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Cell Culture Media

Part of the Learning Materials in Biosciences book series (LMB)

Abstract

The chapter provides an overwiev on the basic constituents of culture media for mammalian cells and their derivation. The relevance of these constituents for individual cell types from different tissues will be covered with a focus on stem and progenitor cells. Moreover, approaches toward the derivation of defined media and the supplementation of serum additives are described. The relevance of physical parameters such as osmolarity and buffer systems will be approached.

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  • DOI: 10.1007/978-3-319-74854-2_3
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Correspondence to Reinhard Henschler .

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This chapter is dedicated to my former Ph.D. student, Dietrich (Dieter) Möbest who brought with him much basic knowledge and a high interest in cell culture media. Due to a severe illness, Dieter could not further pursue his career after his postdoc time. His solid knowledge in biotechnology and specifically cell culture media and his constant optimism stimulated my own interest in the field and formed the basis of several joint studies investigating the role of culture medium in determining the fate of hematopoietic cells.

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Henschler, R. (2018). Cell Culture Media. In: Kasper, C., Charwat, V., Lavrentieva, A. (eds) Cell Culture Technology. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-319-74854-2_3

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