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Gas and Liquid Chromatography-Mass Spectrometry-Based Metabolic Profiling of Marine Angiosperm Zostera muelleri (Alismatales, Zosteraceae)

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Book cover Systems Biology of Marine Ecosystems

Abstract

Seagrasses are monocotyledonous marine flowering plants that are considered lungs of the sea and are the most intense carbon sinks on the planet, delivering a range of ecologically and economically valuable biological services. In this study, we report the chemical fingerprint of Zostera muelleri using an untargeted metabolomic approach. High-performance liquid chromatography-mass spectrometry (HPLC-MS) and gas chromatography-mass spectrometry (GC-MS) were performed to study the metabolic profile of Z. muelleri. A total of 98 metabolites belonging to various chemical classes including flavonoids, phenolics, lipids, fatty acids, sugar alcohols and amino acids were identified, including two characteristic marker compounds of the genus, zosteric acid and rosmarinic acid. Chromatographic profiling yield a comprehensive map for the chemical constituents of Z. muelleri, and this method can be used as an effective and convenient approach to gain insights into the chemical composition of other seagrasses.

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Acknowledgement

LC-MS analysis and tentative identification of molecules using MA database was carried out by UK and Dr. Adrian Lutz, Metabolomics Australia, School of BioSciences, University of Melbourne. Tentative annotations using Metlin database and interpretation of mass spectra was carried out by UK. MK collected the seagrass samples and processed them for GC and LC-MS analysis. GC-MS profiling was carried out by Dorothee R Hahne, UWA Centre for Metabolomics, The University of Western Australia. MK is grateful to the Australian Research Council for awarding him Discovery Early Career Research Award (DECRA Fellowship, DE150100461-2015).

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Author notes

  1. Unnikrishnan Kuzhiumparambil and Manoj Kumar contributed equally to this work.

Authors and Affiliations

  1. Climate Change Cluster, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia

    Unnikrishnan Kuzhiumparambil, Manoj Kumar & Peter Ralph

Authors
  1. Unnikrishnan Kuzhiumparambil
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  2. Manoj Kumar
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  3. Peter Ralph
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Correspondence to Unnikrishnan Kuzhiumparambil .

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Editors and Affiliations

  1. Climate Change Cluster, Faculty of Science, University of Technology Sydney (UTS), Sydney, New South Wales, Australia

    Manoj Kumar

  2. Climate Change Cluster, Faculty of Science, University of Technology Sydney (UTS), Sydney, New South Wales, Australia

    Peter Ralph

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Kuzhiumparambil, U., Kumar, M., Ralph, P. (2017). Gas and Liquid Chromatography-Mass Spectrometry-Based Metabolic Profiling of Marine Angiosperm Zostera muelleri (Alismatales, Zosteraceae). In: Kumar, M., Ralph, P. (eds) Systems Biology of Marine Ecosystems. Springer, Cham. https://doi.org/10.1007/978-3-319-62094-7_9

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