A Continuous Flow Microelectrophoretic Module for Protein Separation

  • A. CapuanoEmail author
  • A. Adami
  • V. Mulloni
  • L. Lorenzelli
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 457)


In this paper, we are presenting the development of a microfluidic electrically driven SPLITT (Split flow thin fractionation) device, which can be used for online protein separation. The microfluidic network is realized by means of a three times laminated structure (thermo-compression lamination) of a thin dry film, Ordyl SY 355, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.


Sample preparation Protein separation Microfluidics Dry film SPLITT Electrophoresis 



This work has received funding from the European Union’s Seventh Framework Programme FP7-ICT-2013-10 under grant agreement No 610580, STREP Project “SYMPHONY”.


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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • A. Capuano
    • 1
    • 2
    Email author
  • A. Adami
    • 1
  • V. Mulloni
    • 1
  • L. Lorenzelli
    • 1
  1. 1.Fondazione Bruno KesslerTrentoItaly
  2. 2.University of TrentoTrentoItaly

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