Characteristics of Alcaligenes sp. LS2T Heterotrophic and Aerobic Ammonium Removal for Potential Livestock’s Wastewater Treatment

  • Aldyon Restu AzkarahmanEmail author
  • Yuny Erwanto
  • Widodo Hadisaputro
  • Lies Mira Yusiati
  • Nanung Agus Fitriyanto
Conference paper


Alcaligenes spp. was found to have the ability to grow and remove ammonium in the medium through the nitrification-denitrification process. This ability was also known as an ammonium treatment in organic wastewater including livestock’s wastewater. In order to further understand its potential to remove ammonium in livestock’s wastewater, microbial growth, ammonium, nitrite, and nitrate production of Alcaligenes sp. LS2T were investigated in phosphate medium at different carbon/nitrogen ratios (C/N ratios) along with constant airflow. Ammonium sulfate was used as sole nitrogen source with acetate and citrate as an organic carbon source in a separate medium. Results showed that Alcaligenes sp. LS2T could utilize ammonium as sole nitrogen source which associated with acetate and citrate as carbon source at different C/N ratios, resulting in ammonium removal and production of nitrite and nitrate at various concentrations in the medium. The highest ammonium removal was found in the acetate C/N 28 medium where 94.44% of initial ammonium was removed.


Alcaligenes sp. LS2T Ammonium removal Heterotrophic nitrification 



This research was supported by Program Penelitian Unggulan (Prime Research Program) UGM 2016 DPKM Management contract number 690/UN1-P.III/LT/DIT-LIT/2016. The authors also thank Laboratory of Animal Skins, By-products, and Animal Wastes Technology, Faculty of Animal Science, Universitas Gadjah Mada, for providing research needs.


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Aldyon Restu Azkarahman
    • 1
    Email author
  • Yuny Erwanto
    • 1
  • Widodo Hadisaputro
    • 1
  • Lies Mira Yusiati
    • 1
  • Nanung Agus Fitriyanto
    • 1
  1. 1.Faculty of Animal ScienceUniversitas Gadjah MadaYogyakartaIndonesia

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