High-Performance Paper Microfluidic Malaria Test for Low-Resource Settings
In this chapter, we describe the application of paper microfluidics to develop a high-performance malaria test appropriate for use in low-resource settings. Malaria ranks in the top three for infectious disease burden worldwide, resulting in about two million deaths per year. Gold standard diagnostic tests for malaria use multistep protocols that require laboratory facilities and are often not accessible to patients in the developing world. Lateral flow tests are simple bioassays that have been used in low-resource settings for decades but can lack the sensitivity needed for clinical utility. Using paper microfluidic tools to automate the multistep sample processing that is characteristic of laboratory tests, we have developed a signal-amplified immunoassay for malaria detection with a higher sensitivity and a lower limit of detection than conventional lateral flow tests. Alternative fluid control tools are described within the same malaria system, and their potential for use in other systems is discussed.
KeywordsPaper microfluidics Point-of-care diagnostic testing Immunoassays Malaria diagnosis Low-resource settings
- 1.The World Health Organization: World Health Statistics 2014. World Health Organization, Geneva (2014)Google Scholar
- 7.Kifude, C.M., Rajasekariah, H.G., Sullivan, D.J., Stewart, V.A., Angov, E., Martin, S.K., Diggs, C.L., Waitumbi, J.N.: Enzyme-linked immunosorbent assay for detection of Plasmodium falciparum histidine-rich protein 2 in blood, plasma, and serum. Clin. Vaccine Immunol. 15(6), 1012–1018 (2008)CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Wong, R., Tse, H. (eds.): Lateral Flow Immunoassay. Humana Press, New York (2010)Google Scholar
- 23.Fu, E., Liang, T., Spicar-Mihalic, P., Houghtaling, J., Ramachandran, S., Yager, P.: Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection. Anal. Chem. 84(10), 4574–4579 (2012)CrossRefPubMedPubMedCentralGoogle Scholar
- 24.Spicar-Mihalic, P., Toley, B., Houghtaling, J., Liang, T., Yager, P., Fu, E.: CO2 laser cutting and ablative etching for the fabrication of paper-based devices. J. Micromech. Microeng. 23, 067003 (2013) (6pp)Google Scholar