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Plant Tissue Culture Techniques for Breeding

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Ornamental Crops

Part of the book series: Handbook of Plant Breeding ((HBPB,volume 11))

Abstract

Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been applied successfully in many species and have been used to create plants that are disease and insect resistant and tolerant to water stress and demonstrate enhanced growth and improved yields and much more. Traditional plant breeding is enhanced with techniques such as embryo culture to rescue recalcitrant hybrids. Technologies as protoplast fusion and somaclonal variation are used to exploit existing genetic diversity or to produce new genetic variability. Once new plants are developed, micropropagation can be used to efficiently clonally propagate large numbers for the commercial market. Genetic engineering would not be possible without the involvement of in vitro plant tissue culture regeneration procedures.

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References

  • Al-Atabee JS, Power JB (1987) Plant regeneration form protoplasts of Dimorphotheca and Rudbeckia. Plant Cell Rep 6:414–416

    PubMed  CAS  Google Scholar 

  • Anderson WC (1980) Mass propagation by tissue culture. In: Principles and techniques. Agricultural research results, United States Department of Agriculture, pp 1–9

    Google Scholar 

  • Bridgen MP (1994a) A review of plant embryo culture. Hortscience 29:1243–1246

    Google Scholar 

  • Bridgen MP (1994b) Tissue culture’s potential for introducing new plants. Proceedings of the International Plant Propagators’ Society 44:595–601

    Google Scholar 

  • Chabane D, Assani A, Bouguedoura N, Haïcour R, Ducreux G (2007) Induction of callus formation from difficile date palm protoplasts by means of nurse culture. CR Biol 330:392–401

    Article  Google Scholar 

  • Cline MG (1991) Apical dominance. Bot Rev 57:318–358

    Article  Google Scholar 

  • Constabel F (1984) Fusion of protoplasts by polyethylene glycol (PEG). In: Vasil IK (ed) Cell culture and somatic cell genetics of plants: volume 1: laboratory procedures and their applications. Academic Press Inc, Orlando, Florida, pp 414–422

    Chapter  Google Scholar 

  • Davey RM, Anthony P, Patel D, Power JB (2010) Plant protoplasts: isolation, culture and plant regeneration. In: Davey RM, Anthony P (eds) Plant cell culture: essential methods. Wiley-Blackwell, West Sussex, UK, pp 153–174

    Google Scholar 

  • Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hortic 14(4):335–345

    Article  Google Scholar 

  • Desjardins Y, Hdider C, De Riek J (1995) Carbon nutrition in vitro- regulation and manipulation of carbon assimilation in micropropagated systems. In: George EF, Hall MA, De Klerk GJ (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 441–471

    Chapter  Google Scholar 

  • do Valle Rego L, Tadeu de Faria R (2001) Tissue culture in ornamental plant breeding: a review. Crop Breed Appl Biotechnol 1:283–300

    Article  Google Scholar 

  • Duquenne B, Eeckhaut T, Werbrouck S, Van Huylenbroeck J (2007) Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium. Plant Cell Tissue Org Cult 91(2):165–173

    Article  CAS  Google Scholar 

  • Eeckhaut T, Lakshmanan PS, Deryckere D, Van Bockstaele E, Van Huylenbroeck J (2013) Progress in plant protoplast research. Planta 238:991–1003

    Article  CAS  PubMed  Google Scholar 

  • Evans DA, Bravo JE, Gleba YY (1983) Somatic hybridization: fusion methods, recovery of hybrids, and genetic analysis. Int Rev Cytol 16:143–159

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  CAS  PubMed  Google Scholar 

  • George EF, Hall MA, De Klerk G-J (2008a) Plant tissue culture procedure – background. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture. Vol. 1, The background, 3rd edn. Springer, Dordrecht, pp 1–28

    Google Scholar 

  • George EF, Hall MA, De Klerk G-J (2008b) The component of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht, pp 115–173

    Google Scholar 

  • Haberlandt G (1902) Culturversuche mit isolierten pflanzenzellen, sitz-ungsb. Akad. D. wissensch. Mathermatusch-naturwissenschaftlicher c169

