Genomic Interventions to Improve Resilience of Pigeonpea in Changing Climate

  • Abhishek BohraEmail author
  • Shalini Pareek
  • Mitchell Jones
  • Uday C. Jha
  • SJ Satheesh Naik
  • Mayank Kaashyap
  • Prakash G. Patil
  • Alok Kumar Maurya
  • Rachit Saxena
  • Rajeev K. Varshney


Pigeonpea is an important food legume crop for rainfed agriculture in developing countries, particularly in India. Productivity gains in pigeonpea have remained static, and the challenge of improving pigeonpea yield is further aggravated by increasingly uncertain climatic conditions. Improved pigeonpea cultivars with favourable traits, allowing them to cope with climatic adversities, are urgently required. Modern genomic technologies have the potential to rapidly improve breeding traits that confer resistance to biotic and abiotic stresses. Recent advances in pigeonpea genomics have led to the development of large-scale genomic tools to accelerate breeding programs. Availability of high-density genotyping assays and high-throughput phenotyping platforms motivate researchers to adopt new breeding techniques like genomic selection (GS) for improving complex traits. Accurate GS predictions inferred from multilocation and multiyear data sets also open new avenues for ‘remote breeding’ which is very much required to achieve genotype selection for future climates. Speed breeding pigeonpea with deployment of rapid generation advancement (RGA) technologies will improve our capacity to breed cultivars endowed with resilient traits. Once such climate-resilient cultivars are in place, their rapid dissemination to farmer’s fields will be required to witness the real impact. Equally important will be the acceleration of varietal turnover to keep pace with the unpredictably changing climatic conditions so that cultivars are constantly optimized for the climatic conditions at any given time.


Pigeonpea Resilience Gene Hybrid Sequencing QTL 


  1. Ae N, Arihara J, Okada K, Yoshihara T, Johansen C (1990) Phosphorus uptake by pigeon pea and its role in cropping systems of the Indian subcontinent. Science 27:477–480CrossRefGoogle Scholar
  2. Agarwal G, Garg V, Kudapa H, Doddamani D, Pazhamala LT et al (2016) Genome-wide dissection of AP2/ERF and HSP90 gene families in five legumes and expression profiles in chickpea and pigeonpea. Plant Biotechnol J 14:1563–1577CrossRefPubMedPubMedCentralGoogle Scholar
  3. Ahiabor BD, Hirata H (1994) Characteristic responses of three tropical legumes to the inoculation of two species of VAM fungi in Andosol soils with different fertilities. Mycorrhiza 5(1):63–70CrossRefGoogle Scholar
  4. Ainsworth EA, Long SP (2005) What have we learned from 15 years of free-air CO2 enrichment (FACE)? A meta-analytic review of the responses of photosynthesis, canopy properties and plant production to rising CO2. New Phytol 165:351–3702CrossRefPubMedGoogle Scholar
  5. Arora S, Mahato AK, Singh S, Mandal P, Bhutani S, Dutta S, Kumawat G, Singh BP, Chaudhary AK, Yadav R, Gaikwad K, Sevanthi AM, Datta S, Raje RS, Sharma TR, Singh NK (2017) A high-density intraspecific SNP linkage map of pigeonpea (Cajanas cajan L. Millsp.). PLoS One 12(6):e0179747Google Scholar
  6. Atlin G, Cairns GE, Das B (2017) Rapid breeding and varietal replacement are critical to adaptation of cropping systems in the developing world to climate change. Glob Food Sec 12:31–37CrossRefPubMedPubMedCentralGoogle Scholar
  7. Asha latha KV, Gopinath M, Bhat ARS (2012) Impact of climate change on rainfed agriculture in India: a case study of Dharwad. Int J Environ Sci Dev 3(4):368–371Google Scholar
  8. Bansal R, Srivastava JP (2015) Effect of waterlogging on photosynthetic and biochemical parameters in pigeonpea. Russ J Plant Physiol 62(3):322–327CrossRefGoogle Scholar
  9. Battana S, Gopal GR (2014) Antioxidative enzyme responses under single and combined effect of water and heavy metal stress in two pigeon pea cultivars. J Sci Innov Res 3(1):72–80Google Scholar
  10. Birthal P, Khan MT, Negi DS, Agarwal S (2014) Impact of climate change on yields of major food crops in India: Implications for food security. Agri Econ Res Rev 27(2):145–155CrossRefGoogle Scholar
  11. Bohra A, Mallikarjuna N, Saxena KB, Upadhyaya H, Vales I, Varshney RK (2010) Harnessing the potential of crop wild relatives through genomics tools for pigeonpea improvement. J Plant Biol 37:83–98Google Scholar
  12. Bohra A, Dubey A, Saxena RK, Penmetsa RV, Poornima KN, Kumar N et al (2011) Analysis of BAC-end sequences (BESs) and development of BES-SSR markers for genetic mapping and hybrid purity assessment in pigeonpea (Cajanus spp.). BMC Plant Biol 11:56Google Scholar
