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Analysis of Carbohydrates by Capillary Electrophoresis pp 9–27Cite as

Capillary electrophoresis, instrumentation and modes

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Part of the book series: CE-Series ((CHROM,volume 3))

Abstract

The success of open tube capillaries over packed columns in gas chromatography (GC) initiated the development of open tubular liquid chromatography (OTLC) in the late 70ties, in an attempt to further increase the performance of high performance liquid chromatography (HPLC) [1]. Theoretical analysis of the chromatographic process clearly showed, that miniaturization of the separation column dimensions will lead to decreased band broadening and therefore to better and/or faster analysis. However, diffusion constants are 2 to 3 orders of magnitudes smaller in liquids compared to gaseous system. In combination with the Poiseuille flow profile of the pressure generated flow, which results in fast longitudinal transport in the middle of the column and slow transport near the column wall, this leads to a relatively large diffusion controlled contribution to band broadening. Consequendy, liquid separation systems did not show the same performance increase when switching from packed columns to open column operation compared to GC systems. Theoretical calculations indicated that only with extremely small capillaries of 10 μm inner diameter (ID) or less open capillary operation in liquids could outperform packed columns.

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Authors
  1. Aran Paulus
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  2. Antje Klockow-Beck
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© 1999 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH, Braunschweig/Wiesbaden

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Paulus, A., Klockow-Beck, A. (1999). Capillary electrophoresis, instrumentation and modes. In: Analysis of Carbohydrates by Capillary Electrophoresis. Chromatographia CE-Series, vol 3. Vieweg+Teubner Verlag. https://doi.org/10.1007/978-3-322-85020-1_2

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  • DOI: https://doi.org/10.1007/978-3-322-85020-1_2

  • Publisher Name: Vieweg+Teubner Verlag

  • Print ISBN: 978-3-322-85022-5

  • Online ISBN: 978-3-322-85020-1

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