    Google Scholar 

  • Haslam TM, Yeung EC (2011) Zygotic embryo culture: an overview. In: Thorpe TA, Yeung EC (eds) Plant embryo culture: methods and protocols, Methods in molecular biology, vol 710. Humana Press, Totowa, pp 3–15

    Chapter  Google Scholar 

  • Hu X, Yin Y, He T (2015) Plant regeneration from protoplasts of Gentiana macrophylla pall. Using agar-pool culture. Plant Cell Tissue Organ Cult 121(2):345–351

    Article  CAS  Google Scholar 

  • Jain SM, Buiatti M, Gimelli F, Saccardo F (1998) Somaclonal variation in improving ornamental plants. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement, Current plant science and biotechnology in agriculture, vol 32. Springer, Dordrecht, pp 81–104

    Chapter  Google Scholar 

  • Johnson AA, Veilleux RE (2001) Somatic hybridization and application in plant breeding. Plant Breed Rev 20:167–225

    CAS  Google Scholar 

  • Jones AM, Shukla MR, Biswas GC, Saxena PK (2015) Protoplast-to-plant regeneration of American elm (Ulmus americana). Protoplasma 252:925–931

    Article  CAS  PubMed  Google Scholar 

  • Kaeppler SM, Kaeppler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43:179–188

    Article  CAS  PubMed  Google Scholar 

  • Kang J-M, Kojima K, Ide Y, Sasaki S (1996) Plantlet regeneration from leaf protoplasts of Populus euphratica. J For Res 1:99–102

    Article  Google Scholar 

  • Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110

    Article  CAS  PubMed  Google Scholar 

  • Kästner U, Klocke E, Abel S (2017) Regeneration of protoplasts after somatic hybridization of Hydrangea. Plant Cell Tissue Organ Cult 129:359–373

    Article  CAS  Google Scholar 

  • Klocke E, Weinzierl K, Abel S (2013) Occurrence of endophytes during Pelargonium protoplast culture. In: Schneider C (ed) Endophytes for plant protection: the state of the art. DPG Verlag, Germany, pp 94–99

    Google Scholar 

  • Knudson L (1922) Nonsymbiotic germination of orchid seeds. Bot Gaz 73:1–25

    Article  Google Scholar 

  • Krishna H, Alizadeh M, Singh D, Singh U, Chauhan N, Eftekhari M, Sadh RK (2016) Somaclonal variations and their applications in horticultural crops improvement. 3. Biotech 6(1):54. https://doi.org/10.1007/s13205-016-0389-7

    Article  Google Scholar 

  • Kyte L, Klyen J, Scoggins H, Bridgen M (2013) Plants from test tubes: an introduction to micropropagation, 4th edn. Timber Press, Portland, p 250

    Google Scholar 

  • Mii M (2001) Somatic hybridization in ornamental species. The Korean breeding society conference: Introduction pp 20–22

    Google Scholar 

  • Monnier M (1995) Culture of zygotic embryos. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 117–153

    Chapter  Google Scholar 

  • Murashige T (1974) Plant propagation through tissue cultures. Annu Rev Plant Physiol 25:135–166

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nakano M, Hoshino Y, Mii M (1996) Intergeneric somatic hybrid plantlets between Dianthus barbatus and Gypsophila paniculata obtained by electrofusion. Theor Appl Genet 92(2):170–172

    Article  CAS  PubMed  Google Scholar 

  • Navrátilová B (2004) Protoplast cultures and protoplast fusion focused on Brassicaceae – a review. Hortic Sci 31(4):140–157

    Article  Google Scholar 

  • Norstog K (1979) Embryo culture as a tool in the study of comparative and developmental morphology. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture. Ohio State University Press, Columbus, pp 179–202

    Google Scholar 

  • Ochatt SJ (1991) Requirements for plant regeneration from protoplasts of the shrubby ornamental honeysuckle, Lonicera nitida cv. Maigrun. Plant Cell Tissue Organ Cult 25:161–167

    Google Scholar 

  • Pasqual M, Soares JD, Rodrigues FA (2014) Tissue culture application for the genetic improvement of plants. Biotechnology and Plant Breeding: applications and approaches for developing improved cultivars 225:157–199

    Google Scholar 

  • Preece JE, Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer Academic Publisher/Springer, Dordrecht, pp 71–93

