Skip to main content
SpringerLink
Log in
Book cover

Applications of Synchrotron Radiation pp 47–105Cite as

SR Microbeam Analysis at Cellular Level

  • Chapter

  • 866 Accesses

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

Abstract

The advent of microbeam analytical methods has opened up new possibilities of studies of biological systems at the cellular level. In situ techniques enable analysis of living cells in vitro and even in vivo. Electron microprobes and proton microprobes can be used for such studies, but thermal damage to the sample precludes their extensive use. Synchrotron radiation-based microbeams provide virtually an ideal tool for this area of study, offering sensitivity for detection of elements at the parts-per-million level comparable to proton microprobes, but without the adverse effect of sample damage. Although the information is limited to only elemental composition and not the actual compounds, SR also offers the additional advantage of enabling chemical state analysis at trace levels. In this chapter a number of applications of SR microbeam analysis at the cellular level will be presented to illustrate the power of the method.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. A.M. Ektessabi, M. Rokkum, C. Johansson, T. Albrektsson, L. Sennerby, H. Saisho, S. Honda, J. Synchrotron Rad., 1998, 5, 1136.

    Article  Google Scholar 

  2. A.M. Ektessabi, J. Mouhyi, P. Louvette, L. Sennerby, International Journal of PIXE, 1997, 7, 179.

    Article  Google Scholar 

  3. A.M. Ektessabi, A. Wennerberg, International Journal of PIXE, 1995, 5, 145.

    Article  Google Scholar 

  4. J.C. Wang, W.D. Yu, H.S. Sandhu, F. Betts, S. Bhuta, R.B. Delamarter, Spine, 1999, 24, 899.

    Article  Google Scholar 

  5. J.Y. Wang, B.H. Wicklund, R.B. Gustilo, D.T. Tsukayama, Biomaterials, 1996, 17, 2233.

    Article  Google Scholar 

  6. A.S. Shanbhag, J.J. Jacobs, J. Black, J.O. Galante, T.T. Glant, J. Biomed. Mater. Res., 1994, 28, 81.

    Article  Google Scholar 

  7. W.J. Kao, Biomaterials, 1999, 20, 2213.

    Article  Google Scholar 

  8. R. Geertz, H. Gulyas, G. Gercken, Toxicology, 1994, 86, 13.

    Article  Google Scholar 

  9. T. Pinheiro, M.L. Carvalho, C. Casaca, M.A. Barreiros, A.S. Cubha, P. Chevallier, Nucl. Instr. and Meth. B, 1999, 158, 393.

    Article  ADS  Google Scholar 

  10. P. Goegan, R. Vincent, P. Kumarathasan, J. Brook, Toxicol. in vitro, 1998, 12, 25.

    Article  Google Scholar 

  11. M. Radloff, M. Delling, G. Gercken, Toxicol. Lett., 1998, 96, 69.

    Article  Google Scholar 

  12. G. Gercken, I. Berg, M. Dorger, T. Schluter, Toxicol. Lett., 1996, 88, 121.

    Article  Google Scholar 

  13. J.C. Wataha, C.T. Hanks, Z. Sun, Dental Materials, 1995, 11, 239.

    Article  Google Scholar 

  14. S. Meresse, O. Steele-Mortimer, E. Moreno, M. Desjardins, B. Finlay, J.P. Gorvel, Nature Cell Biology, 1999, 1, E183.

    Article  Google Scholar 

  15. D. Granchi, G. Ciapetti, S. Stea, L. Savarino, F. Filippini, A. Sudanese, G. Zinghi, L. Montanaro, Biomaterials, 1999, 20, 1079.

