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Technetium-99m Pharmaceuticals pp 103–122Cite as

Cellular Labeling with 99mTc Chelates: Relevance of In Vitro and In Vivo Viability Testing

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8.6 Conclusions

Developments in both instrumentation and cell labeling have enabled a great expansion of their use in both clinical practice and research.

Studies with 99mTc-RBC are in widespread use in clinical practice. In contrast, 99mTc has not been successfully applied to platelet labeling. In contrast to 99mTc-RBC-research, there have been more methodological reports with 99mTc-WBC than clinical applications at the present. Much progress has been achieved in the techniques of harvesting and labeling WBC, but many challenges remain. HMPAO is currently the best 99mTc agent for labeling WBC, and played a particularly important role for the successful expansion of diagnostic imaging with radiolabeled WBC in nuclear medicine.

New approaches, such as the use of peptides, proteins, antibodies, and molecular recognition unit technologies, may result in substantial improvements in the labeling methodology and could yield labeled cells with the least damage and high in vivo stability in the future.

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  1. Department of Nuclear Medicine, Medical University Vienna, 1090, Vienna, Austria

    H. Sinzinger & M. Rodrigues

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  1. Department of Medicinal/Pharmaceutical Chemistry, University of Vienna, Althanstraße 14, 1090, Vienna, Austria

    Ilse Zolle

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Sinzinger, H., Rodrigues, M. (2007). Cellular Labeling with 99mTc Chelates: Relevance of In Vitro and In Vivo Viability Testing. In: Zolle, I. (eds) Technetium-99m Pharmaceuticals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-33990-8_8

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