  13. Bohra A, Saxena RK, Gnanesh BN, Saxena KB, Byregowda M, Rathore A, KaviKishor PB, Cook DR, Varshney RK (2012) An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populations. Theor Appl Genet 125:1325–1338CrossRefPubMedPubMedCentralGoogle Scholar
  14. Bohra A, Saxena RK, Saxena KB, Sameerkumar CV, Varshney RK (2014) Advances in pigeonpea genomics. In: Gupta S, Nadarajan N, Sen Gupta D (eds) Legumes in the omics era. Springer, New York, Heidelberg, London, pp 95–110CrossRefGoogle Scholar
  15. Bohra A, Singh NP (2015) Whole genome sequences in pulse crops: a global community resource to expedite translational genomics and knowledge-based crop improvement. Biotechnol Lett 37:1529–1539CrossRefPubMedGoogle Scholar
  16. Bohra A, Singh IP, Yadav AK, Pathak A, Soren KR, Chaturvedi SK et al (2015) Utility of informative SSR markers in the molecular characterization of cytoplasmic genetic male sterility-based hybrid and its parents in pigeonpea. Natl Acad Sci Lett 38:13–19CrossRefGoogle Scholar
  17. Bohra A, Jha UC, Adhimoolam P, Bisht D, Singh NP (2016) Cytoplasmic male sterility (CMS) in hybrid breeding in field crops. Plant Cell Rep 35:967–993CrossRefPubMedGoogle Scholar
  18. Bohra A, Jha A, Singh IP, Pandey G, Pareek S, Basu PS, Chaturvedi SK, Singh NP (2017a) Novel CMS lines in pigeonpea [Cajanus cajan (L.) Millspaugh] derived from cytoplasmic substitutions, their effective restoration and deployment in hybrid breeding. Crop J 5:89–94CrossRefGoogle Scholar
  19. Bohra A, Pareek S, Jha R, Saxena RK, Singh IP, Pandey G et al (2017b) Modern genomic tools for pigeonpea improvement: status and prospects. In: Varshney RK, Saxena RK, Scott J (eds) The pigeonpea genome. Springer, Cham, pp 41–54Google Scholar
  20. Bohra A, Jha R, Pandey G, Patil PG, Saxena RK, Singh IP, Singh D, Mishra RK, Mishra A, Singh F, Varshney RK, Singh NP (2017c) New hypervariable SSR markers for diversity analysis, hybrid purity testing and trait mapping in Pigeonpea [Cajanus cajan (L.) Millspaugh]. Front Plant Sci 8:1–15CrossRefGoogle Scholar
  21. Chauhan JS, Singh BB, Gupta S (2016) Enhancing pulses production in India through improving seed and variety replacement rates. Indian J Genet 76:1–10Google Scholar
  22. Danekar P, Tyagi A, Mahto A, Krishna KG, Singh A, Raje RS, Gaikwad K, Singh NK (2014) Genome wide characterization of Hsp 100 family genes from pigeonpea. Indian J Genet 74(3):325–334Google Scholar
  23. Dar WD, Gowda CLL (2013) Declining agricultural productivity and global food security. J Crop Improv 27(2):242–254Google Scholar
  24. Daryanto S, Wang L, Jacinthe P-A (2015) Global synthesis of drought effects on food legume production. PLoS ONE 10(6):e0127401CrossRefPubMedPubMedCentralGoogle Scholar
  25. Das A, Datta S, Sujayan GK, Kumar M, Kumar A, Arpan S et al (2016) Expression of chimeric Bt gene, Cry1Aabc in transgenic pigeonpea (cv. Asha) confers resistance to gram pod borer (Helicoverpa armigera Hubner.) Plant Cell Tiss Org Cult 127(3):705–715Google Scholar
  26. Datta D, Bohra A, Satheesh Naik SJ et al (2017) Change in leaf pigment and root capacitance are effective indicators for waterlogging tolerance in pigeonpea. In: National symposium on pulses for nutritional security and agricultural sustainability, December 2–4, 2017. ICAR-Indian Institute of Pulses Research, Kanpur, Uttar Pradesh, IndiaGoogle Scholar
  27. Devendra C (2012) Climate change threats and effects: challenges for agriculture and food security. ASM series on climate change, pp 1–66Google Scholar
  28. Dubey A, Farmer A, Schlueter J, Cannon SB, Abernathy B, Tuteja R et al (2011) Defining the transcriptome assembly and its use for genome dynamics and transcriptome profiling studies in pigeonpea (Cajanus cajan L.) DNA Res 18:153–164Google Scholar
  29. Duhan S, Sheokand S, Kumari A, Sharma N (2017) Independent and interactive effects of waterlogging and salinity on carbohydrate metabolism and root anatomy in pigeonpea genotypes at different growth stages. Indian J Agri Res 51(3):197–205Google Scholar
  30. Dutta S, Kumawat G, Singh BP, Gupta DK, Singh S, Dogra V et al (2011) Development of genic-SSR markers by deep transcriptome sequencing in pigeonpea [Cajanus cajan (L.) Millspaugh]. BMC Plant Biol 11:17Google Scholar
  31. Emefiene ME, Salaudeen AB, Yaroson AY (2013) The use of pigeon pea (Cajanus cajan) for drought mitigation in Nigeria. Intl Lett Nat Sci 1:6–16CrossRefGoogle Scholar
  32. Flower DJ, Ludlow MM (1986) Contribution of osmotic adjustment to the dehydration tolerance of water-stressed pigeonpea (Cajanus cajan (L.) Millsp.) leaves. Plant Cell Environ 9:33–40Google Scholar
