Somatic Embryogenesis in Scots Pine (Pinus sylvestris L.)
Scots pine (Pinus sylvestris L.) can be propagated via somatic embryogenesis. However, the protocols available today are not sufficient for large-scale propagation. The method needs to be optimized in order to increase the initiation frequency of embryogenic cell lines giving rise to high quality cotyledonary embryos, and to improve somatic embryo germination. Protocols presented in this chapter have been used to carry out fundamental research, where the resulting knowledge will be valuable for improving culture conditions for large scale propagation of Pinus species via somatic embryogenesis.
We wish to thank Gunnar Flygh and Ravi Shah for their critical reading of the manuscript and comments.
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