A Continuous Flow Microelectrophoretic Module for Protein Separation
In this paper, we are presenting the development of a microfluidic electrically driven SPLITT (Split flow thin fractionation) device, which can be used for online protein separation. The microfluidic network is realized by means of a three times laminated structure (thermo-compression lamination) of a thin dry film, Ordyl SY 355, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.
KeywordsSample preparation Protein separation Microfluidics Dry film SPLITT Electrophoresis
This work has received funding from the European Union’s Seventh Framework Programme FP7-ICT-2013-10 under grant agreement No 610580, STREP Project “SYMPHONY”.
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