  33. Garrett KA, Dendy SP, Frank EE, Rouse MN, Travers SE (2006) Climate change effects on plant disease: genomes to ecosystems. Annu Rev Phytopathol 44:489–509CrossRefPubMedGoogle Scholar
  34. Gautam HR, Bhardwaj ML, Kumar R (2013) Climate change and its impact on plant diseases. Curr Sci 105(12)Google Scholar
  35. Geddam SB, Raje RS, Prabhu KV et al (2014) Validation of QTLs for earliness and plant type traits in pigeonpea (Cajanus cajan (L.) Millsp.). Indian J Genet 74:471–477Google Scholar
  36. Geetha N, Venkatachalam P, Lakshmi Sita G (1999) Agrobacterium mediated genetic transformation of pigeon pea (Cajanus cajan L.) and development of transgenic plants via direct organogenesis. Plant Biotechnol 16:213–218CrossRefGoogle Scholar
  37. Ghosh G, Ganguly S, Purohit A, Chaudhuri RK, Das S, Chakraborti D (2017) Transgenic pigeonpea events expressing Cry1Ac and Cry2Aa exhibit resistance to Helicoverpa armigera. Plant Cell Rep 36:1037–1051CrossRefPubMedGoogle Scholar
  38. Gnanesh BN, Bohra A, Sharma M et al (2011) Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Field Crops Res 123:53–61Google Scholar
  39. Gwata E (2012) The potential of pigeonpea (Cajanus cajan) for producing important components of renewable energy and agricultural products. Geophys Res Abstr 14, EGU2012-1857Google Scholar
  40. Haussmann BIG, Rattunde HF, Weltzien-Rattunde E, Traore PSC, vom Brocke K, Parzies HK (2012) Breeding strategies for adaptation of pearl millet and sorghum to climate variability and change in West Africa. J Agron Crop Sci 198:327–339CrossRefGoogle Scholar
  41. Hetherington SE, He J, Smillie RM (1989) Photoinhibition at low temperature in chilling-sensitive and -resistant plants. Plant Physiol 90:1609–1615CrossRefPubMedPubMedCentralGoogle Scholar
  42. Hingane AJ, Saxena KB, Patil SB, Sultana R, Srikanth S, Mallikarjuna N, Vijaykumar R, Kumar CVS (2015) Mechanism of water-logging tolerance in pigeonpea. Indian J Genet 75(2):208–214Google Scholar
  43. Kassa MT, Penmetsa RV, Carrasquilla-Garcia N et al (2012) Genetic patterns of domestication in pigeonpea (Cajanus cajan (L.) Millsp.) and wild Cajanus relatives. PLoS One 7:e39563Google Scholar
  44. Kumawat G, Raje RS, Bhutani S et al (2012) Molecular mapping of QTLs for plant type and earliness traits in pigeonpea (Cajanus cajan L. Millsp.). BMC Genet 13:84Google Scholar
  45. Kaur A, Sharma M, Sharma C, Kaur H, Kaur N, Sharma S, Arora R, Singh I, Sandhu JS (2016) Pod borer resistant transgenic pigeon pea (Cajanus cajan L.) expressing cry1Ac transgene generated through simplified Agrobacterium transformation of pricked embryo axes. Plant Cell Tiss Org Cult 127:717–727CrossRefGoogle Scholar
  46. Krishna GG, Reddy PS, Ramteke PW, Rambabu P, Tawar KB, Bhattacharya P (2011) Agrobacterium-mediated genetic transformation of pigeon pea [Cajanus cajan (L.) Millsp.] for resistance to legume pod borer Helicoverpa armigera. J Crop Sci Biotechnol 14:197–204CrossRefGoogle Scholar
  47. Krishnamurthy L, Upadhyaya HD, Saxena KB, Vadez V (2012) Variation for temporary waterlogging response within the mini core pigeonpea germplasm. J Agri Sci 150(3):357–364CrossRefGoogle Scholar
  48. Kudapa H, Bharti AK, Cannon SB, Farmerb AD, Mulaosmanovic B, Kramerb R et al (2012) A comprehensive transcriptome assembly of pigeonpea (Cajanus cajan l.) using Sanger and second-generation sequencing platforms Mol Plant 5(5):1020–1028Google Scholar
  49. Kumar A, Sharma P (2013) Impact of climate variation on agricultural productivity and food security in rural India. Economics discussion papers no. 2013-43Google Scholar
  50. Kumar RR, Karjol K, Naik GR (2011) Variation of sensitivity to drought stress in pigeon pea (Cajanus cajan [L.] Millsp) cultivars during seed germination and early seedling growth. World J Sci Technol 1(1):11–18Google Scholar
  51. Kumar RR, Yadav S, Joshi S, Bhandare PP, Patil VK, Kulkarni PB, Sonkawade S, Naik GR (2014) Identification and validation of expressed sequence tags from pigeonpea (Cajanus cajan L.) root. Intl J Plant Genom 2014:651912Google Scholar
  52. Kumar SM, Kumar BK, Sharma KK, Devi P (2004) Genetic transformation of pigeonpea with rice chitinase gene. Plant Breed 123:485–489CrossRefGoogle Scholar
  53. Kumar A, Nath P (2005) Insect pests of early pigeon-pea in relation to weather parameters. Ann Plant Protec Sci 13(1):23–26Google Scholar
  54. Kumar V, Khan AW, Saxena RK, Garg V, Varshney RK (2016) First‐generation HapMap in Cajanus spp. reveals untapped variations in parental lines of mapping populations. Plant Biotechnol J 14(8):1673–1681Google Scholar
