesis in Oncopeltus fasciatus: an electron microscopic study of the relationship between changes in chromatin structure and transcriptional activity. Cold Spring Harbor Symp Quant Biol 42: 723–740
Foe VE, Wilkinson LE, Laird CD (1976) Comparative organization of active transcription units in Oncopeltus fasciatus. Cell 9: 131–146
Fostel J, Narayanswami S, Hamkalo B, Clarkson SG, Pardue ML (1984) Chromosomal location of a major tRNA gene cluster of Xenopus laevis. Chromosoma 90: 254–260
Franke WW, Scheer U (1978) Morphology of transcriptional units at different states of activity. Philos Trans R Soc Lond B 283: 333–342
Franke WW, Scheer U, Spring H, Trendelenburg MF, Krohne G (1976a) Morphology of transcriptional units of rDNA. Exp Cell Res 100: 233–244
Franke WW, Scheer U, Trendelenburg MF, Spring H, Zentgraf H (1976b) Absence of nucleosomes in transcriptionally active chromatin. Cytobiologie 13: 401–434
Franke WW, Scheer U, Trendelenburg MF, Zentgraf H, Spring H (1978) Morphology of tran-scriptionally active chromatin. Cold Spring Harbor Symp Quant Biol 42: 755–772
Franke WW, Scheer U, Spring H, Trendelenburg MF, Zentgraf H (1979) Organization of nu-cleolar chromatin. In: Busch H (ed) The cell nucleus, vol 7. Academic Press, New York, pp 49–95
Gall JG (1963) Chromosome fibers from an interphase nucleus. Science 139: 120–121
Gall JG (1966) Chromosome fibers studied by a spreading technique. Chromosoma 20: 221–233
Gall JG, Stephenson EC, Erba HP, Diaz MO, Barsacchi-Pilone G (1981) Histone genes are located at the sphere loci of newt lampbrush chromosomes. Chromosoma 84: 159–171
Ghose D, Weiss E, Homo JC, Van Regenmortel MHV, Oudet P (1983) Visualization of anti-histone antibodies on SV40 minichromosomes by scanning transmission electron microscopy ( STEM ). Exp Cell Res 147: 135–142
Glätzer KH (1975) Visualization of gene transcription in spermatocytes of Drosophila hydei. Chromosoma 53: 371–379
Glätzer KH (1980) Regular substructures within homologous transcripts of spread Drosophila germ cells. Exp Cell Res 125: 519–523
Glätzer KH, Meyer GF (1981) Morphological aspects of the genetic activity in primary spermatocyte nuclei of Drosophila hydei. Biol Cell 41: 165–172
Grainger RM, Maizels N (1980) Dictyostelium ribosomal RNA is processed during transcription. Cell 20: 619–623
Grainger RM, Ogle RC (1978) Chromatin structure of the ribosomal RNA genes in Physarum polycephalum. Chromosoma 65: 115–126
Greimers R, Deltour R (1981) Organization of transcribed and nontranscribed chromatin in isolated nuclei of Zea mays root cells. Eur J Cell Biol 23: 303–311
Grond CJ, Siegmund I, Hennig W (1983) Visualization of a lampbrush loop-forming fertility gene in Drosophila hydei. Chromosoma 88: 50–56
Hamkalo BA, Miller OL (1973) Electronmicroscopy of genetic activity. Annu Rev Biochem 42: 379–396
Hamkalo BA, Miller OL, Bakken AH (1973) Ultrastructure of active eukaryotic genomes. Cold Spring Harbor Symp Quant Biol 38: 915–919
Hamkalo BA, Goldsmith MR, Rattner JB (1981) Higher order structure in chromosomes. In: Schweiger HG (ed) International cell biology 1980–1981. Springer, Berlin Heidelberg New York, pp 152–161
Harper F, Puvion-Dutilleul F (1979) Non-nucleolar transcription complexes of rat liver as revealed by spreading isolated nuclei. J Cell Sci 40: 181–192
Harper F, Florentin Y, Puvion E (1984) Localization of T-antigen on simian virus 40 minichromosomes by immunoelectron microscopy. EMBO J 3: 1235–1241
Hennig W (1985) Y chromosome function and spermatogenesis in Drosophila hydei. Adv Genet 23: 179–234
Herzog M, Soyer M-O (1981) Distinctive features of dinoflagellate chromatin. Absence of nucleosomes in a primitive species Prorocentrum micans E. Eur J Cell Biol 23: 295–302
