Morphology and Elemental Composition of Metal Based Granules in Wings of Bumblebees
The paper addresses detection and elemental analysis of solid metal-based particles/clusters present in wings of laboratory bread bumblebees. The goal of the article is detection and characterization of morphology and chemical composition of those particles. A combination of Gallium Focused Ion Beam (FIB)—Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) mapping were used for evaluation of particles/clusters localization in the samples. FIB allowed etching of subsurface structures and deeper understanding of particles/clusters structure. EDS mapping revealed distribution of single elements in detected particles/clusters. It was found that all evaluated particles/clusters were based on iron or iron oxides and were associated with calcium structures. Several other elements such as Al, Mg, Ti, Zn or Si were also detected. Several hypotheses for role of the detected particles were introduced, however the real purpose was not revealed.
KeywordsBiomineralisation Bumblebee Iron granules Magnetoreception
This paper was created on the Faculty of Metallurgy and Materials Engineering in the Project No. LO1203 “Regional Materials Science and Technology Centre—Feasibility Program” funded by Ministry of Education, Youth and Sports of the Czech Republic. The works were partially financed by project of students grant competition reg. no. SP2016/94 organized by Technical University of Ostrava, Faculty of Mining and Geology.
- 4.Cabalova L, Cabanova K, Bielnikova H, Kukutschova J, Dvorackova J, Dedkova K, Zelenik K, Kominek P (2015) Micro—and nanosized particles in Nasal Mucosa: a pilot study. Biomed Res IntGoogle Scholar
- 6.Edelman NB, Fritz T, Nimpf S, Pichler P, Lauwers M, Hickman RW, Papadaki-Anastasopoulou A, Ushakova L, Heuser T, Resch GP et al (2015) No evidence for intracellular magnetite in putative vertebrate magnetoreceptors identified by magnetic screening. Proc Natl Acad Sci 112:262–267CrossRefGoogle Scholar
- 11.Jandacka P, Alexa P, Pistora J, Trojkova J (2013). Hypothetical superparamagnetic magnetometer in a pigeon’s upper beak probably does not work. Eur Phys J E 36Google Scholar
- 12.Jandacka P, Burda H, Pistora J (2015). Magnetically induced behaviour of ferritin corpuscles in avian ears: can cuticulosomes function as magnetosomes? J R Soc Interface 12Google Scholar
- 20.Treiber CD, Salzer MC, Riegler J, Edelman N, Sugar C, Breuss M, Pichler P, Cadiou H, Sunders M, Lythgoe M et al (2012) Clusters of iron-rich cells in the upper beak of pigeons are macrophages not magnetosensitive neurons. Nature 484:367–370Google Scholar