  55. Kumar P, Sharma V, Atmaram CK, Singh B (2017) Regulated partitioning of fixed carbon (14C), sodium (Na+), potassium (K+) and glycine betaine determined salinity stress tolerance of gamma irradiated pigeonpea [Cajanus cajan (L.) Millsp]. Environ Sci Pollut Res 24:7285–7297CrossRefGoogle Scholar
  56. Kumutha D, Ezhilmathi K, Sairam RK, Srivastava GC, Deshmukh PS, Meena RC (2009) Waterlogging induced oxidative stress and antioxidant activity in pigeonpea genotypes. Biol Plant 53(1):75–84CrossRefGoogle Scholar
  57. Kumutha D, Sairam RK, Ezhilmathi K, Chinnusamy V, Meena RC (2008) Effect of waterlogging on carbohydrate metabolism in pigeon pea (Cajanus cajan L.): Upregulation of sucrose synthase and alcohol dehydrogenase. Plant Sci 175(5):706–716Google Scholar
  58. Lal R, Wilson GF, Okigbo BN (1978) No-till farming after various grasses and leguminous cover crops in tropical alfisol. I. Crop performance. Field Crops Res 1:71–84CrossRefGoogle Scholar
  59. Lawrence PK, Koundal KR (2001) Agrobacterium tumefaciens mediated transformation of pigeon pea (Cajanus cajan L. Millsp.) and molecular analysis of regenerated plants. Curr Sci 80:1428–1432Google Scholar
  60. Lopez FB, Johansen C, Chauhan YS (1996) Effects of timing of drought stress on phenology, yield and yield components of short-duration pigeonpea. J Agron Crop Sci 177(5):311–320CrossRefGoogle Scholar
  61. Lopez FB, Setter TM, McDavid CR (1987) Carbon dioxide and light responses of photosynthesis in cowpea and pigeonpea during water deficit and recovery. Plant Physiol 85(4):990–995CrossRefPubMedPubMedCentralGoogle Scholar
  62. Maibam A, Tyagi A, Satheesh V, Mahato AK, Jain N, Raje RS, Rao AR, Gaikwad K, Singh NK (2015) Genome-wide identification and characterization of heat shock factor genes from pigeonpea (Cajanus cajan). Mol Plant Breed 6(7):1–11Google Scholar
  63. Manickavelu A, Hattori T, Yamaoka S, Yoshimura K, Kondou Y, Onogi A et al (2017) Genetic nature of elemental contents in wheat grains and its genomic prediction: toward the effective use of wheat landraces from Afghanistan. PLoS ONE 12:e0169416CrossRefPubMedPubMedCentralGoogle Scholar
  64. Marle PS, Hillocks RJ (1993) The role of phytoalexins in resistance to fusarium wilt in pigeon pea (Cajanus cajan). Plant Pathol 42:212–218CrossRefGoogle Scholar
  65. Marsh LE, Baptiste R, Marsh DB, Trinklein D, Kremer RJ (2006) Temperature effects on bradyrhizobium spp. growth and symbiotic effectiveness with pigeonpea and cowpea. J Plant Nutr 29:331–346CrossRefGoogle Scholar
  66. Mathukumalli SR, Dammua M, Sengottaiyan V, Ongolu S, Birada AK, Kondrua VR, Karlapudia S, Bellapukonda MKR, Chitiprolu RRA, Cherukumalli SR (2016) Prediction of Helicoverpa armigera Hubner on pigeonpea during future climate change periods using MarkSim multimodel data. Agri For Meteorol 228:130–138CrossRefGoogle Scholar
  67. Mellacheruvu S, Tamirisa S, Vudem DR, Khareedu VR (2016) Pigeonpea hybrid-proline rich protein (CcHyPRP) confers biotic and abiotic stress tolerance in transgenic rice. Front Plant Sci 6:1167CrossRefPubMedPubMedCentralGoogle Scholar
  68. Mir RR, Saxena RK, Saxena K, Upadhyaya HD, Kilian A, Cook DR, Varshney RK (2012) Whole-genome scanning for mapping determinacy in pigeonpea (Cajanus spp.) Plant Breed 132(5):472–478Google Scholar
  69. Mligo JK, Craufurd PQ (2005) Adaptation and yield of pigeonpea in different environments in Tanzania. Field Crops Res 94(1):43–53CrossRefGoogle Scholar
  70. Myaka FM, Sakala WD, Adu-Gyamfi JJ, Kamalongo D, Ngwira A, Odgaard R, Nielsen RE, Høgh-Jensen H (2006) Yields and accumulations of N and P in farmer-managed intercrops of maize–pigeonpea in semi-arid Africa. Plant Soil 285(1–2):207–220CrossRefGoogle Scholar
  71. Nam NH, Chauhan YS, Johansen C (2001) Effect of timing of drought stress on growth and grain yield of extra-short-duration pigeonpea lines. J Agri Sci 136(2):179–189CrossRefGoogle Scholar
  72. Ogata S, Adu-Gyamfi S, Fujita K (1988) Effect of phosphorus and pH on dry matter production, dinitrogen fixation and critical phosphorus concentration in pigeon pea (Cajanus cajan (L) millsp.). Soil Sci Plant Nutr 34(1):55–64Google Scholar
  73. Okiror MA (1986) Breeding for resistance to Fusarium wilt of pigeonpea (Cajanm cajan (L.) Millsp.) in Kenya. PhD thesis, University of Nairobi, 202 pGoogle Scholar
  74. Onim JFM, Semenye PP, Fitzhugh HA, Mathuva M (1985) Research on feed resources for small ruminants on smallholder farms in Western Kenya. In: Kategile JA, Said AN, Dzowela BH (eds) Animal feed resources for small-scale livestock producers. Proceedings of the 2nd PANESA workshop, held in Nairobi, KenyaGoogle Scholar
