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Heat Shock and Development pp 188–209Cite as

Transforming Growth Factor-ß Regulates Basal Expression of the hsp70 Gene Family in Cultured Chicken Embryo Cells

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Part of the book series: Results and Problems in Cell Differentiation ((RESULTS,volume 17))

Abstract

During studies of stress protein induction by heavy metal ions (Whelan and Hightower 1985), it was noticed that the levels of newly synthesized heat shock proteins (Hsps) especially Hsp90 along with Hsp70 and-or its constitutive form, Hsc70, were reduced in chicken embryo cell (CEC) cultures incubated without serum relative to serum-containing control cultures. We suspected that growth factors in calf serum might be responsible for this effect. However, after a false start involving an early commercial preparation of platelet-derived growth factor (PDGF) that initially produced an effect on Hsp90 and Hsp70 accumulation that proved to be due to a contaminant, we set the observation aside. A visit to M. Sporn’s laboratory in 1987 rekindled our interest in growth factors as potential regulators of heat shock gene expression. In particular, Sporn suggested to us that transforming growth factor-ß (TGF-ß), which only recently has been implicated in wound responses, might play such a role (Sporn et al. 1987). Shortly thereafter, I. Takenaka began her Ph.D. thesis research in the Hightower laboratory, and our renewed interest coupled with her prior experience with growth factors led us to carry out a systematic search among growth factors derived from platelets for any capable of stimulating stress protein accumulation in either CECs or an array of mammalian cell lines. TGF-ß and CECs proved to be the winning combination in our survey. The rates of accumulation of Hsp90 and three members of the Hsp70 family including Hsp70, Hsc70, and the 78 kDa glucose-regulated protein, Grp78, were all stimulated by TGF-ß in serum-free culture medium. A detailed analysis of the level of regulation of basal expression of the genes encoding these proteins was carried out, and a preliminary report on these data for the hsp70 gene family is presented here.

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  1. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut, 06269-3044, USA

    Lawrence E. Hightower

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  1. Ivone M. Takenaka
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  2. Seth Sadis
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  3. Lawrence E. Hightower
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  1. Department of Molecular and Cell Biology, The University of Connecticut, 75 North Eagleville Road, 06269-3044, Storrs, CT, USA

    Lawrence Hightower

  2. Institut für Biochemie der Pflanzen, Weinberg 3, D-4050, Halle (O), Federal Republic of Germany

    Lutz Nover

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Takenaka, I.M., Sadis, S., Hightower, L.E. (1991). Transforming Growth Factor-ß Regulates Basal Expression of the hsp70 Gene Family in Cultured Chicken Embryo Cells. In: Hightower, L., Nover, L. (eds) Heat Shock and Development. Results and Problems in Cell Differentiation, vol 17. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-46712-0_13

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