Hill RS (1979) A quantitative electron-microscope analysis of chromatin from Xenopus laevis lampbrush chromosomes. J Cell Sci 40: 145–169
Hill RS, MacGregor HC (1980) The development of lampbrush chromosome-type transcription in the early diplotene oocytes of Xenopus laevis: an electron-microscope analysis. J Cell Sci 44: 87–101
Howze GB, Hsie AW, Olins AL (1976) v-bodies in mitotic chromatin. Exp Cell Res 100:424–428
Hozier JC (1979) Nucleosomes and higher levels of chromosomal organization. In: Taylor JH (ed) Molecular genetics, part 3. Academic Press, New York, pp 315–385
Hügle B, Hazan R, Scheer U, Franke WW (1985) Localization of ribosomal protein 51 in the granular component of the interphase nucleolus and its distribution during mitosis. J Cell Biol 100: 873–886
Hughes ME, Bürki K, Fakan S (1979) Visualization of transcription in early mouse embryos. Chromosoma 73: 179–190
Igo-Kemenes T, Hörz W, Zachau HG (1982) Chromatin. Annu Rev Biochem 51: 89–121
Karpov VL, Preobrazhenskaya OV, Mirzabekov AD (1984) Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5’ region. Cell 36: 423–431
Kierszenbaum AL, Tres LL (1975) Structural and transcriptional features of the mouse spermatid genome. J Cell Biol 65: 258–270
Kornberg RD (1977) Structure of chromatin. Annu Rev Biochem 46: 931–954
Labhart P, Koller T (1982) Structure of the active nucleolar chromatin of Xenopus laevis oocytes. Cell 28: 279–292
Labhart P, Koller T, Wunderli H (1982) Involvement of higher order chromatin structures in metaphase chromosome organization. Cell 30: 115–121
Laird CD, Chooi WY (1976) Morphology of transcription units in Drosophila melanogaster. Chromosoma 58: 193–218
Laird CD, Wilkinson LE, Foe VE, Chooi WY (1976) Analysis of chromatin-associated fiber arrays. Chromosoma 58: 169–192
Lamb MM, Daneholt B (1979) Characterization of active transcription units in Balbiani rings of Chironomus tentans. Cell 17: 835–848
Landegent JE, Jansen in de Wal N, van Ommen GJB, Baas F, de Vijlder JJM, van Duijn P, van der Ploeg M (1985) Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization. Nature 317: 175–177
Lutz C, Nagl W (1980) A reliable method for preparation and electron microscopic visualization of nucleosomes in higher plant. Planta 149: 408–410
Mathis D, Oudet P, Chambon P (1980) Structure of transcribing chromatin. Prog Nucleic Acid Res Mol Biol 24: 1–55
McGhee JD, Felsenfeld G (1980) Nucleosome structure. Annu Rev Biochem 49: 1115–1156
McKnight SL, Miller OL (1976) Ultrastructural patterns of RNA synthesis during early embryo-genesis of Drosophila melanogaster. Cell 8: 305–319
McKnight SL, Sullivan NL, Miller OL (1976) Visualization of the silk fibroin transcription unit and nascent silk fibroin molecules on polyribosomes of Bombyx mori. Prog Nucleic Acid Res Mol Biol 19: 313–318
McKnight SL, Bustin M, Miller OL (1978) Electron microscopic analysis of chromosome metabolism in the Drosophila melanogaster embryo. Cold Spring Harbor Symp Quant Biol 42: 741–754
McKnight SL, Martin KA, Beyer AL, Miller OL (1979) Visualization of functionally active chromatin. In: Busch H (ed) The cell nucleus, vol 7. Academic Press, New York, pp 97–122
Meyer GF, Hennig W (1974) The nucleolus in primary spermatocytes of Drosophila hydei. Chromosoma 46: 121–144
Miller OL (1981) The nucleolus, chromosomes, and visualization of genetic activity. J Cell Biol 91: 15s - 27s
Miller OL (1984) Some ultrastructural aspects of genetic activity in eukaryotes. J Cell Sci Suppl 1: 81–93
Miller OL, Bakken AH (1972) Morphological studies of transcription. Acta Endocrinol Suppl 168: 155–177