  75. Oswald A, Ransom JK (2001) Striga control and improved farm productivity using crop rotation. Crop Protec 20(2):113–120CrossRefGoogle Scholar
  76. Pande S, Sharma M (2011) Climate change and changing scenario of plant diseases in semi arid tropics, plant pathology in India: Vision 2030. Indian Phytopathological Society, New Delhi, pp 128–131Google Scholar
  77. Parent C, Capelli N, Berger A, Crèvecoeur M, Dat JF (2008) An overview of plant responses to soil waterlogging. Plant Stress 2:20–27Google Scholar
  78. Patil PG, Dubey J, Bohra A, Mishra RK, Saabale PR, Das A, Rathore M, Singh NP (2017a) Association mapping to discover significant marker-trait associations for resistance against Fusarium wilt variant 2 in pigeonpea [Cajanus cajan (L.) Millspaugh] using SSR markers. J Appl Genet 58:307–319Google Scholar
  79. Patil PG, Bohra A, Dubey J, Saabale PR, Mishra RK, Pandey G, Das A, Rathore M, Singh F, Singh NP (2017b) Genetic analysis and molecular resistance to race 2 of Fusarium wilt in pigeonpea [Cajanus cajan (L.) Millsp.]. Crop Prot 100:117–123Google Scholar
  80. Patil PG, Bohra A, Satheesh Naik SJ, et al (2018) Validation of QTLs for plant ideotype, earliness and growth habit traits in pigeonpea (Cajanus cajan Millsp.). Physiol Mol Biol Plants 24:1245–1259Google Scholar
  81. Pathania M, War AR, Munghate RS, Nagraja T, Sharma HC (2014) Effects of climatic factors on pest incidence in pigeonpea. In: National conference on pulses: challenges and opportunities under changing climate scenario, 29th Sept–1st Oct 2014Google Scholar
  82. Prasad JVNS, Srinivasa Rao CH, Ravichandra K, Naga Jyothi CH, Prasad Babu MBB, Babu VR et al (2015) Green house gas fluxes from rainfed sorghum (Sorghum bicolour) and pigeonpea (Cajanus cajan)—Interactive effects of rainfall and temperature. J Agrometeorol 17(1):17–22Google Scholar
  83. Prasad YG, Bambawale OM (2010) Effects of climate change on natural control of insect pests. Indian J Dryland Agric Res Dev 25(2):1–12Google Scholar
  84. Pritchard SG, Rogers HH, Prior SA, Peterson CM (1999) Elevated CO2 and plant structure: a review. Glob Change Biol 5(7):807–837CrossRefGoogle Scholar
  85. Priyanka B, Sekhar K, Reddy VD, Rao KV (2010a) Expression of pigeonpea hybrid-proline-rich protein encoding gene (CcHyPRP) in yeast and Arabidopsis affords multiple abiotic stress tolerance. Plant Biotechnol J 8:76–87CrossRefPubMedGoogle Scholar
  86. Priyanka B, Sekhar K, Sunita T, Reddy VD, Rao KV (2010b) Characterization of expressed sequence tags (ESTs) of pigeonpea (Cajanus cajan L.) and functional validation of selected genes for abiotic stress tolerance in Arabidopsis thaliana. Mol Genet Genom 283:273–287CrossRefGoogle Scholar
  87. Raju NL, Gnanesh BN, Lekha P, Jayashree B, Pande S, Hiremath PJ, Byregowda M, Singh NK, Varshney RK (2010) The first set of EST resource for gene discovery and marker development in pigeonpea (Cajanus cajan L.). BMC Plant Biol 10:45Google Scholar
  88. Ramu SV, Rohini S, Keshavareddy G, Gowri Neelima M, Shanmugam NB, Kumar ARV, Sarangi SK, Kumar PA, Udayakumar M (2012) Expression of a synthetic cry1AcF gene in transgenic pigeon pea confers resistance to Helicoverpa armigera. J Appl Entomo1 36:675–687Google Scholar
  89. Rao SC, Coleman SW, Mayeux HS (2002) Forage production and nutritive value of selected pigeonpea ecotypes in the southern Great Plains. Crop Sci. 42(4):1259–1263CrossRefGoogle Scholar
  90. Ray DK, Ramankutty N, Mueller ND, West PC, Foley JA (2012) Recent patterns of crop yield growth and stagnation. Nat Commun 3:1293CrossRefPubMedGoogle Scholar
  91. Ray DK, Gerber JS, MacDonald GK, West PC (2015) Climate variation explains a third of global crop yield variability. Nat Commun 6:5989CrossRefPubMedPubMedCentralGoogle Scholar
  92. Saha S, Sehgal VK, Nagarajan S, Pal M (2012) Impact of elevated atmospheric CO2 on radiation utilization and related plant biophysical properties in pigeon pea (Cajanus cajan L.). Agri For Meteorol 158–159:63–70CrossRefGoogle Scholar
  93. Sairam RK, Kumutha D, Ezhilmathi K, Chinnusamy V, Meena RC (2009) Waterlogging induced oxidative stress and antioxidant enzyme activities in pigeon pea. Biol Plant 53(3):493–504CrossRefGoogle Scholar
  94. Sakala WD, Cadisch G, Giller KE (2000) Interactions between residues of maize and pigeonpea and mineral N fertilizers during decomposition and N mineralization. Soil Biol Biochem 32(5):679–688CrossRefGoogle Scholar
  95. Saxena KB, Choudhary AK, Srivastava RK, Bohra A, Saxena RK, Varshney RK (2019) Origin of early maturing pigeonpea germplasm and its impact on adaptation and cropping systems. Plant Breed.
