Abstract
Alfalfa (Medicago sativa L.) is a major perennial forage legume crop with numerous nutritional and environmental benefits. This allogamous and autotetraploid species whose varieties are synthetic populations, has a highly polymorphic genome and a short linkage disequilibrium. Allele mining strategy on targeted candidate genes is an option to select valuable parents for breeding. In this paper, we assessed allelic diversity of CAD and WXP1 genes, involved in lignin biosynthesis and drought tolerance respectively, in a set of 384 individuals. For CAD which had two splice forms, 30 and 31 variants (out of 1077 and 906 base pairs of the coding sequences, respectively) have been observed including 37 and 52% of non-synonymous mutations. More variants were observed in WXP1 sequence, with 157 observed in WXP1 coding sequence (coding sequence of 1116 base pairs), including 60% non-synonymous mutations. Among the non-synonymous mutations, some probably affected protein function. Both genes were under purifying selection, especially CAD with dN/dS rate of 0.05 and 0.12 for both splice forms, against a rate of 0.26 for WXP1. Difference in variant proportion is probably explained by differential selective pressure that may be induced by contrasted expression levels. Indeed, CAD is highly and continually expressed whereas WXP1 is induced in some specific conditions. Further studies assessing the impact of variants on phenotype will help to conclude on the allele mining strategy in alfalfa breeding.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Baucher M, Monties B, Montagu MV, Boerjan W (1998) Biosynthesis and genetic engineering of lignin. Crit Rev Plant Sci 17:125–197
Baucher M, Bernard-Vailhé MA, Chabbert B, Besle J-M, Opsomer C, Montagu MV, Botterman J (1999) Down-regulation of cinnamyl alcohol dehydrogenase in transgenic alfalfa (Medicago sativa L.) and the effect on lignin composition and digestibility. Plant Mol Biol 39:437–447
Drummond DA, Bloom JD, Adami C, Wilke CO, Arnold FH (2005) Why highly expressed proteins evolve slowly. Proc Natl Acad Sci USA 102:14338–14343
Herrmann D, Barre P, Santoni S, Julier B (2010) Association of a CONSTANS-LIKE gene to flowering and height in autotetraploid alfalfa. Theor Appl Genet 121:865–876
Huyghe C, Vliegher AD, van Gils B, Peeters A (2014) Grasslands and herbivore production in Europe and effects of common policies. Editions Quae
Li X, Han Y, Wei Y, Acharya A, Farmer AD, Ho J, Monteros MJ, Brummer EC (2014) Development of an alfalfa SNP array and its use to evaluate patterns of population structure and linkage disequilibrium. PLoS ONE 9:e84329
Omer S, Harlow TJ, Gogarten JP (2017) Does sequence conservation provide evidence for biological function? Trends Microbiol 25:11–18
Pál C, Papp B, Hurst LD (2001) Highly expressed genes in yeast evolve slowly. Genetics 158:927–931
Poke FS, Vaillancourt RE, Elliott RC, Reid JB (2003) Sequence variation in two lignin biosynthesis genes, cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase 2 (CAD2). Mol Breed 12:107–118
Yang J-R, Liao B-Y, Zhuang S-M, Zhang J (2012) Protein misinteraction avoidance causes highly expressed proteins to evolve slowly. Proc Natl Acad Sci 109:5158–5159
Zhang J-Y, Broeckling CD, Blancaflor EB, Sledge MK, Sumner LW, Wang Z-Y (2005) Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). Plant J 42:689–707
Zhang J-Y, Broeckling CD, Sumner LW, Wang Z-Y (2007) Heterologous expression of two Medicago truncatula putative ERF transcription factor genes, WXP1 and WXP2, in arabidopsis led to increased leaf wax accumulation and improved drought tolerance, but differential response in freezing tolerance. Plant Mol Biol 64:265–278
Acknowledgments
PhD thesis of C. Gréard is supported by ANRT (French Ministry of Higher Education and Research), CIFRE Convention n°2015/1447.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer International Publishing AG, part of Springer Nature
About this paper
Cite this paper
Gréard, C., Barre, P., Flajoulot, S., Santoni, S., Julier, B. (2018). Genetic Diversity Assessment of Two Medicago sativa Genes: CAD and WXP1. In: Brazauskas, G., Statkevičiūtė, G., Jonavičienė, K. (eds) Breeding Grasses and Protein Crops in the Era of Genomics. Springer, Cham. https://doi.org/10.1007/978-3-319-89578-9_41
Download citation
DOI: https://doi.org/10.1007/978-3-319-89578-9_41
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-89577-2
Online ISBN: 978-3-319-89578-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)