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Polyketide Synthase–Nonribosomal Peptide Synthetase Hybrid Enzymes of Fungi

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Physiology and Genetics

Part of the book series: The Mycota ((MYCOTA,volume 15))

Abstract

Fungi are known to produce various nonribosomal peptide-derived compounds that exhibit useful bioactivities. However, understanding of how those fungal secondary metabolites are biosynthesized still remains limited. In this review, we focus on recent efforts in engineering select fungal species to make them amenable to efficient genetic modifications for identifying genes responsible for the biosynthesis of secondary metabolites. The fungi discussed in this review are Chaetomium globosum, Aspergillus fumigatus, A. niger, A. nidulans, and A. oryzae. This review also discusses how the engineered fungi are used in deciphering the mechanism of natural product biosynthesis, primarily through heterologous reconstitution of biosynthetic pathways of interest. In particular, potential involvement of enzymatic Diels–Alder reactions in the secondary metabolite biosynthesis is discussed in details. Compounds discussed here are cytochalasans, such as chaetoglobosins and cytochalasins, Sch 210972, equisetin, and pyrrolocins.

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Acknowledgments

We wish to thank the financial support from the Japan Society for the Promotion of Science (JSPS) Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers (No. G2604) (K.W.). This work was also supported in part by the Japan Society for the Promotion of Science (JSPS) (K.W., 15KT0068, 26560450), the Takeda Science Foundation (K.W.), the Institution of Fermentation at Osaka (K.W.), the Japan Antibiotics Research Association (K.W.), the Uehara Memorial Foundation (K.W.), and the Tokyo Biochemical Research Foundation (K.W.).

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  1. Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan

    Shinji Kishimoto, Yuichiro Hirayama & Kenji Watanabe

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  1. Shinji Kishimoto
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  2. Yuichiro Hirayama
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  3. Kenji Watanabe
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Correspondence to Kenji Watanabe .

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  1. Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Kaiserslautern, Germany

    Timm Anke

  2. Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Kaiserslautern, Germany

    Anja Schüffler

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Kishimoto, S., Hirayama, Y., Watanabe, K. (2018). Polyketide Synthase–Nonribosomal Peptide Synthetase Hybrid Enzymes of Fungi. In: Anke, T., Schüffler, A. (eds) Physiology and Genetics. The Mycota, vol 15. Springer, Cham. https://doi.org/10.1007/978-3-319-71740-1_12

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