  96. Saxena KB, Kumar RV, Tikle AN et al (2013) ICPH2671—the world’s first commercial food legume hybrid. Plant Breed 132:479–485Google Scholar
  97. Saxena KB, Singh IP, Bohra A et al (2015) Strategies for breeding, production, and promotion of pigeonpea hybrids in India. J Food Legume 28:190–198Google Scholar
  98. Saxena KB, Saxena RK, Varshney RK (2017a) Use of immature seed germination and single seed descent for rapid genetic gains in pigeonpea. Plant Breed 136:954–957CrossRefGoogle Scholar
  99. Saxena RK, Kale SM, Kumar V, Parupalli S, Joshi S, Singh VK, Garg V, Das RR, Sharma M, Yamini KN, Ghanta A, Rathore A, Sameer Kumar CV, Saxena KB, Varshney RK (2017b) Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea. Sci Rep 7:1813CrossRefPubMedPubMedCentralGoogle Scholar
  100. Saxena RK, Singh VK, Kale SM, Tathineni R, Parupalli S, Kumar V, Garg V, Das RR, Sharma M, Yamini KN, Muniswamy S, Ghanta A, Rathore A, Sameer Kumar CV, Saxena KB, Kavi Kishor PB, Varshney RK (2017c) Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea. Sci Rep 7:1911CrossRefPubMedPubMedCentralGoogle Scholar
  101. Saxena RK, Penmetsa RV, Upadhyaya HD, Kumar A, Carrasquilla-Garcia N, Schlueter JA, Farmer A, Whaley AM, Sarma BK, May GD, Cook DR, Varshney RK (2012) Large-scale development of cost-effective single-nucleotide polymorphism marker assays for genetic mapping in pigeonpea and comparative mapping in legumes. DNA Res 19:449–461CrossRefPubMedPubMedCentralGoogle Scholar
  102. Saxena RK, Patel K, Sameer Kumar CV, Tyagi K, Saxena KB, Varshney RK (2018) Molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea (Cajanus cajan (L.) Millsp.). Theor Appl Genet.
  103. Setter TL, Belford R (1990) Waterlogging—how it reduces plant growth and how plants can overcome its effects. J Agri 31:51–55Google Scholar
  104. Setter TL, Ellis M, Laureles EV, Ella ES, Senadhira D, Mishra SB, Sarkarung S, Datta S (1997) Physiology and genetics of submergence tolerance in rice. Ann Bot 79:67–77CrossRefGoogle Scholar
  105. Sekhar K, Priyanka B, Reddy VD, Rao KV (2010) Isolation and characterization of a pigeonpea cyclophilin (CcCYP) gene, and its over-expression in Arabidopsis confers multiple abiotic stress tolerance. Plant Cell Environ 33:1324–1338PubMedGoogle Scholar
  106. Sharma HC (2016) Climate change vis-a-vis pest management. In: Conference on national priorities in plant health management, 4–5 Feb 2016, Tirupati, IndiaGoogle Scholar
  107. Sharma HC, Pampapathy G, Reddy LJ (2003) Wild relatives of pigeonpea as a source of resistance to the pod fly (Melanagromyza obtusa Malloch) and pod wasp (Tanaostigmodes cajaninae La Salle). Genet Resour Crop Evol 50(8):817–824CrossRefGoogle Scholar
  108. Sharma KK, Lavanya M, Anjaiah V (2006) Agrobacterium-mediated production of transgenic pigeonpea (Cajanus cajan L. Millsp.) expressing the synthetic BT cry1Ab gene. In Vitro Cell Dev Biol Plant 42(2):165–173Google Scholar
  109. Sharma SB, Mohiuddin M, Jain KC, Remanandan P (1994) Reaction of pigeonpea cultivars and germplasm accessions to the root-knot nematode, Meloidogyne javanica. J Nematol 26(4S):644–652PubMedPubMedCentralGoogle Scholar
  110. Shibairo SI, Nyabundi JO, Otieno W (1995) Effects of temperature on germination of seeds of three pigeonpea (Cajanus cajan) genotypes. Discov Innov 7(3):283–287Google Scholar
  111. Singh IP, Bohra A, Singh F (2016a) An overview of varietal development programme of pigeonpea in India. Legume Perspect 11:37–40Google Scholar
  112. Singh VK, Khan AW, Saxena RK et al (2016b) Next-generation sequencing for identification of candidate genes for Fusarium wilt and sterility mosaic disease in pigeonpea (Cajanus cajan). Plant Biotechnol J 14:1183–1194Google Scholar
  113. Singh VK, Khan AW, Saxena RK et al (2017) Indel-seq: a fast-forward genetics approach for identification of trait-associated putative candidate genomic regions and its application in pigeonpea (Cajanus cajan). Plant Biotechnol J 15:906–914Google Scholar
  114. Singh K, Sharma SP, Singh TK, Singh Y (1986) Effect of waterlogging on growth, yield and nutrient concentration of black gram and green gram under subtropical condition of Varanasi. Ann Agri Res 7:169–177Google Scholar