Miller OL, Beatty BR (1969a) Visualization of nucleolar genes. Science 164: 955–957
Miller OL, Beatty BR (1969 b) Extrachromosomal nucleolar genes in amphibian oocytes. Genetics Suppl 61: 133–143
Miller OL, Beatty BR (1969e) Nucleolar structure and function. In: Lima-de-Faria A (ed) Handbook of molecular cytology. North-Holland, Amsterdam, pp 605–619
Miller OL, Beatty BR (1969d) Portrait of a gene. J Cell Physiol 74: Suppl 1: 225–232
Miller OL, Hamkalo BA (1972) Visualization of RNA synthesis on chromosomes. Int Rev Cytol 33: 1–25
Miller OL, Beatty BR, Hamkalo BA (1972) Nuclear structure and function during amphibian oogenesis. In: Biggers JD, Schuetz AW (eds) Oogenesis. Univ Park Press, Baltimore; Butter-worths, London, pp 119–129
Morgan GT, Reeder RH, Bakken AH (1983) Transcription in cloned spacers of Xenopus laevis ribosomal DNA. Proc Natl Acad Sci USA 80: 6490–6494
Mott MR, Callan HG (1975) An electron-microscope study of the lampbrush chromosomes of the newt Triturus cristatus. J Cell Sci 17: 241–261
N’Da E, Bonnanfant-Jais M L, Penrad-Mobayed M, Angelier N (1986) Size uniformity of ribonucleoprotein matrix particles in loops of Pleurodeles waltlü lampbrush chromosomes visualized by electron microscopy. J Cell Sci 81: 17–27
Oda T, Nakamura T, Watanabe S (1977) Transcription complexes of chromatin showing a beads-like structure of heterogeneous ribonucleoprotein chains. J Electron Microsc 26: 203–207
Old RW, Callan HG, Gross KW (1977) Localization of histone gene transcripts in newt lamp-brush chromosomes by in situ hybridization. J Cell Sci 27: 57–79
Olins AL, Olins DE (1973) Spheroid chromatin units (v bodies). J Cell Biol 59: 252a
Olins AL, Olins DE (1974) Spheroid chromatin units (v bodies). Science 183: 330–332
Olins AL, Carlson RD, Olins DE (1975) Visualization of chromatin substructure: v bodies. J Cell Biol 64: 528–537
Osheim YN, Beyer AL (1985) Nascent ribonucleoprotein structure of polymerase 1, II, and Ill gene transcripts. In: Smuckler EA, Clawson GA (cds) Nuclear envelope structure and RNA maturation. Liss, New York, pp 277–295
Osheim YN, Miller OL (1983) Novel amplification and transcriptional activity of chorion genes in Drosophila melanogaster follicle cells. Cell 33: 543–553
Osheim YN, Miller OL, Beyer AL (1985) RNP particles at splice junction sequences on Drosophila chorion transcripts. Cell 43: 143–151
Oudet P, Gross-Bellard M, Chambon P (1975) Electron microscopic and biochemical evidence that chromatin structure is a repeating unit. Cell 4: 281–300
Palen TE, Cech TR (1984) Chromatin structure at the replication origins and transcription-initiation regions of the ribosomal RNA genes of Tetrahymena. Cell 36: 933–942
Petrov P, Raitcheva E, Tsanev R (1980) Nucleosomes and nonribosomal RNA transcription in early mouse embryo. An electron microscopic study. Eur J Cell Biol 22: 708–713
Prior CP, Cantor CR, Johnson EM, Littau VC, Allfrey VG (1983) Reversible changes in nucleo-some structure and histone H3 accessibility in transcriptionally active and inactive states of rDNA chromatin. Cell 34: 1033–1042
Pruitt SC, Reeder RH (1984) Effect of topological constraint on transcription of ribosomal DNA in Xenopus oocytes. J Mol Biol 174: 121–139
Puvion-Dutilleul F (1983) Morphology of transcription at cellular and molecular levels. Int Rev Cytol 84: 57–101
Puvion-Dutilleul F, Bernadac A, Puvion E, Bernhard W (1977) Visualization of two different types of nuclear transcriptional complexes in rat liver cells. J Ultrastruct Res 58: 108–117
Rattner JB, Sauners C, Davie JR, Hamkalo BA (1982) Ultrastructural organization of yeast chromatin. J Cell Biol 92: 217–222
