Abstract
Scots pine (Pinus sylvestris L.) can be propagated via somatic embryogenesis. However, the protocols available today are not sufficient for large-scale propagation. The method needs to be optimized in order to increase the initiation frequency of embryogenic cell lines giving rise to high quality cotyledonary embryos, and to improve somatic embryo germination. Protocols presented in this chapter have been used to carry out fundamental research, where the resulting knowledge will be valuable for improving culture conditions for large scale propagation of Pinus species via somatic embryogenesis.
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References
Abrahamsson M, Valladares S, Larsson E, Clapham D, von Arnold S (2012) Patterning during somatic embryogenesis in Scots pine in relation to polar auxin transport and programmed cell death. Plant Cell Tiss Organ Cult 109:391–400
Abrahamsson M, Valladares S, Merino I, Larsson E, von Arnold S (2017) Degeneration pattern in somatic embryos of Pinus sylvestris L. In Vitro Cell Dev Biol Plant 53:86–96
Aronen T, Pehkonen T, Ryynänen L (2009) Enhancement of somatic embryogenesis from immature zygotic embryos of Pinus sylvestris. Scand J For Res 24:372–383
Bozhkov PV, von Arnold S (1998) Polyethylene glycol promotes maturation but inhibits further development of Picea abies somatic embryos. Physiol Plant 104:211–224
Burg K, Helmersson A, Bozhkov P, von Arnold S (2007) Developmental and genetic variation in nuclear microsatellite stability during somatic embryogenesis in pine. J Exp Bot 58:687–698
Gupta PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana). Plant Cell Rep 4:177–179
Häggman H, Jokela A, Krajnakova J, Kauppi A, Niemi K, Aronen T (1999) Somatic embryogenesis of Scots pine: cold treatment and characteristics of explant affecting induction. J Exp Bot 50:1769–1778
Högberg KA, Bozhkov PV, von Arnold S (2003) Early selection improves clonal performance and reduces intraclonal variation of Norway spruce plants propagated by somatic embryogenesis. Tree Physiol 23:211–216
Keinonen-Mettälä K, Jalonen P, Eurola P, von Arnold S, von Weissenberg K (1996) Somatic embryogenesis of Pinus sylvestris. Scand J For Res 11:242–250
Klimaszewska K, Trontin J-F, Becwar MR, Devillard C, Park Y-S, Lelu-Walter M-A (2007) Recent progress in somatic embryogenesis of four Pinus spp. Tree For Sci Biotechnol 1(1):11–25
Kvaalen H, Appelgren M (1999) Light quality influences germination, root growth and hypocotyl elongation in somatic embryos but not in seedlings of Norway spruce. In vitro Cell Dev Biol 35:437–441
Lelu-Walter M-A, Bastien C, Drugeault A, Gouez ML, Klimaszewska K (1999) Somatic embryogenesis and plantlet development in Pinus sylvestris and Pinus pinaster on medium with and without growth regulators. Physiol Plant 105:719–728
Lelu-Walter M-A, Bernier-Cardou M, Klimaszewska K (2008) Clonal plant production from self- and cross-pollinated seed families of Pinus sylvestris (L.) through somatic embryogenesis. Plant Cell Tiss Organ Cult 92:31–45
Lindgren D (2008) A way to utilize the advantages of clonal forestry for Norway spruce? Working papers of the Finnish Forest Research Institute 114: 08–15
Merino I, Abrahamsson M, Sterck L, Craven-Bartle Canovas F, von Arnold S (2016) Transcript profiling for early stages during embryo development in Scots pine. BMC Plant Biol 16:255
Nörgaard JV, Duran V, Johnsen Ö, Krogstrup P, Baldursson S, von Arnold S (1993) Variations in cryotolerance of embryogenic Picea abies cell lines and association to genetic, morphological and physiological factors. Can J For Res 23:2560–2567
Park YS, Lelu-Walter MA, Harvengt L, Trontin JF, MacEacheron I, Klimaszewska K, Bonga JM (2006) Initiation of somatic embryogenesis in Pinus banksiana, P. strobus, P. pinaster, and P. sylvestris at three laboratories in Canada and France. Plant Cell Tiss Organ Cult 86:87–101
Park Y-S, Beaulieu J, Bousquet J (2016) Multi-varietal forestry integrating genomic selection and somatic embryogenesis. In: Park Y-S, Bonga JM, Moon H-K (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NiFos), Seoul, Korea, pp 302–322
Pullman GS, Skryabina A (2007) Liquid medium and liquid overlays improve embryogenic tissue initiation in conifers. Plant Cell Rep 26:873–887
Pullman GS, Chase K-M, Skryabina A, Bucalo K (2008) Conifer embryonic tissue initiation: improvements by supplementation of medium with D-xylose and D-chiro-inositol. Tree Physiol 29:147–156
Raven PH, Evert RF, Eichhorn SE (1999) Biology of plants, 6th edn. © W.H. Freeman and Company/Worth Publishers. W.H. Freeman and Company, New York, USA
Swedish Forest Agency (2014) Swedish statistical yearbook of forestry. [online] (2014) Available from http://www.skogsstyrelsen.se/en/AUTHORITY/Statistics/Statistical-Yearbook-/Statistical-Yearbooks-of-Forestry/. 1 Jan 2016
von Arnold S, Clapham D (2008) Spruce embryogenesis. In: Suarez MF, Bozhkov PV (eds) Methods in molecular biology, plant embryogenesis. Humana Press, Totowa, NJ, vol. 427, pp 31–47
Walter C, Find JI, Grace LJ (2005) Somatic embryogenesis and genetic transformation in Pinus radiata. In: Jain SM, Gupta PK (eds) Protocols for somatic embryogenesis in woody plants. Springer, Netherlands, pp 11–24
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We wish to thank Gunnar Flygh and Ravi Shah for their critical reading of the manuscript and comments.
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Abrahamsson, M., Clapham, D., von Arnold, S. (2018). Somatic Embryogenesis in Scots Pine (Pinus sylvestris L.). In: Jain, S., Gupta, P. (eds) Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants. Forestry Sciences, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-89483-6_9
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