Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

  • Matthew R. Arnison
  • Carol J. Cogswell
  • Colin J. R. Sheppard
  • Peter Török
Part of the Springer Series in Optical Sciences book series (SSOS, volume 87)


In recent years live cell fluorescence microscopy has become increasingly important in biological and medical studies. This is largely due to new genetic engineering techniques which allow cell features to grow their own fluorescent markers. A popular example is green fluorescent protein. This avoids the need to stain, and thereby kill, a cell specimen before taking fluorescence images, and thus provides a major new method for observing live cell dynamics.


Spatial Frequency Point Spread Function Numerical Aperture Phase Mask Unwrap Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    C. J. R. Sheppard: J. Microsc. 149, 73 (1988)CrossRefGoogle Scholar
  2. 2.
    B. Richards, E. Wolf: Proc. Roy. Soc. A 253, 358 (1959)ADSzbMATHCrossRefGoogle Scholar
  3. 3.
    G. Häusler: Opt. Commun. 6, 38 (1972)ADSCrossRefGoogle Scholar
  4. 4.
    R. Juˇskaitis, M. A. A. Neil, F. Massoumian, T. Wilson: ‘Strategies for wide-field extended focus microscopy’. In: Focus on microscopy conference (Amsterdam, 2001 )Google Scholar
  5. 5.
    C. J. R. Sheppard, D. K. Hamilton, I. J. Cox: Proc. R. Soc. Lond. A A387, 171 (1983)ADSCrossRefGoogle Scholar
  6. 6.
    P. Potuluri, M. Fetterman, D. Brady: Opt. Express 8, 624 (2001)ADSCrossRefGoogle Scholar
  7. 7.
    J. Ojeda-Casta˜neda, R. Ramos, A. Noyola-Isgleas: Appl. Opt. 27, 2583 (1988)Google Scholar
  8. 8.
    J. Ojeda-Casta˜neda, E. Tepichin, A. Diaz: Appl. Opt. 28, 2666 (1989)Google Scholar
  9. 9.
    W. T. Welford: J. Opt. Soc. Am. 50, 749 (1960)ADSCrossRefGoogle Scholar
  10. 10.
    J. Campos, J. C. Escalera, C. J. R. Sheppard, M. J. Yzuel: J. Mod. Optics 47, 57 (2000)ADSGoogle Scholar
  11. 11.
    T. Wilson, M. A. A. Neil, F. Massoumian: ‘Point spread functions with extended depth of focus’. In: Proc. SPIE, volume 4621 ( San Jose, CA, 2002 ) pp. 28–31Google Scholar
  12. 12.
    S. Bradburn, W. T. Cathey, E. R. Dowski, Jr.: App. Opt. 36, 9157 (1997)ADSCrossRefGoogle Scholar
  13. 13.
    E. R. Dowski, Jr., W. T. Cathey: App. Opt. 34, 1859 (1995)ADSGoogle Scholar
  14. 14.
    W. Chi, N. George: Opt. Lett. 26, 875 (2001)ADSCrossRefGoogle Scholar
  15. 15.
    S. C. Tucker, W. T. Cathey, E. R. Dowski, Jr.: Optics Express 4, 467 (1999)ADSCrossRefGoogle Scholar
  16. 16.
    P. D. Higdon, P. Török, T. Wilson: J. Microsc. 193, 127 (1999)CrossRefGoogle Scholar
  17. 17.
    P. Török, P. Varga, Z. Laczik, G. R. Booker: J. Opt. Soc. Am. A 12, (325) (1995)Google Scholar
  18. 18.
    B. R. Frieden: J. Opt. Soc. Am. 57, 56 (1967)ADSCrossRefGoogle Scholar
  19. 19.
    C. W. McCutchen: J. Opt. Soc. Am. 54, 240 (1964)ADSCrossRefGoogle Scholar
  20. 20.
    C. J. R. Sheppard, M. Gu, Y. Kawata, S. Kawata: J. Opt. Soc. Am. A 11, 593 (1994)ADSCrossRefGoogle Scholar
  21. 21.
    M. R. Arnison, C. J. R. Sheppard: Opt. Commun. 211, 53 (2002)ADSCrossRefGoogle Scholar
  22. 22.
    C. J. R. Sheppard, K. G. Larkin: Optik 107 79 (1997)Google Scholar
  23. 23.
    M. Mansuripur: J. Opt. Soc. Am. A 3, 2086 (1986)ADSCrossRefGoogle Scholar
  24. 24.
    H. H. Hopkins: Proc. Phys. Soc. 55, 116 (1943)ADSCrossRefGoogle Scholar
  25. 25.
    C. J. R. Sheppard, C. J. Cogswell: ‘Three-dimensional imaging in confocal microscopy’. In: Confocal microscopy ( Academic Press, London 1990 ) pp. 143–169.Google Scholar
  26. 26.
    R. N. Bracewell: Two-dimensional imaging ( Prentice Hall, Englewood Cliffs, NJ 1995 )zbMATHGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Matthew R. Arnison
  • Carol J. Cogswell
  • Colin J. R. Sheppard
  • Peter Török

There are no affiliations available

Personalised recommendations