Metallomics pp 139-181 | Cite as

Bioimaging Metallomics

  • Valderi Luiz Dressler
  • Edson Irineu Müller
  • Dirce Pozebon
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1055)

Abstract

This chapter focuses on bioimaging in metallomics, which involves metal and metalloids distribution in animal tissues. It starts with laser ablation-inductively coupled plasma-mass spectrometry followed by secondary ion mass spectrometry, synchrotron-based X-ray fluorescence, and electron microscopy, including transmission electron microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The basic principles of these techniques and their application for qualitative and quantitative imaging of elements are presented. Sample preparation for bioimaging is briefly discussed. The usefulness of element bioimaging is demonstrated for cells and several animal tissues, including the brain, liver, hair, eye, teeth, and bone. As such, this chapter addresses the state of the art in bioimaging metallomics.

Keywords

Metallomics Bioimaging LA-ICP-MS SIMS SRXRF SEM TEM  EDX 

Abbreviations

2D

Two dimensional

2D-PAGE

Two-dimensional polyacrylamide gel electrophoresis

3D

Three dimensional

AD

Alzheimer disease

AmD

Amyloid deposits

ATP

Adenosine triphosphate

BNCT

Boron neutron capture therapy

BSA

Bovine serum albumin

CCD

Charge-coupled device

CRM

Certified reference material

CT

Computed tomography

Da

Dalton

DNA

Deoxyribonucleic acid

DOTA

Tetraazacyclododecane-1,4,7,10-tetraacetic acid

DTPABMA

2-[bis[2-[carboxymethyl-[2-(methylamino)-2-oxoethyl]amino]ethyl]amino]acetic acid

EDX

Energy-dispersive X-ray spectroscopy

EEL

Energy loss

EELS

Electron energy loss spectroscopy

EF

Energy-filtered

EFTEM

Energy-filtered transmission electron microscopy

EM

Electron microscopy

ESI-MS

Electrospray-mass spectrometry

FEG

Field emission gun

FWHM

Full width at half maximum

GE

Gel electrophoresis

HPF

High-pressure freezing

ICP

Inductively coupled plasma

ICP-MS

Inductively coupled plasma mass spectrometry

ID

Isotope dilution

IEF-GE

Isoelectric focusing gel electrophoresis

IPG

Immobilized pH gradient strips

IR

Infrared

IS

Internal standard

LA

Laser ablation

LA-ICP-MS

Laser ablation-inductively coupled plasma mass spectrometry

LA-MS

Laser ablation mass spectrometry

LMIG

Liquid-metal ion gun

LOD

Limit of detection

MC-ICP-MS

Multicollector-inductively coupled plasma mass spectrometry

MeCAT

Metal-coded affinity tag

METTEM

Metal-tagging transmission electron microscopy

MLEM

Maximum likelihood expectation maximization

MRI

Magnetic resonance image

MSI

Mass spectrometry imaging

ND-IEF-GE

Non-denaturing isoelectric focusing gel electrophoresis

NINA

Nanoimaging and nanoanalysis

NPs

Nanoparticles

ODU

Optical density unit

PAGE

Polyacrylamide gel electrophoresis

QDs

Quantum dots

QMS

Quadrupole mass spectrometer

RAPTA

Ru-arene bound to 1,3,5-triaza-7-phosphotricyclo-[3.3.1.1] decane

REEs

Rare earth elements

ROI

Regions of interest

RSF

Relative sensitivity factor

SDS-PAGE

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis

SEC-ICP-MS

Size exclusion chromatography-inductively coupled plasma mass spectrometry

SEM

Scanning electron microscopy

SF-ICP-MS

Sector field-inductively coupled plasma mass spectrometry

SIMS

Secondary ionization mass spectrometry

SN-ICP-MS

Solution nebulization inductively coupled plasma mass spectrometry

SNR

Signal-to-noise ratio

SOD

Superoxide dismutase

SRXRF

Synchrotron X-ray fluorescence

STEM

Scanning transmission electron microscopy

STEM-EELS

Scanning transmission electron microscopy-electron energy loss spectroscopy

TEM

Transmission electron microscopy

ToF

Time of flight

ToF-ICP-MS

Time of flight-inductively coupled plasma mass spectrometry

ToF-SIMS

Time of flight-secondary ionization mass spectrometry

UV

Ultraviolet

UHV

Ultra high vacuum

VSOP

Very small iron oxide particles

WD

Wilson’s disease

WDS

Wavelength-dispersive spectrometry

XAFS

X-ray absorption fine structure

XANES

X-ray absorption near edge structure spectroscopy

XAS

X-ray absorption spectroscopy

XRF

X-ray fluorescence

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Valderi Luiz Dressler
    • 1
  • Edson Irineu Müller
    • 1
  • Dirce Pozebon
    • 2
  1. 1.Universidade Federal de Santa MariaSanta MariaBrazil
  2. 2.Universidade Federal do Rio Grande do SulPorto AlegreBrazil

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