In Vitro Incubation of Ovarian Follicles of Cultured Siberian Sturgeon, Acipenser baerii Brandt: A Short Practical Implementation and Its Fundamentals

Chapter

Abstract

This chapter is a practical description of the implementation of the in vitro maturation competence (IVMC) bioassay which is the decision-maker tool to choose the appropriate time (and then the suitable physiological status of the female) to hormonally inject the female with the best probability to get good-quality ovulated eggs. Brief recalls of involved organs, tissues, endocrine pathways. A detailed description of the procedure with the support of illustrations of the main tools is provided. A specific section is devoted to the incubation medium. The chronology of the diverse steps of the procedure is provided as well as the needed tools. Some comments on applications are given.

Keywords

Siberian sturgeon Ovarian follicles Oocyte In vitro maturation competence (IVMC) Endocrine pathways Incubation medium 

References

  1. Goncharov B, Williot P, Le Menn F (1999) Morphological and physiological characteristics of the ovarian follicles of farmed Siberian sturgeon and their importance for predicting artificial reproduction success. Rus J Develop Biol 30(1):46–54Google Scholar
  2. Jalabert B (1976) In vitro oocyte maturation and ovulation in rainbow trout (Salmo gairdneri), northern pike (Esox lucius), and goldfish (Carassius auratus). J Fish Res Board Can 33(4):974–988CrossRefGoogle Scholar
  3. Lutes PB, Doroshov SI, Chapman F et al (1987) Morpho-physiological predictors of ovulatory success in white sturgeon, Acipenser transmontanus Richardson. Aquaculture 66:43–52CrossRefGoogle Scholar
  4. Nagahama Y (1987) Endocrine control of oocyte maturation. In: Norris DO, Jones RE (eds) Hormones and reproduction in fishes, amphibians and reptiles. Plenum Press, New York, pp 171–202CrossRefGoogle Scholar
  5. Nagahama Y, Yoshikuni M, Yamashita M et al (1995) Regulation of oocyte growth and maturation in fish. Curr Top Dev Biol 30:103–145CrossRefGoogle Scholar
  6. Williot P (1997a) Reproduction de l’esturgeon sibérien (Acipenser baerii Brandt) en élevage: gestion des génitrices, compétence à la maturation in vitro de follicules ovariens et caractéristiques plasmatiques durant l’induction de la ponte. Thèse no 1822, Université Bordeaux I, 227 pGoogle Scholar
  7. Williot P (1997b) Effects of incubation media on maturation of isolated ovarian follicles of Siberian sturgeon (Acipenser baerii Brandt) induced by sturgeon gonadotropic preparation or 17α, 20β, dihydroxy progesterone. Comp Biochem Physiol 118c:285–293Google Scholar
  8. Williot P (2002) Reproduction des esturgeons. In: Billard R (ed) Esturgeons et caviar. Lavoisier Tech et Doc, Paris, pp 63–90Google Scholar
  9. Williot P, Brun R, Rouault T et al (1991) Management of female spawners of the Siberian sturgeon, Acipenser baeri Brandt: first results. In: Williot P (ed) Acipenser. Cemagref Publ, Antony, France, pp 365–379Google Scholar

Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.AudengeFrance

Personalised recommendations