Abstract
A new method is described for the rapid identification of microorganisms in clinical samples by DNA-DNA hybridization. In this procedure, nucleic acids are labelled nonradioisotopically in the sample, which is then incubated with a panel of immobilized unlabelled DNAs from different organisms as probes. The sample labelling is a photochemical reaction between the nucleic acids in cell lysates and an isopsoralen derivative covalently linked to a biotin moiety for detection. Results are given of the application of the procedure to the identification of pathogens in clinical specimens of urine and cerebrospinal fluid.
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© 1989 Springer Fachmedien Wiesbaden
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Dattagupta, N., Huguenel, E., Rae, P., Crothers, D. (1989). Nucleic Acid Hybridization: A Rapid Method for the Diagnosis of Infectious Diseases. In: Jackson, G.G., Schlumberger, H.D., Zeiler, H.J. (eds) Perspectives in Antiinfective Therapy. Vieweg+Teubner Verlag, Wiesbaden. https://doi.org/10.1007/978-3-322-86064-4_31
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DOI: https://doi.org/10.1007/978-3-322-86064-4_31
Publisher Name: Vieweg+Teubner Verlag, Wiesbaden
Print ISBN: 978-3-528-07979-6
Online ISBN: 978-3-322-86064-4
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