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Metagenome Analyses of Multispecies Microbial Biofilms: First Steps Toward Understanding Diverse Microbial Systems on Surfaces

  • Christel Schmeisser
  • Ines Krohn-Molt
  • Wolfgang R. StreitEmail author

Abstract

Microbial biofilms are the dominant form of life on earth. Most naturally occurring microbial biofilms are phylogenetically diverse containing bacteria, archaea, viruses, phages, and smaller eukaryotes such as fungi, which have learned to live together. There are many examples of beneficial biofilms, such as biofilms in the plant rhizosphere and the phyllosphere and as part of the human microbiome. In industries and hospitals, biofilms are often unwanted because they are associated with pathogenicity or they interfere with production processes. On the other hand, especially in industries, biofilms are also used as production systems. Despite their complexity in nature and makeup, there are some common traits of biofilms: they require a surface, either biotic or abiotic; they produce a polymeric matrix (EPS) consisting of different mixtures of polysaccharides, fatty acids, proteins, and DNA; and they are embedded into this structure. The EPS gives a structure and a house to the many microbes, and it allows the exchange of signaling molecules, nutrients, DNAs, RNAs, and other molecules between the cells. Biofilms are not static but rather dynamic systems that are perhaps early forms of multicellular systems. While previous research has mainly focused on research on mono-species biofilms, it was only in the last few years the first examples of polymicrobial and complex biofilms were characterized in detail, using metagenome- and NGS-based technologies. Thus, within this review, we will highlight what we have learned about life in multispecies and complex biofilms through metagenome technologies during the last decade.

Keywords

Metagenome analyses Bacterial biofilms 

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Christel Schmeisser
    • 1
  • Ines Krohn-Molt
    • 1
  • Wolfgang R. Streit
    • 1
    Email author
  1. 1.Division of Microbiology and Biotechnology, Biocenter Klein FlottbekUniversity of HamburgHamburgGermany

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