    Article  Google Scholar 

  16. A. Aderem, D.M. Underhill, Annu. Rev. Immunol., 1999, 17, 593.

    Article  Google Scholar 

  17. D.M. Underhill, A. Ozinsky, A.M. Hajjar, A. Stevens, C.B. Wilson, M. Bassetti, A. Aderem, Nature, 1999, 401, 811.

    Article  ADS  Google Scholar 

  18. J.A. Hunt, P.J. McLaughlin, B.F. Flanagan, Biomaterials, 1997, 18, 1449.

    Article  Google Scholar 

  19. A.M. Ektessabi, S. Yoshida, K. Takada, X-ray Spectrometry, 1999, 28, 456.

    Article  Google Scholar 

  20. F. Supek, L. Supekova, H. Nelson, N. Nelson, J. Exp. Biol., 1997, 200, 321.

    Google Scholar 

  21. C.E. Lewis, J. McGee, “The Macrophage”, IRL press, 1992.

    Google Scholar 

  22. C.W. Olanow, G.W. Arendash, Curr. Opin. Neurol., 1994, 7, 548.

    Article  Google Scholar 

  23. S.L. Sensi, H.Z. Yin, S.G. Carriedo, S.S. Rao, J.H. Weiss, Neurobiology, 1999, 96, 2414.

    Google Scholar 

  24. K.M. Noh, Y.H. Kim, J.Y. Koh, J. Neurochem., 1999, 72, 1609.

    Article  Google Scholar 

  25. S. Orrenius, M.J. Burkitt, G.E. Kass, J.M. Dypbukt, P. Nicotera, Ann. Neurol., 1992, 32, S33.

    Article  Google Scholar 

  26. E. Kienzl, L. Puchinger, K. Jellinger, W. Linert, H. Stachelberger, R.F. Jameson, J. Neurol. Sci., 1995, 134, S69.

    Article  Google Scholar 

  27. K. Abreo, F. Abreo, M.L. Sella, S. Jain, J. Neurochem., 1999, 72, 2059.

    Article  Google Scholar 

  28. K. Zaman, H. Ryu, D. Hall, K. O’Donovan, K. Lin, M.P. Miller, J.C. Marquis, J.M. Baraban, G.L. Semenza, R.R. Ratan, J. Neurosci., 1999, 15, 9821.

    Google Scholar 

  29. W.Y. Ong, M.Q. Ren, J. Makjanic, T.M. Lim, F. Watt, J. Neurochem., 1999, 72, 1574.

    Article  Google Scholar 

  30. P.F. Good, D.P. Perl, L.M. Bierer, J. Schmeidler, Ann. Neurol., 1992, 31, 286.

    Article  Google Scholar 

  31. A.R. White, A.I. Bush, K. Beyreuther, C.L. Masters, R. Cappai, J. Neurochem., 1999, 72, 2092.

    Article  Google Scholar 

  32. P.F. Good, C.W. Olanow, D.P. Perl, Brain Res., 1992, 593, 343.

    Article  Google Scholar 

  33. K. Takada, A.M. Ektessabi, S. Yoshida, AIP Conference Proceedings, 1999, 475, 452.

    Article  ADS  Google Scholar 

  34. S. Yoshida, K. Takada, A.M. Ektessabi, AIP Conference Proceedings, 1999, 475, 611.

    Article  ADS  Google Scholar 

  35. P.D. Griffiths, B.R. Dobson, G.R. Jones, D.T. Clarke, Brain, 1999, 122, 667.

    Article  Google Scholar 

  36. K.D. Barron, J. Neurol. Sci., 1995, 134, 57.

    Article  Google Scholar 

  37. H. Lodish, D. Baltimore, A. Berk, “Molecular Cell Biology”, third edition, W.H. Freeman & Co, 1995.

    Google Scholar 

  38. J.R. Burdo, J. Martin, S.L. Menzies, K.G. Dolan, M.A. Romano, R.J. Fletcher, M.D. Garrick, L.M. Garrick, J.R. Connor, Neuroscience, 1999, 93, 1189.

    Article  Google Scholar 

  39. P. Ponka, Blood, 1997, 89, 1.

    Google Scholar 

  40. K.L. Double, M. Maywald, M. Schmittel, P. Riederer, M. Gerlach, J. Neurochem., 1998, 70, 2492.

    Article  Google Scholar 

  41. S. Chen, K.K. Sulik, Pharmacol., 2000, 294, 134.

    Google Scholar 

  42. G. Weiss, G. Werner-Felmayer, E.R. Werner, K. Grunewald, H. Wachter, M.W. Hentze, J. Exp. Med., 1994, 180, 969.

    Article  Google Scholar 

Download references

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

(2007). SR Microbeam Analysis at Cellular Level. In: Applications of Synchrotron Radiation. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-46427-3_4

Download citation

Publish with us

Policies and ethics

Search

Navigation