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Cell Culture Technology pp 129–137Cite as

Mimicking Physiological Oxygen in Cell Cultures

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Abstract

This chapter will provide a concise overview of the history of mammalian in vitro cell culture from its first descriptions through to the modern era. A specific focus will be adopted to understand when the importance of oxygen control emerged within the field, and we will discuss some of the earliest examples. A description of the common and state-of-the-art systems designed to provide an approximation of physiological normoxia will follow before finally describing its application in the emerging field of stem cell biology, specifically pluripotent stem cells and bone marrow-derived mesenchymal stem cells.

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  • DOI: 10.1007/978-3-319-74854-2_8
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Fig. 8.1

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Author information

Authors and Affiliations

  1. Guy Hilton Research Centre, Institute of Science and Technology in Medicine, Keele University, Stoke on Trent, UK

    Nicholas R. Forsyth, Muhammad Ahmad, Mohammed Al Zubaidi, Rakad Al-Jumaily, Marwan Merkhan & Tina Dale

  2. Censo Biotechnologies, Roslin Biocentre, Roslin, UK

    Rachel Steeg

Authors
  1. Nicholas R. Forsyth
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  2. Rachel Steeg
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  3. Muhammad Ahmad
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  4. Mohammed Al Zubaidi
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  5. Rakad Al-Jumaily
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  6. Marwan Merkhan
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  7. Tina Dale
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Correspondence to Nicholas R. Forsyth .

Editor information

Editors and Affiliations

  1. Department of Biotechnology, University of Natural Resources & Life Sciences, Vienna, Austria

    Prof. Dr. Cornelia Kasper

  2. Department of Biotechnology, University of Natural Resources & Life Sciences, Vienna, Austria

    Dr. Verena Charwat

  3. Institute for Technical Chemistry, Gottfried Wilhelm Leibniz Universität, Hannover, Germany

    Dr. Antonina Lavrentieva

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Forsyth, N.R. et al. (2018). Mimicking Physiological Oxygen in Cell Cultures. In: Kasper, C., Charwat, V., Lavrentieva, A. (eds) Cell Culture Technology. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-319-74854-2_8

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