In this thesis I have presented the vast potential of iSCAT to study single-molecule dynamics at its three distinct domains: temporal resolution (Chap. 4), localisation precision (Chap. 5) and sensitivity (Chaps. 6 and 7). Here, the boundaries have been set by the available camera sensor technology and the design of the sample area, rather then by the technique itself as has been discussed in Chap. 2. As a result, future developments that push these boundaries will indubitably be intertwined with the arrival of new technology. Nevertheless, these advancements will only be incremental unless the underlying practical limitations of the technique are fully understood and adequately addressed.
- 1.Chen, B.C., et al.: Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution. Science 346, 1257998 (2014)Google Scholar