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Exploring Living Cells and Molecular Dynamics with Polarized Light Microscopy

  • S. Inoué
Part of the Springer Series in Optical Sciences book series (SSOS, volume 87)

Abstract

Here, I would like to discuss the reasons, and methods, for using polarized light microscopy for studying the organization, and function, of living cells. In illustrating these points, I will be relying on some biological examples that I am particularly familiar with, namely, those related to the events of mitosis and cell division, which represent some of the more fundamental and critical events in the life of all living organisms.

Keywords

Polarize Light Microscopy Meiotic Spindle Liquid Crys Extinction Factor Cell Plate Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    H. Ambronn, A Frey: Das Polarisationsmikroskop, seine Anwedung in der Kolloidforschung in der Farbarei ( Akademische Verlag, Leipzig 1926 )Google Scholar
  2. 2.
    W.J. Schmidt: ‘Die Doppelbrechung von Zytoplasma, Karyoplasma und Metaplasma’, In: Protoplasma Monographien, Vol. 11 ( Borntraeger, Berlin 1937 )Google Scholar
  3. 3.
    M.M. Swann, M. Mitchison: J. Exp. Biol. 27, 226 (1950)Google Scholar
  4. 4.
    S. Inoué, K. Dan: J. Morphol. 89, 423 (1951)CrossRefGoogle Scholar
  5. 5.
    S. Inoué, W.L. Hyde: J. Biophys., and Biochem. Cytol. 3, 831 (1957)CrossRefGoogle Scholar
  6. 6.
    S. Inoué, H. Kubota: Nature 182, 1725 (1958)ADSCrossRefGoogle Scholar
  7. 7.
    H. Kubota, S. Inoué: J. Opt. Soc. Am. 49, 191 (1959)ADSCrossRefGoogle Scholar
  8. 8.
    S. Inoué, H. Sato: ‘Deoxyribonucleic Acid Arrangement in Living Sperm’, In: Molecular Architecture in Cell Physiology, ed. by T. Hayashi, A. Szent-Györgyi ( Prentice Hall, New Jersey 1966 ) pp. 209–248Google Scholar
  9. 9.
    M. Shribak, S. Inoué, R. Oldenbourg: Opt. Eng. 43, 943 (2002)ADSCrossRefGoogle Scholar
  10. 10.
    S. Inoué, K.R. Spring: Video Microscopy –The Fundamentals ( Plenum Press, New York 1997 )CrossRefGoogle Scholar
  11. 11.
    G.W. Ellis: J. Cell Biol. 101, 83a (1985)CrossRefGoogle Scholar
  12. 12.
    S. Inoué, R. Oldenbourg: Mol. Biol. Cell. 9, 1603 (1998)Google Scholar
  13. 13.
    A. Bajer, J. Mol`e–Bajer: Chromosoma 7, 558 (1956)CrossRefGoogle Scholar
  14. 14.
    A. Bajer, J. Mol`e–Bajer: J. Cell Biol. 102, 263 (1986)CrossRefGoogle Scholar
  15. 15.
    S. Inoué: Studies of the Structure of the Mitotic Spindle in Living Cells with an Improved Polarization Microscope PhD Thesis (Princeton University, New Jersey 195 1 )Google Scholar
  16. 16.
    S. Inoué: J. Cell Biol. 91, 131s (1981b)CrossRefGoogle Scholar
  17. 17.
    S. Inoué, H. Sato: J. Gen. Physiol. 50, 259 (1967)CrossRefGoogle Scholar
  18. 18.
    S. Inoué, E.D. Salmon: Mol. Biol. Cell. 6, 1619 (1995)Google Scholar
  19. 19.
    S. Inoué: ‘Organization and Function of the Mitotic Spindle’, In: Primitive Motile Systems in Cell Biology, ed. by R.D. Allen, N. Kamiya ( Academic Press, New York 1964 ) pp. 549–598CrossRefGoogle Scholar
  20. 20.
    R. Weisenberg: Science 177, 1196 (1972)CrossRefGoogle Scholar
  21. 21.
    J.B. Olmsted, G.G. Borisy: Biochemistry 14, 2996 (1975)CrossRefGoogle Scholar
  22. 22.
    L.G. Tilney, S. Inoué: J. Cell Biol. 93, 820 (1982)CrossRefGoogle Scholar
  23. 23.
    R.D. Allen, J.L. Travis, N.S. Allen, H. Yilmas: Cell Motil. 1, 275 (1981)CrossRefGoogle Scholar
  24. 24.
    S. Inoué: J. Cell Biol. 89, 346 (1981)CrossRefGoogle Scholar
  25. 25.
    S. Inoué, T.D. Inoué: Biol. Bull. 187, 232 (1994)Google Scholar
  26. 26.
    R. Oldenbourg: Nature 381, 811 (1996)ADSCrossRefGoogle Scholar
  27. 27.
    R. Oldenbourg, E.D. Salmon, P.T. Tran: Biophys. J. 74, 645 (1998)CrossRefGoogle Scholar
  28. 28.
    S. Inoué: FASEB J. 13, S185 (1999)Google Scholar
  29. 29.
    S. Inoué, R.A. Knudson, M. Goda, K. Suzuki, C. Nagano, N. Okada, H. Takahashi, K. Ichie, M. Iida,K. Yamanaka: J. Microsc. –- Oxford 201, 341 (2001)CrossRefGoogle Scholar
  30. 30.
    S. Inoué, M. Goda, R.A. Knudson: J. Microsc. –Oxford 201, 357 (2001)CrossRefGoogle Scholar
  31. 31.
    S. Inoué, M. Goda: Biol. Bull. 201, 231 (2001)CrossRefGoogle Scholar
  32. 32.
    S. Inoué, O. Shimomura, M. Goda, M. Shribak, P.T. Tran: P. Natl. Acad. Sci. USA 99, 4272 (2002)Google Scholar
  33. 33.
    K. Kinosita Jr.: FASEB J. 13, S201 (1999)Google Scholar

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© Springer-Verlag Berlin Heidelberg 2003

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  • S. Inoué

There are no affiliations available

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