Human Sperm Morphology Analysis using a Digital Holographic Microscope

  • Emil Fabian
  • Marzena Kamieniczna
  • Maciej Kurpisz
  • Ewa StachowskaEmail author
Conference paper
Part of the Advances in Intelligent Systems and Computing book series (AISC, volume 623)


We used a digital holographic microscope (DHM) to analyse human sperm morphology. Length, width and thickness of the sperm cell head were determined by an operator, using the images produced by the software provided with the DHM. We developed an algorithm to replace part of the activities of the operator. This algorithm was written in the programming language R and the package EBImage. The algorithm produced similar results, while reducing the time needed from six hours to two when using a standard procedure.


digital holography human sperm morphology image analysis 


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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Emil Fabian
    • 1
  • Marzena Kamieniczna
    • 2
  • Maciej Kurpisz
    • 2
  • Ewa Stachowska
    • 1
    Email author
  1. 1.Department of Metrology and Measurement Systems, Institute of Mechanical Technology, Faculty of Mechanical Engineering and ManagementPoznan University of TechnologyPoznańPoland
  2. 2.Institute of Human Genetics Polish Academy of SciencePoznańPoland

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