Reeder RH, Higashinakagawa T, Miller OL (1976) The 5’-3’ polarity of the Xenopus ribosomal RNA precursor molecule. Cell 8: 449–454
Reeder RH, McKnight SL, Miller OL (1978) Contraction ratio of the nontranscribed spacer of Xenopus rDNA chromatin. Cold Spring Harbor Symp Quant Biol 42: 1174–1177
Ris H (1969) The molecular organization of chromosomes. In: Lima-de-Faria A (ed) Handbook of molecular cytology. North-Holland, Amsterdam, pp 221–250
Ris H, Korenberg J (1979) Chromosome structure and levels of chromosome organization. In: Prescott DM, Goldstein L (eds) Cell biology, vol 2. Academic Press, New York, pp 267–361
Sargan DR, Butterworth PHW (1985) Eukaryotic ternary transcription complexes: transcription complexes of RNA polymerase II are associated with histone-containing, nucleosome-like particles in vivo. Nucl Acids Res 13: 3805–3822
Scheer U (1978) Changes of nucleosome frequency in nucleolar and non-nucleolar chromatin as a function of transcription: an electron microscopic study. Cell 13: 535–549
Scheer U (1980) Structural organization of spacer chromatin between transcribed ribosomal RNA genes in amphibian oocytes. Eur J Cell Biol 23: 189–196
Scheer U (1986) Injection of antibodies into the nucleus of amphibian oocytes: an experimental means of interfering with gene expression in the living cell. J Embryo] Exp Morph 97 Suppl: 223–242
Scheer U, Dabauvalle M-C (1985) Functional organization of the amphibian oocyte nucleus. In: Browder LW (ed) Developmental biology, vol 1. Plenum, New York, pp 385–430
Scheer U, Sommerville J (1982) Sizes of chromosome loops and hnRNA molecules in oocytes of amphibia of different genome sizes. Exp Cell Res 139: 410–416
Scheer U, Zentgraf H (1978) Nucleosomal and supranucleosomal organization of transcriptionally inactive rDNA circles in Dytiscus oocytes. Chromosoma 69: 243–254
Scheer U, Zentgraf H (1982) Morphology of nucleolar chromatin in electron microscopic spread preparations. In: Busch H, Rothblum L (eds) The cell nucleus, vol 11. Academic Press, New York, pp 143–176
Scheer U, Trendelenburg MF, Franke WW (1976a) Regulation of transcription of genes of ribosomal RNA during amphibian oogenesis. A biochemical and morphological study. J Cell Biol 69: 465–489
Scheer U, Franke WW, Trendelenburg MF, Spring H (1976b) Classification of loops of lamp-brush chromosomes according to the arrangement of transcriptional complexes. J Cell Sci 22: 503–519
Scheer U, Trendelenburg MF, Krohne G, Franke WW (1977) Lengths and patterns of transcriptional units in the amplified nucleoli of oocytes of Xenopus laevi.s. Chromosoma 60: 147–167
Scheer U, Spring H, Trendelenburg MF (1979a) Organization of transcriptionally active chromatin in lampbrush chromosome loops. In: Busch H (ed) The cell nucleus, vol 7. Academic Press, New York, pp 3–47
Scheer U, Sommerville J, Bustin M (1979 b) Injected histone antibodies interfere with transcription of lampbrush chromosome loops in oocytes of Pleurodele.s. J Cell Sci 40: 1–20
Scheer U, Zentgraf H, Sauer H (1981) Different chromatin structures in Physarum polycephalum. Chromosoma 84: 279–290
Seebeck T, Braun R (1982) Organization of rDNA in chromatin: Physarum. In: Busch H, Roth-blum L (eds) The cell nucleus, vol 11. Academic Press, New York, pp 177–191
Skoglund U, Andersson K, Björkroth B, Lamb MM, Daneholt B (1983) Visualization of the for-mation and transport of a specific hnRNP particle. Cell 34: 847–855
Solari AJ (1974) The molecular organization of the chromatin fiber. In: Busch H (ed) The cell nucleus, vol 1. Academic Press, New York, pp 493–535
