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A Continuous Flow Microelectrophoretic Module for Protein Separation

  • A. Capuano
  • A. Adami
  • V. Mulloni
  • L. Lorenzelli
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 457)

Abstract

In this paper, we are presenting the development of a microfluidic electrically driven SPLITT (Split flow thin fractionation) device, which can be used for online protein separation. The microfluidic network is realized by means of a three times laminated structure (thermo-compression lamination) of a thin dry film, Ordyl SY 355, which is patterned through a photolithographic technique. The device has been tested with a Bovine Serum Albumin (BSA) solution, through absorbance measurements with a spectrophotometer, with best achieved separation at the outlet of 40%, measured as relative concentration unbalance at output channels.

Keywords

Sample preparation Protein separation Microfluidics Dry film SPLITT Electrophoresis 

Notes

Acknowledgements

This work has received funding from the European Union’s Seventh Framework Programme FP7-ICT-2013-10 under grant agreement No 610580, STREP Project “SYMPHONY”.

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  • A. Capuano
    • 1
    • 2
  • A. Adami
    • 1
  • V. Mulloni
    • 1
  • L. Lorenzelli
    • 1
  1. 1.Fondazione Bruno KesslerTrentoItaly
  2. 2.University of TrentoTrentoItaly

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