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Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

  • Matthew R. Arnison
  • Carol J. Cogswell
  • Colin J. R. Sheppard
  • Peter Török
Part of the Springer Series in Optical Sciences book series (SSOS, volume 87)

Abstract

In recent years live cell fluorescence microscopy has become increasingly important in biological and medical studies. This is largely due to new genetic engineering techniques which allow cell features to grow their own fluorescent markers. A popular example is green fluorescent protein. This avoids the need to stain, and thereby kill, a cell specimen before taking fluorescence images, and thus provides a major new method for observing live cell dynamics.

Keywords

Spatial Frequency Point Spread Function Numerical Aperture Phase Mask Unwrap Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Matthew R. Arnison
  • Carol J. Cogswell
  • Colin J. R. Sheppard
  • Peter Török

There are no affiliations available

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