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Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders

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Conditional Mutagenesis: An Approach to Disease Models

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 178))

Abstract

The advent of technologies that allow conditional mutagenesis has revolutionized our ability to explore gene functions and to establish animal models of human diseases. Both aspects have proven to be of particular importance in the study of lipid-related disorders. Classical approaches to gene inactivation by conventional gene targeting strategies have been successfully applied to generate animal models like the LDL receptor- and the apolipoprotein E-knockout mice, which are still widely used to study diverse aspects of atherosclerosis, lipid transport, and neurodegenerative disease. In many cases, however, simply inactivating the gene of interest has resulted in early lethal or complex phenotypes which are difficult to interpret. In recent years, additional tools have therefore been developed that allow the spatiotemporally controlled manipulation of the genome, as described in detail in Part I of this volume. Our aim is to provide an exemplary survey of the application of different conditional mutagenesis techniques in lipid research in order to illustrate their potential to unravel physiological functions of a broad range of genes involved in lipid homeostasis.

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Authors and Affiliations

  1. Zentrum für Neurowissenschaften, Universität Freiburg, Albertstrasse 23, 79104, Freiburg, Germany

    H. H. Bock & P. May

  2. Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, 75390-9046, USA

    J. Herz

  3. Innere Medizin II, Universitätsklinikum Freiburg, 79106, Freiburg, Germany

    H. H. Bock & P. May

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  1. H. H. Bock
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  1. Interfakultäres Institut für Biochemie (IFIB) Signaltransduktion — transgene Modelle, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Robert Feil

  2. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1, rue Laurent Fries, 67404, Illkirch, France

    Daniel Metzger

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Bock, H.H., Herz, J., May, P. (2007). Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders. In: Feil, R., Metzger, D. (eds) Conditional Mutagenesis: An Approach to Disease Models. Handbook of Experimental Pharmacology, vol 178. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35109-2_17

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