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Tomographic Reconstruction from Electron Micrographs

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Cellular Imaging

Part of the book series: Biological and Medical Physics, Biomedical Engineering ((BIOMEDICAL))

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Abstract

Reconstruction from a tilt series of electron micrographs is based on the assumption that each image represents a projection through the specimen, and that the 3D information can be recovered by “back-projecting” all the images in the correct geometry. We use algorithms that are integrative (back-projection or Fourier inversion) or iterative (algebraic or maximum entropy methods). In practical tomography, we can only record a finite set of images at a dose low enough to avoid radiation damage, yielding noisy tomograms with missing information. The quality of the tomograms depends on the algorithmic details, but also on the pre-processing of the tilt series images, and post-processing of the tomographic volume.

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  1. National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, 50 South Dr, Bethesda, MD, 20892, USA

    J. Bernard Heymann

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    Eric Hanssen

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Bernard Heymann, J. (2018). Tomographic Reconstruction from Electron Micrographs. In: Hanssen, E. (eds) Cellular Imaging. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-68997-5_8

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