Systems-Level Analysis of Bacterial Regulatory Small RNA Networks
The RNA landscape of all sequenced bacteria is littered with regulatory noncoding small RNAs (sRNA). Understanding the functions of these sRNAs has lagged behind their identification, as few high-throughput approaches existed to capture sRNA interactions in vivo. Recently, methodologies have been described that allow for profiling of the sRNA interaction network facilitating systems-level analysis sRNA regulation. This chapter discusses recent advances in our understanding of sRNA function, technical advances that allow us to capture sRNA interactions in vivo, and the computational tools that allow meaningful conclusions to be drawn from these data.
KeywordsSmall regulatory RNAs Bacteria RNase E Hfq Networks sRNA interactomes
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