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Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins

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Book cover Membrane Proteins in Aqueous Solutions

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

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Summary

Because they stabilize most membrane proteins as compared to detergent solutions and they trap them under the form of small particles, amphipols (APols) appear as an attractive potential medium for solution-state NMR (sNMR) studies. sNMR studies of APol-trapped membrane proteins have yielded information on the conformation of the proteins, their areas of contact with the polymer, their dynamics, their accessibility to water, and the structure of protein-bound ligands. They have benefited from the diversification of APol chemical structures and the availability of deuterated APols. The advantages and constraints of working with APols are discussed and compared to those associated with other nonconventional media.

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    Jean-Luc Popot

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Popot, JL. (2018). Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins. In: Membrane Proteins in Aqueous Solutions. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-73148-3_10

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