Pseudomonas spp.

  • Douglas I. Johnson
Chapter

Abstract

  • Genomics:
    • Pseudomonas aeruginosa chromosome: 6,264,403 bp; 5570 predicted ORFs (Stover et al. 2000)

  • Cell morphology:
    • Rod-shaped cells (Fig. 25.1)

    • Flagellum: monotrichous (P. aeruginosa); other species can be lophotrichous:
      • Swimming motility

      • Important role in adherence; immunostimulatory

    • Type 4 pili:
      • Several pili at the same pole as the flagellum

      • Essential function in adherence; immunostimulatory

      • Responsible for twitching motility; due to attachment/retraction of pili

    • Capsule:
      • Can form an alginate-based pseudocapsule during chronic infections (see below)

    • Lipopolysaccharide (LPS):
      • Associated with inflammation and endotoxic shock

      • Also functions in adherence (see below)

  • Gram stain:
    • Gram negative

  • Growth:
    • Aerobes; catalase positive, oxidase positive; respiration (no fermentation)

    • Ubiquitous in environment; soil and water; transient microbiota in humans

    • Can infect animals, plants, and nematodes

    • Produces several pigments; can be useful for species identification
      • Pyocyanin: blue green
        • Redox-active metabolite; virulence factor

      • Pyoverdine: yellow green; fluorescent
        • Fe+3 siderophore (also pyochelin)

      • Pyorubin: red

      • Pyomelanin: brown black

    • Excellent biofilm formers: can tolerate poorly oxygenated atmospheres (see below)

    • >190 species; one major human pathogen – Pseudomonas aeruginosa

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • Douglas I. Johnson
    • 1
  1. 1.Department of Microbiology & Molecular GeneticsUniversity of VermontBurlingtonUSA

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