    Chapter  Google Scholar 

  • Rahmani MS, Shabanian N, Pijut PM (2016) Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin. Plant Cell Tissue Organ Cult 127:475–488

    Article  CAS  Google Scholar 

  • Read PE (1992) Environmental and hormonal effects in micropropagation. In: Kurata K, Kozai T (eds) Transplant production systems. Kluwer Academic Publisher/Springer, Dordrecht, pp 231–246

    Chapter  Google Scholar 

  • Sharma D, Kaur R, Kumar K (1996) Embryo rescue in plants – a review. Euphytica 89:325–337

    Google Scholar 

  • Shen Y, Meng D, McGrouther K, Zhang J, Cheng L (2017) Efficient isolation of Magnolia protoplasts and the application to subcellular localization of MdeHSF1. Plant Methods 13:44–54

    Article  PubMed  PubMed Central  Google Scholar 

  • Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured. Vitro Symp Soc Exp Biol 11:118–131

    PubMed  CAS  Google Scholar 

  • Skoog F, Tsui C (1948) Chemical control of growth and bud formation in tobacco stem segments and callus cultured in vitro. Forestry 45:197–210

    Google Scholar 

  • Sutter E, Langhans RW (1979) Epicuticular wax formation on carnation plantlets regenerated from shoot tip culture. J Am Soc Hort Sci 104:493–496

    Google Scholar 

  • Tahami SK, Chamani E, Zare N (2014) Plant regeneration from protoplasts of Lilium ledebourrii (Baker) Boiss. J Agric Sci Technol 16:1133–1144

    Google Scholar 

  • Tapingkae T, Zulkarnain Z, Kawaguchi M, Ikeda T, Taji A (2012) Somatic (asexual) procedures (haploids, protoplasts, cell selection) and their applications. In: Altman A, Hasegawa PM (eds) Plant biotechnology and agriculture: prospects for the 21st century. Academic Press, Oxford, pp 141–162

    Chapter  Google Scholar 

  • Tomiczak K, Mikula A, Rybczynski JJ (2015) Protoplast culture and somatic cell hybridization of gentians. In: Rybczynski JJ, Davey MR, Mikula A (eds) The Gentianaceae – volume 2: biotechnology and applications. Springer, Heidelberg/New York/Dordrecht/London, pp 163–186

    Chapter  Google Scholar 

  • Trigiano RN, Gray DJ (2005) Plant development and biotechnology. CRC Press LLC, Boca Raton, p 348

    Google Scholar 

  • Van Creij MGM, Kerckhoffs DMFJ, De Bruijn SM, Vreugdenhil D, Van Tuyl JM (2000) The effect of medium composition on ovary-slice culture and ovule culture in intraspecific Tulipa gesneriana crosses. Plant Cell Tissue Organ Cult 60:61–67

    Article  Google Scholar 

  • Van Tuyl J, De Jeu M (1997) Methods for overcoming interspecific crossing barriers. In: Shivanna K, Sawhney V (eds) Pollen biotechnology for crop production and improvement. University Press, Cambridge, UK, pp 273–292

    Chapter  Google Scholar 

  • Vazquez-Thello A, Yang L, Hidaka M, Uozumi T (1996) Inherited chilling tolerance in somatic hybrids of transgenic Hibiscus rosa-sinensis x transgenic Lavatera thuringiaca selected by double-antibiotic resistance. Plant Cell Rep 15:506–511

    Article  CAS  PubMed  Google Scholar 

  • Went FW (1929) On a substance, causing root formation. Proceedings Royal Academy, Amsterdam xxxii:35–39

    Google Scholar 

  • Zimmerman PW, Wilcoxon F (1935) Several chemical growth substances which cause initiation of root and other responses in plants. Contrib Boyce Thompson Inst 7:209–229

    CAS  Google Scholar 

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Correspondence to Mark P. Bridgen .

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Bridgen, M.P., Van Houtven, W., Eeckhaut, T. (2018). Plant Tissue Culture Techniques for Breeding. In: Van Huylenbroeck, J. (eds) Ornamental Crops. Handbook of Plant Breeding, vol 11. Springer, Cham. https://doi.org/10.1007/978-3-319-90698-0_6

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