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Proceeding of the 1st International Conference on Tropical Agriculture pp 337–344Cite as

Characteristics of Alcaligenes sp. LS2T Heterotrophic and Aerobic Ammonium Removal for Potential Livestock’s Wastewater Treatment

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Abstract

Alcaligenes spp. was found to have the ability to grow and remove ammonium in the medium through the nitrification-denitrification process. This ability was also known as an ammonium treatment in organic wastewater including livestock’s wastewater. In order to further understand its potential to remove ammonium in livestock’s wastewater, microbial growth, ammonium, nitrite, and nitrate production of Alcaligenes sp. LS2T were investigated in phosphate medium at different carbon/nitrogen ratios (C/N ratios) along with constant airflow. Ammonium sulfate was used as sole nitrogen source with acetate and citrate as an organic carbon source in a separate medium. Results showed that Alcaligenes sp. LS2T could utilize ammonium as sole nitrogen source which associated with acetate and citrate as carbon source at different C/N ratios, resulting in ammonium removal and production of nitrite and nitrate at various concentrations in the medium. The highest ammonium removal was found in the acetate C/N 28 medium where 94.44% of initial ammonium was removed.

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References

  1. Joo, H.S., et al.: Characteristics of Ammonium Removal by Heterotrophic Nitrification-Aerobic Denitrification by Alcaligenes faecalis No. 4. J. Biosci. Bioeng. 100(2), 184–191 (2005)

    Article  CAS  PubMed  Google Scholar 

  2. Itokawa, H., et al.: Nitrous oxide production in high-loading biological nitrogen removal process under low COD/N ratio condition. Water Res. 35, 657–664 (2001)

    Article  CAS  PubMed  Google Scholar 

  3. Khin, T., Annachhatre, A.P.: Novel microbial nitrogen removal processes. Biotechnol. Adv. 22, 519–532 (2004)

    Article  CAS  PubMed  Google Scholar 

  4. Rostron, W.M., et al.: Nitrification of high strength ammonia wastewaters: comparative study of immobilization media. Water Res. 35, 1169–1178 (2001)

    Article  CAS  PubMed  Google Scholar 

  5. Jung, J.Y., et al.: Enhanced ammonia nitrogen removal using consistent biological regeneration and ammonium exchange of zeolite in modified SBR process. Water Res. 38, 347–354 (2004)

    Article  CAS  PubMed  Google Scholar 

  6. Neerackal, G.M., et al.: Potential application of Alcaligenes faecalis strain No.4 in mitigating ammonia emissions from dairy wastewater. Biores. Technol. 206, 36–42 (2016)

    Article  CAS  Google Scholar 

  7. Joo, H.S., et al.: Piggery wastewater treatment using Alcaligenes faecalis strain no.4 with heterotrophic nitrification and aerobic denitrification. Water Res. 40, 3029–3036 (2006)

    Article  CAS  PubMed  Google Scholar 

  8. Posmanik, R., et al.: Effect of high ammonia loads emitted from poultry-manure digestion on nitrification activity and nitrifier-community structure in a compost biofilter. Ecol. Eng. 62, 140–147 (2014)

    Article  Google Scholar 

  9. Pai, S., et al.: Potential application of aerobic denitrifying bacteria as bioagents in waste water treatment. Bioresour. Technol. 68, 179–185 (1999)

    Article  CAS  Google Scholar 

  10. Zhao, B., et al.: N2O and N2 production during heterotrophic nitrification by Alcaligenes faecalis strain NR. Bioresour. Technol. 116, 379–385 (2012)

    Article  CAS  PubMed  Google Scholar 

  11. Liu, Y., et al.: Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16. Chin. J. Chem. Eng. 23(5), 827–834 (2015)

    Article  CAS  Google Scholar 

  12. Gutama, R.: Karakteristik mikrobia Alcaligenes sp.LS2T dan kemampuannya dalam mereduksi senyawa amonia [Alcaligenes sp. LS2T microbial characteristics and ability to reduce ammonia]. Minithesis. Universitas Gadjah Mada. (2015.) [in Bahasa Indonesia]

    Google Scholar 

  13. APHA.: Standard methods for the examination of water and wastewater, 19th ed (1995). http://ajph.aphapublications.org/doi/pdf/10.2105/AJPH.85.8_Pt_2.P.164?cookieSet=1. Accessed 12 Jan 2016

