Development of Microbiological Analyzer Based on Coherent Fluctuation Nephelometry

  • A. S. Gur’ev
  • O. Yu. Kuznetsova
  • L. A. Kraeva
  • S. F. Rastopov
  • V. N. Verbov
  • I. A. Vasilenko
  • E. V. Rusanova
  • A. Yu. Volkov
Conference paper
Part of the Advances in Intelligent Systems and Computing book series (AISC, volume 658)

Abstract

Rapid antibiotic susceptibility testing of etiologically significant microflora is an actual problem in clinical microbiology. Microbiological analyzers based on traditional nephelometry have a number of limitations. Coherent fluctuation nephelometry (CFN) method is almost insensitive to cuvette quality and allows constructing microbiological analyzers of high usability providing detection of microbial growth starting from 103–104 CFU/ml. CFN based microbiological analyzers were used for rapid antibiotic susceptibility testing of bacterial isolates and urine microflora without isolation within 3-6 hours in two independents studies. In the first study, susceptibility of 18 clinical bacterial isolates to 5 broad-spectrum antibiotics was determined by the disk diffusing method (DDM), the serial dilutions method (SDM) and CFN-analyzer. The pairwise agreement between the results was from 74.6% to 84.6%, the agreement between all three methods was 70%. In the second study, susceptibility of bacteria cultures in 21 urine samples from patients with bacteriuria to 13 broad-spectrum antibiotics was determined by CFN-analyzer without isolating the bacteria, the corresponding isolates were tested by DDM. The agreement between the results was 87.8%. Thus, CFN-method provides the possibility to construct multichannel microbiological analyzers of high sensitivity and usability. Such analyzers allow rapid antibiotic susceptibility testing of both bacterial isolates and urine microflora without bacteria isolating. CFN-analyzer allows implementation of full cycle of analyses, from bacteriuria confirmation to antibiotic susceptibility testing on the day of urine samples collection.

Keywords

Coherent fluctuation nephelometry Microbiological analyzer Antibiotic susceptibility Bacteriuria Personalized medicine 

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References

  1. 1.
    Medina E., Pieper D.H.: Tackling Threats and Future Problems of Multidrug-Resistant Bacteria. Curr Top Microbiol Immunol 398, 3-33 (2016).Google Scholar
  2. 2.
    Davenport M., Mach K.E., Shortliffe L.M.D., Banaei N., Wang T.H., Liao J.C.: New and developing diagnostic technologies for urinary tract infections. Nat Rev Urol, 14(5), 296-310 (2017).Google Scholar
  3. 3.
    Fontana C., Favaro M., Bossa M.C., Minelli S., Altieri A., Pelliccioni M., Falcione F., Di.Traglia L., Cicchetti O., Favalli C.: Improved diagnosis of central venous catheter-related bloodstream infections using the HB&L UROQUATTRO™ system. Eur J ClinMicrobiol Infect Dis, 31(11), 3139-3144 (2012).Google Scholar
  4. 4.
    Bourbeau P.P., Ledeboer N.A.: Automation in clinical microbiology. J Clin Microbiol 51(6), 1658-65 (2013).Google Scholar
  5. 5.
    Chandrappa S, Dharmanna L, Shyama Srivatsa Bhatta U V, Sudeeksha Chiploonkar M, Suraksha M N, Thrupthi S,”Design and Development of IoT Device to Measure Quality of Water”, International Journal of Modern Education and Computer Science(IJMECS), Vol.9, No.4, pp.50-56, 2017.DOI:  10.5815/ijmecs.2017.04.06
  6. 6.
    Madan Lal, Lakhwinder Kaur, Savita Gupta,”Speckle Reduction with Edge Preservation in B-Scan Breast Ultrasound Images”, International Journal of Image, Graphics and Signal Processing(IJIGSP), Vol.8, No.9, pp.60-68, 2016.DOI:  10.5815/ijigsp.2016.09.08
  7. 7.
    Rastopov S.F.: Coherent fluctuation nephelometry: A high-sensitivity method for detecting particles in liquids. InstrumExp Tech, 54(6), 837–840 (2011).Google Scholar
  8. 8.
    U.S. EPA. Detection of Biological Suspensions Using Online Detectors in a Drinking Water Distribution System Simulator. U.S. Environmental Protection Agency, EPA/600/R-10/005 (2010).Google Scholar
  9. 9.
    Gur’ev A.S., Volkov A.Y., Dolgushin I.I., Pospelova A.V., Rastopov S.F., Savochkina A.Y., Sergienko V.I.: Coherent Fluctuation Nephelometry: A rapid method for urine screening for bacterial contamination. Bull Exp Biol Med, 159(1), 107-110 (2015).Google Scholar
  10. 10.
    Gur’ev A.S., Kuznetsova O.Y., Pyasetskaya M.F., Smirnova I.A., Belyaeva N.A., Verbov V.N., Volkov A.Y. Rapid urine screening for bacteriuria in children using microbiology analyzer, combining photometric and coherent fluctuation nephelometric methods. Journal of Infection and Immunity, 6(4), 395-398 (2016). (In Russian)Google Scholar
  11. 11.
    Guidelines for susceptibility testing of microorganisms to antibacterial agents (MУК 4.2.1890 04). Clinical Microbiology and Antimicrobial Chemotherapy, 6(4), 306-359 (2004). (In Russian)Google Scholar

Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • A. S. Gur’ev
    • 1
    • 2
    • 3
  • O. Yu. Kuznetsova
    • 4
  • L. A. Kraeva
    • 4
  • S. F. Rastopov
    • 2
    • 5
  • V. N. Verbov
    • 4
  • I. A. Vasilenko
    • 1
    • 6
  • E. V. Rusanova
    • 1
  • A. Yu. Volkov
    • 2
  1. 1.M.F.Vladimirsky Moscow Regional Clinical and Research Institute (MONIKI)MoscowRussian Federation
  2. 2.Medtechnopark LTDMoscowRussian Federation
  3. 3.Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency (FRCC PCM)MoscowRussian Federation
  4. 4.Pasteur Institute of Epidemiology and MicrobiologySaint PetersburgRussian Federation
  5. 5.A.M. Prokhorov General Physics Institute of the Russian Academy of SciencesMoscowRussian Federation
  6. 6.A.N.Kosygin Russian State University (Technologies. Design. Art)MoscowRussian Federation

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