Single-Molecule Chemical Dynamics: Direct Observation of Physical Autocatalysis

  • Jaime Ortega Arroyo
Part of the Springer Theses book series (Springer Theses)


This chapter outlines the application of iSCAT as a label-free sensor to monitor and characterise the progress of an physical autocatalytic chemical reaction in situ.


  1. 1.
    Bissette, A.J., Fletcher, S.P.: Mechanisms of autocatalysis. Angew. Chem. Int. Ed. 52, 12800–12826 (2013)CrossRefGoogle Scholar
  2. 2.
    Ruiz-Mirazo, K., Briones, C., de la Escosura, A.e.s.: Prebiotic systems chemistry: new perspectives for the origins of life. Chem. Rev. 114, 285–366 (2013)Google Scholar
  3. 3.
    Stano, P., Luisi, P.L.: Achievements and open questions in the self-reproduction of vesicles and synthetic minimal cells. Chem. Commun. 46, 3639–3653 (2010)CrossRefGoogle Scholar
  4. 4.
    Szostak, J.W., Bartel, D.P., Luisi, P.L.: Synthesizing life. Nature 409, 387–390 (2001)CrossRefGoogle Scholar
  5. 5.
    Segre, D., Ben-Eli, D., Deamer, D.W., Lancet, D.: The lipid world. Orig. Life Evol. Biosph. 31, 119–145 (2001)Google Scholar
  6. 6.
    Walde, P.: Surfactant assemblies and their various possible roles for the origin(s) of life. Orig. Life Evol. B. 36, 109–150 (2006)CrossRefGoogle Scholar
  7. 7.
    Stano, P., Wehrli, E., Luisi, P.L.: Insights into the self-reproduction of oleate vesicles. J. Phys. Condens. Mat. 18, S2231 (2006)CrossRefGoogle Scholar
  8. 8.
    Berclaz, N., Müller, M., Walde, P., Luisi, P.L.: Growth and transformation of vesicles studied by ferritin labeling and cryotransmission electron microscopy. J. Phys. Chem. B 105, 1056–1064 (2001)CrossRefGoogle Scholar
  9. 9.
    Nguyen, R., Allouche, L., Buhler, E., Giuseppone, N.: Dynamic combinatorial evolution within self-replicating supramolecular assemblies. Angew. Chem. Int. Ed. 48, 1093–1096 (2009)CrossRefGoogle Scholar
  10. 10.
    Chungcharoenwattana, S., Ueno, M.: New vesicle formation upon oleate addition to preformed vesicles. Chem. Pharm. Bull. 53, 260–262 (2005)CrossRefGoogle Scholar
  11. 11.
    Chen, I.A., Szostak, J.W.: A kinetic study of the growth of fatty acid vesicles. Biophys. J. 87, 988–998 (2004)CrossRefGoogle Scholar
  12. 12.
    Bissette, A.J., Odell, B., Fletcher, S.P.: Physical autocatalysis driven by a bond-forming thiol-ene reaction. Nat. Commun. 5, 4607 (2014)CrossRefGoogle Scholar
  13. 13.
    Andrecka, J., Spillane, K.M., Ortega Arroyo, J., Kukura, P.: Direct observation and control of supported lipid bilayer formation with interferometric scattering microscopy. ACS Nano 7, 10662–10670 (2013)Google Scholar
  14. 14.
    Buhse, T., Nagarajan, R., Lavabre, D., Micheau, J.C.: Phase-transfer model for the dynamics of “micellar autocatalysis”. J. Phys. Chem. A 101, 3910–3917 (1997)CrossRefGoogle Scholar
  15. 15.
    Baumgart, T., Hess, S.T., Webb, W.W.: Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension. Nature 425, 821–824 (2003)CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.ICFO—The Institute of Photonic SciencesBarcelonaSpain

Personalised recommendations