  14. EPA.: Method 352.1: Nitrogen, Nitrate (Colorimetric, Brucine) by spectrophotometer (1971). https://www.epa.gov/sites/production/files/2015-08/.../method_352-1_1971.pdf. Accessed 22 Jan 2016

  15. Jetten, M.S.M.: Novel principles in the microbial conversion of nitrogen compounds. Antonie Van Leeuwenhoek. 71, 75–93 (1997)

    Article  CAS  PubMed  Google Scholar 

  16. Kuenen, J.G., Robertson, L.A.: Combined nitrification and dentrification processes. FEMS Microbiol. Rev. 15, 109–117 (1994)

    Article  CAS  Google Scholar 

  17. Gupta, A.B., Gupta, S.K.: Simultaneous carbon and nitrogen removal from high strength domestic wastewater in an aerobic RBC biofilm. Wat. Res. 35, 1714–1722 (2001)

    Article  CAS  Google Scholar 

  18. Joo, H.S., et al.: Improvement in ammonium removal efficiency in wastewater treatment by mixed culture of Alcaligenes faecalis No. 4 and L1. J. Biosci. Bioeng. 103, 166–173 (2007)

    Article  Google Scholar 

  19. Papen, H., von Berg, R.: Amost probable numbermethod (MPN) for the estimation of cell numbers of heterotrophic nitrifying bacteria in soil. Plant Soil. 199, 23–130 (1998)

    Article  Google Scholar 

  20. Yang, X.P., et al.: Isolation and nitrogen removal characteristics of an aerobic heterotrophic nitrifying–denitrifying bacterium, Bacillus subtilis A1. Bioresour. Technol. 102, 854–862 (2011)

    Article  CAS  PubMed  Google Scholar 

  21. Chen, Q., Ni, J.R.: Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria. J. Ind. Microbiol. Biotechnol. 38, 1305–1310 (2011)

    Article  CAS  PubMed  Google Scholar 

  22. Zhang, Q.L., et al.: The characteristics of a novel heterotrophic nitrification–aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour. Technol. 108, 35–44 (2012)

    Article  CAS  PubMed  Google Scholar 

  23. Witzel, K.P., Overbeck, H.J.: Heterotrophic nitrification by Arthrobacter sp. (strain 9006) as influenced by different cultural conditions, growth state and acetate metabolism. Arch. Microbiol. 122, 137–143 (1979)

    Article  CAS  Google Scholar 

  24. Weerayutsil, P., et al.: Optimum ratio of chicken manure and napier grass in single stage anaerobic co-digestion. Energy Procedia. 100, 22–25 (2016)

    Article  CAS  Google Scholar 

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Acknowledgments

This research was supported by Program Penelitian Unggulan (Prime Research Program) UGM 2016 DPKM Management contract number 690/UN1-P.III/LT/DIT-LIT/2016. The authors also thank Laboratory of Animal Skins, By-products, and Animal Wastes Technology, Faculty of Animal Science, Universitas Gadjah Mada, for providing research needs.

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Authors and Affiliations

  1. Faculty of Animal Science, Universitas Gadjah Mada, Jalan Fauna No. 3, Bulaksumur, Yogyakarta, 55281, Indonesia

    Aldyon Restu Azkarahman, Yuny Erwanto, Widodo Hadisaputro, Lies Mira Yusiati & Nanung Agus Fitriyanto

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  1. Aldyon Restu Azkarahman
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  2. Yuny Erwanto
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  3. Widodo Hadisaputro
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  4. Lies Mira Yusiati
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  5. Nanung Agus Fitriyanto
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Correspondence to Aldyon Restu Azkarahman .

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Editors and Affiliations

  1. Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, Indonesia

    Alim Isnansetyo

  2. Faculty of Biology, Universitas Gadjah Mada, Teknika Utara Sekip Selatan, Yogyakarta, Indonesia

    Tri Rini Nuringtyas

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Azkarahman, A.R., Erwanto, Y., Hadisaputro, W., Yusiati, L.M., Fitriyanto, N.A. (2017). Characteristics of Alcaligenes sp. LS2T Heterotrophic and Aerobic Ammonium Removal for Potential Livestock’s Wastewater Treatment. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_32

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