Sollner-Webb B, Wilkinson JK, Miller KG (1982) Transcription of Xenopus ribosomal RNA genes. In: Busch H, Rothblum L (eds) The cell nucleus, vol 12. Academic Press, New York, pp 31–67
Sommerville J (1981) Immunolocalization and structural organization of nascent RNP. In: Busch H (ed) The cell nucleus, vol 8. Academic Press, New York, pp 1–57
Spring H, Franke WW (1981) Transcriptionally active chromatin in loops of lampbrush chromosomes at physiological salt concentration as revealed by electron microscopy sections. Eur J Cell Biol 24: 298–308
Spring H, Scheer U, Franke WW, Trendelenburg MF (1975) Lampbrush-type chromosomes in the primary nucleus of the green alga Acetabularia mediterranea. Chromosoma 50: 25–43
Spring H, Krohne G, Franke WW, Scheer U, Trendelenburg MF (1976) Homogeneity and heterogeneity of sizes of transcriptional units and spacer regions in nucleolar genes of Acetabularia. Biol Cell 25: 107–116
Trendelenburg MF (1981) Initiations of transcription at distinct promoter sites in spacer regions between pre-rRNA genes in oocytes of Xenopus laevis: an electron microscopic analysis. Biol Cell 42: 1–12
Trendelenburg MF (1983) Progress in visualization of eukaryotic gene transcription. Hum Genet 63: 197–215
Trendelenburg MF, McKinnell RG (1979) Transcriptionally active and inactive regions of nucleolar chromatin in amplified nucleoli of fully grown oocytes of hibernating frogs, Rana pipiens (amphibia, anura). Differentiation 15: 73–95
Trendelenburg MF, Scheer U, Zentgraf H, Franke WW (1976) Heterogeneity of spacer lengths in circles of amplified ribosomal DNA of two insect species, Dytiscus marginalis and Acheta domesticus. J Mol Biol 108: 453–470
Vavra KJ, Colavito-Shepanski M, Gorovsky MA (1982) Organization of rDNA in chromatin: Tetrahymena. In: Busch H, Rothblum L (eds) The cell nucleus, vol 11. Academic Press, New York, pp 193–223
Villard D, Fakan S (1978) Visualisation des complexes de transcription dans la chromatine étalée de mammifères: étude en autoradiographie à haute résolution. C R Acad Sci Paris 286D: 777–780
Weiss E, Chose D, Schultz P, Oudet P (1985) T-antigen is the only detectable protein on the nucleosome-free origin region of isolated simian virus 40 minichromosomes. Chromosoma 92: 391–400
Widmer RM, Lucchini R, Lezzi M, Meyer B, Sogo JM, Edström J-E, Koller T (1984) Chromatin structure of a hyperactive secretory protein gene (in Balbiani ring 2) of Chironomus. EMBO J 3: 1635–1641
Woodcock CLF (1973) Ultrastructure of inactive chromatin. J Cell Biol 59: 368a
Woodcock CLF, Safer JP, Stanchfield JE (1976) Structural repeating units in chromatin. Exp Cell Res 97: 101–110
Zentgraf H, Müller U, Franke WW (1980 a) Reversible in vitro packing of nucleosomal filaments into globular supranucleosomal units in chromatin of whole chick erythrocyte nuclei. Eur J Cell Biol 23: 171–188
Zentgraf H, Müller U, Franke WW (1980 b) Supranucleosomal organization of sea urchin sperm chromatin in regularly arranged 40 to 50 nm large granular subunits. Eur J Cell Biol 20:254–264
Zentgraf H, Müller U, Scheer U, Franke WW (1981) Evidence for the existence of globular units in the supranucleosomal organization of chromatin. In: Schweiger HG (ed) International cell biology 1980–1981. Springer, Berlin Heidelberg New York, pp 139–151
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Scheer, U. (1987). Contributions of Electron Microscopic Spreading Preparations (“Miller Spreads”) to the Analysis of Chromosome Structure. In: Hennig, W. (eds) Structure and Function of Eukaryotic Chromosomes. Results and Problems in Cell Differentiation, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-47783-9_10
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