  115. Sinha P, Pazhamala LT, Singh VK, Saxena RK, Krishnamurthy L, Azam S, Khan AW and Varshney RK (2016) Identification and validation of selected universal stress protein domain containing drought-responsive genes in pigeonpea (Cajanus cajan L.). Front Plant Sci 6:1065Google Scholar
  116. Sinha P, Saxena KB, Saxena RK et al (2015a) Association of nad7a gene with cytoplasmic male sterility in pigeonpea. Plant Genome 8:1–12Google Scholar
  117. Sinha P, Saxena RK, Singh VK, Krishnamurthy L, Varshney RK (2015b) Selection and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under heat and salt stress conditions. Front Plant Sci 6:1071PubMedPubMedCentralGoogle Scholar
  118. Sinha P, Singh VK, Suryanarayana V, Krishnamurthy L, Saxena RK, Varshney RK (2015c) Evaluation and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under drought stress conditions. PLoS ONE 10:e0122847Google Scholar
  119. Singh NK, Gupta DK, Jayaswal PK, Mahato AK et al (2012) The first draft of the pigeon pea genome sequence. J Plant Biochem Biotechnol 21:98–112CrossRefPubMedGoogle Scholar
  120. Sogbedji JM, van Es HM, Agbeko KL (2006) Cover cropping and nutrient management strategies for maize production in western Africa. Agron J 98:883–889CrossRefGoogle Scholar
  121. Sreeharsha RV, Reddy AR (2015) Dynamics of vegetative and reproductive growth patterns in Pigeonpea (Cajanus cajan L.) grown under elevated CO2. Proced Environ Sci 29:147–148CrossRefGoogle Scholar
  122. Sreeharsha RV, Sekhar KM, Reddy AR (2015) Delayed flowering is associated with lack of photosynthetic acclimation in pigeon pea (Cajanus cajan L.) grown under elevated CO2. Plant Sci 231:82–93CrossRefPubMedGoogle Scholar
  123. Sri ND, Mohan MM, Mahesh K, Raghu K, Rao SSR (2016) Amelioration of aluminium toxicity in pigeon pea [Cajanus cajan (l.) millsp.] plant by 24-epibrassinolide. Amer J Plant Sci 7:1618–1628CrossRefGoogle Scholar
  124. Srivastava JP, Singh P, Singh VP, Bansal R (2010) Effect of waterlogging on carbon exchange rate, stomatal conductance and mineral nutrients status in maize and pigeonpea. Plant Stress 4(1):94–99Google Scholar
  125. Srivastava N, Vadez V, Upadhyaya HD, Saxena KB (2006) Screening for intra and inter specific variability for salinity tolerance in pigeonpea (Cajanus cajan) and its related wild species. SAT J 2(1)Google Scholar
  126. Subbarao GV, Chauhan YS, Johansen C (2000) Patterns of osmotic adjustment in pigeonpea—its importance as a mechanism of drought resistance. Eur J Agron 12(3–4):239–249CrossRefGoogle Scholar
  127. Subbarao GV, Noriharu A, Otani T (1997) Genetic variation in acquisition, and utilization of phosphorus from iron-bound phosphorus in pigeonpea. Soil Sci Plant Nutr 43(3):511–519CrossRefGoogle Scholar
  128. Subbarao GY, Johansen C, Jana MK, Kumar Rao JVDK (1991) Comparative salinity responses among pigeonpea genotypes and their wild relatives. Crop Sci 3(l):415–418Google Scholar
  129. Sultana R, Vales MI, Saxena KB et al (2012) Water-logging tolerances in pigeonpea (Cajanus cajan L. Millsp.) genotypic variability and identification of tolerant genotypes. J Agric Sci:1–13Google Scholar
  130. Surekha CH, Beena MR, Arundhatia A, Singh PK, Tuli R, Dutta-Gupta A, Kirti PB (2005) Agrobacterium-mediated genetic transformation of pigeon pea (Cajanus cajan (L.) Millsp.) using embryonal segments and development of transgenic plants for resistance against Spodoptera. Plant Sci 169:1074–1080CrossRefGoogle Scholar
  131. Surekha C, Kumari KN, Aruna LV, Suneetha G, Arundhati A Kishor PBK (2014) Expression of the Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance. Plant Cell Org Cult 116: 27–36Google Scholar
  132. Rogers A, Ainsworth EA, Leakey ADB (2009) Will elevated carbon dioxide concentration amplify benefits of nitrogen fixation in legumes? Plant Physiol 151:1009–1016CrossRefPubMedPubMedCentralGoogle Scholar
  133. Takele A, McDavid CR (1995) The response of pigeon pea cultivars to short durations of waterlogging. Afr Crop Sci J 3:51–58Google Scholar
