Abstract
Three procedures are in general use for the formation and detection of RNA-DNA hybrids: reactions on a filter (Gillespie and Spiegelman, 1965); reaction in liquid (Nygaard and Hall, 1963); and reaction in agar (McCarthy and Bolton, 1964). The inherent limitations of each procedure have been studied in detail (Kennell and Kotoulas, 1968). Because of its simplicity, the filter method has been used in various phases of nucleic acid research with good reliability and convenience. Thus it has become possible to estimate the size of that portion of the genome specific for a species of RNA and to measure the appearance of a particular species of RNA during development. However, because of the possibility of nonspecific binding of added RNA to some material, such as basic proteins (which might also render such spuriously bound RNA resistant to RNase), it has been assumed that purity of the DNA fixed on the nitrocellulose filter is imperative.
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© 1972 Springer-Verlag Berlin Heidelberg
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Kim, KH. (1972). Nucleic Acid Hybridization to Isolated Chromatin. In: Ursprung, H. (eds) Nucleic Acid Hybridization in the Study of Cell Differentiation. Results and Problems in Cell Differentiation, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-37149-6_4
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DOI: https://doi.org/10.1007/978-3-540-37149-6_4
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