Abstract
Noninvasive ocular imaging platforms are undeniably useful in identifying retinal abnormalities. The purpose of this study was to investigate a novel method for integrating information acquired from two independent imaging platforms, AF-SLO and SDOCT, in order to demonstrate retinal perturbations as a result of genetic or pharmacological manipulation. Two cohorts of mice were investigated, Nyx nob and C57BL/6 J. In Nyx nob mice, SLO revealed an atypical but variable amount of autofluorescent foci (AFF); SDOCT showed altered photoreceptor outer segment architecture. Naïve Nyx nob had significantly more AFF than C57BL/6 J, suggesting that Nyx nob have some predisposition for developing AFF. Interestingly, both findings were significantly ameliorated in diabetic Nyx nob mice as compared to the controls. These data were incorporated into a novel analysis plot comparing AF-SLO and SDOCT results. The integration of the qualitative changes and accompanying quantitative analysis approach described herein provide a sensitive means for detecting whether a mouse model is susceptible to degeneration before other hallmark indicators are observed.
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Acknowledgments
This work was supported by a CDA to ISS from the US Dept. of Veterans Affairs Biomedical Laboratory Research and Development Service, VA Merit Award I01BX002754 (ISS) and an unrestricted grant from the Research to Prevent Blindness to the Cleveland Clinic Lerner College of Medicine of Case Western Reserve University.
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The contents of this work do not represent the views of the United States Department of Veterans Affairs or the United States Government.
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1 Electronic Supplementary Material
Supplemental Fig. 21.1
Detailed description of SDOCT image processing used to obtain the photoreceptor outer segment signal slope (TIFF 1751 kb)
Supplemental Fig. 21.2
Bar graph comparisons for AF-SLO and SDOCT findings . Comparison of AFF in naïve 3-month-old C57BL/6 J and Nyx nob mice (a). Comparison of AFF in naïve 3-month-Nyx nob mice and CNTL- or STZ-treated Nyx nob mice (b). Comparison of signal slope in naïve C57BL/6 J and Nyx nob mice (c). Comparison of signal slope in naïve 3-month-old Nyx nob mice and CNTL- or STZ-treated Nyx nob mice (d). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 (TIFF 225 kb)
Supplemental Fig. 21.3
Representative SDOCT B-scans and longitudinal reflectance profiles (LRP) of the outer retina categorized by approximate AFF Number. Linear curve fits are overlaid on the LRP for each group, indicating the location utilized to determine the OS reflective signal slope (TIFF 8310 kb)
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Bell, B.A., Bonilha, V.L., Samuels, I.S. (2018). A Novel Approach for Integrating AF-SLO and SDOCT Imaging Data Demonstrates the Ability to Identify Early Retinal Abnormalities in Mutant Mice and Evaluate the Effects of Genetic and Pharmacological Manipulation. In: Ash, J., Anderson, R., LaVail, M., Bowes Rickman, C., Hollyfield, J., Grimm, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 1074. Springer, Cham. https://doi.org/10.1007/978-3-319-75402-4_21
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DOI: https://doi.org/10.1007/978-3-319-75402-4_21
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