Abstract
Immunochemical analysis for environmental monitoring is so far predominantly based on antibodies derived from sera and hybridoma cultures. This conventional technology will be supported or even replaced in the near future by the recombinant antibody (Rab) approach, since Rab are accessible to precise modification of antibody properties by virtue of genetic engineering. Furthermore, the in vitro generation of Rab libraries bearing an antibody diversity comparable to the vertebrate immune repertoire paves the way to substitute animals as the antibody source of choice. Utilising the s-triazine herbicides as target molecules, various strategies for the synthesis of Rab libraries are presented comprising natural, semisynthetic and synthetic antibody genes. The Rab genes are expressed as fusion proteins at the surface of phage particles. Special attention is given to the establishment of an efficient selection procedure. Phages presenting specific Rab are isolated via immuno affinity chromatography employing s-triazine coated beads. The described libraries are designed as a gene pool to provide an array of antibody variants for future immunosensor applications in environmental analysis.
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© 1998 B. G. Teubner Verlagsgesellschaft Leipzig
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Kramer, K., Knappik, A. (1998). Strategies for Recombinant Antibody Library Synthesis: An Advanced Source for Immunoglobulins in Environmental Analysis. In: Hock, B., Barceló, D., Cammann, K., Hansen, PD., Turner, A.P.F. (eds) Biosensors for Environmental Diagnostics. Teubner-Reihe UMWELT. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-93454-3_10
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DOI: https://doi.org/10.1007/978-3-322-93454-3_10
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