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A calorimetric biosensor and its application for detecting a cancer cell with optical imaging

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Part of the IFMBE Proceedings book series (IFMBE,volume 14)

Abstract

This paper reports a biochemical sensor for a label-free detection of cancer cell. A microcalorimeter has monitored the signal created from generated heat due to biochemical reaction between cancer cell and antibody in real time. HER2/neu receptor which is a remarkable biomarker of several cancers, especially breast cancer, was detected by using antibody, in this case HERCEPTIN®. In this study, the microcalorimeter was fabricated by using MEMS (Micro Electro Mechanical Systems)-technology. For higher sensitivity of microcalorimeter, it is important to reduce a parasitic heat transfer from a thermopile to a silicon substrate which has higher thermal conductivity (k = 148 W/m·K). Hot junctions of the proposed microcalorimeter are released from a silicon substrate by bulk micromachining to reduce loss of generated heat by reactions between biomolecules. NIH3T6.7 cell line with overexpressed HER2/neu receptor and FITC conjugated HERCEPTIN® were used. Each volume of biomolecules was 10 µl. HER2/neu receptor was detected by measuring output voltage change of the microcalorimeter about 7.92V/mole. For confirming a reliability of data, the measured data was compared with fluorescent data.

Keywords

  • Microcalorimeter
  • bulk micromachining
  • HER2/neu receptor
  • HERCEPTIN
  • optical imaging

College of medicine, Department of radiology, Yonsei University, Seoul, Korea 120-749

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Correspondence to Se-Chul Park .

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© 2007 International Federation for Medical and Biological Engineering

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Park, SC. et al. (2007). A calorimetric biosensor and its application for detecting a cancer cell with optical imaging. In: Magjarevic, R., Nagel, J.H. (eds) World Congress on Medical Physics and Biomedical Engineering 2006. IFMBE Proceedings, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-36841-0_147

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  • DOI: https://doi.org/10.1007/978-3-540-36841-0_147

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-36839-7

  • Online ISBN: 978-3-540-36841-0

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