  134. Tamirisa S, Vudem DR, Khareedu VR (2014) Overexpression of pigeonpea stress-induced cold and drought regulatory gene (CcCDR) confers drought, salt, and cold tolerance in Arabidopsis. J Exp Bot 65(17):4769–4781CrossRefPubMedPubMedCentralGoogle Scholar
  135. Taub D (2010) Effects of rising atmospheric concentrations of carbon dioxide on plants. Nat Edn Knowl 3:21Google Scholar
  136. Upadhyaya HD, Gowda CLL, Sastry DVSSR (2008) Plant genetic resources management: collection, characterization, conservation and utilization. SAT J 6:1–15Google Scholar
  137. Upadhyaya HD, Reddy KN, Shivali S (2011) Pigeonpea composite collection for enhanced utilization of germplasm in crop improvement programs. Plant Genet Resour Charact Util 9:97–108Google Scholar
  138. Varshney RK, Chen W, Li Y, Bharti AK et al (2012) Draft genome sequence of pigeon pea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nat Biotechnol 30:83–89CrossRefGoogle Scholar
  139. Varshney RK, Murali Mohan S, Gaur PM et al (2013) Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics. Biotechnol Adv S0734–9750Google Scholar
  140. Varshney RK, Pandey MK, Bohra A et al (2018b) Towards sequence-based breeding in legumes in the post-genome sequencing era. Theor Appl Genet.
  141. Varshney RK, Saxena RK, Upadhyaya HD, Khan AW, Yu Y, Kim C et al (2017) Whole-genome resequencing of 292 pigeon pea accessions identifies genomic regions associated with domestication and agronomic traits. Nat Genet 49(7):1082–1088CrossRefPubMedGoogle Scholar
  142. Varshney RK, Thudi M, Pandey MK, Tardieu F, Ojiewo C, Vadez V, Whitbread AM, Siddique KHM, Nguyen HT, Carberry PS, Bergvinson D (2018a) Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy. J Exp Bot.
  143. Valenzuela HR, Smith J (2002) CTAHR sustainable agriculture green manure crops series: pigeonpea. Univ Hawaii Coop Ext Serv SA-GM-8Google Scholar
  144. Vanaja M, Reddy PR R, Lakshmi NJ, Razak SKA, Vagheera P, Archana G, Yadav SK, Maheswari M, Venkateswarlu B (2010) Response of seed yield and its components of red gram (Cajanus cajan L. Millsp.) to elevated CO2. Plant Soil Environ 56(10):458–462Google Scholar
  145. Velez-Colon R, Garrison SA (1989) Growth, maturity and flowering of pigeon peas, Cajanus cajan L Millsp., at high latitudes. J Agri Univ PR 73(3)Google Scholar
  146. Venzon M, Rosado MC, Euzebio DE, Souza B, Schoerder JH (2006) Suitability of leguminous cover crop pollens as food source for the green lacewing Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae). Neotrop Entomol 35(3):371–376CrossRefPubMedGoogle Scholar
  147. Wei Z, Luo M, Zhao C, Li C, Gu C, Wang W, Zu Y, Efferth T, Fu Y (2013) UV-induced changes of active components and antioxidant activity in postharvest pigeon pea [Cajanus cajan (L.) Millsp.] leaves. J Agri Food Chem 61(6):1165–1171Google Scholar
  148. Wellings NP, Wearing AH, Thompson JP (1991) Vesicular-arbuscular mycorrhizae (VAM) improve phosphorus and zinc nutrition and growth of pigeonpea in a Vertisol. Aust J Agri Res 42:835–845CrossRefGoogle Scholar
  149. Yadav RC (2017) Cropping practice and makeup shortfall of pulse production with reduced emission of green house gas-nitrous oxide. Arch Chem Res 1:2Google Scholar
  150. Zade D, Rao KB, Bendapudi R, D’souza M (2013) Towards resilient agriculture in a changing climate scenario. Watershed Organisation Trust 1–28Google Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Abhishek Bohra
    • 1
    Email author
  • Shalini Pareek
    • 1
  • Mitchell Jones
    • 2
  • Uday C. Jha
    • 1
  • SJ Satheesh Naik
    • 1
  • Mayank Kaashyap
    • 3
  • Prakash G. Patil
    • 1
  • Alok Kumar Maurya
    • 1
  • Rachit Saxena
    • 4
  • Rajeev K. Varshney
    • 4
    • 5
  1. 1.ICAR-Indian Institute of Pulses Research (IIPR)KanpurIndia
  2. 2.RMIT UniversityMelbourneAustralia
  3. 3.Cornell UniversityIthacaUSA
  4. 4.International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)HyderabadIndia
  5. 5.The University of Western AustraliaCrawleyAustralia

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